Honeybee pupa polypeptide granules and preparation method thereof
A bee chrysalis and polypeptide technology, which is applied in food ingredients as antioxidants, functions of food ingredients, food ingredients containing natural extracts, etc., can solve the problems of lack of development, low extraction efficiency of active ingredients, waste of resources, etc., and achieve improvement Effects of improving immunity, memory enhancement, and extraction effect
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[0026] In the preparation method of described bee pupa polypeptide and bee pupa polypeptide granule, the preparation method of described bee pupa polypeptide comprises the following steps:
[0027] Step 1, degreasing, crushing and sieving the bee pupae to obtain powder, the powder is degreased with petroleum ether at 30-60°C, and air-dried to obtain defatted bee pupae powder;
[0028] Step 2, enzymatic hydrolysis, mixing each gram of the defatted bee pupae powder with 10-14 ml of protease at a temperature of 47-49°C and a pH value of 7.5-8.5, and then hydrolyzing for 3.5-4.5 hours to obtain a mixed solution ;
[0029] Step 3, inactivating the enzyme, placing the mixed solution at a temperature of 94-96° C., and standing it for 15-20 minutes to obtain an enzyme-inactivating solution;
[0030] Step 4, centrifuging, centrifuging the enzyme-inactivating solution at a speed of 7500-8500 r / min for 15-25 minutes, collecting the supernatant, and obtaining the bee pupa polypeptide;
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Embodiment 1
[0044] 1. Mix 13g bee pupa polypeptide, 10g soybean extract, 7g walnut extract, 5g sealwort extract, 5g golden cherry extract and 10g water to obtain bee pupa polypeptide solution;
[0045] 2. The bee pupa polypeptide solution was dried with hot air at a temperature of 40°C for 4 hours, and then pressed into granules to obtain the bee pupa polypeptide granule.
Embodiment 2
[0047] 1. Degreasing, crushing and sieving 13g of bee pupae to obtain powder, which was degreased and air-dried with petroleum ether at 45°C to obtain 10g of defatted bee pupae powder;
[0048] 2. Enzymolysis, mixing 10 g of the defatted bee pupae powder with 130 ml of protease at a temperature of 48°C and a pH value of 8, and then hydrolyzing for 4 hours to obtain a mixed solution;
[0049] 3. To inactivate the enzyme, place the mixed solution at a temperature of 95° C. and let it stand for 17 minutes to obtain an enzyme inactivation solution;
[0050] 4. Centrifuge, centrifuge the enzyme-inactivating solution at a speed of 8000r / min for 20 minutes, collect the supernatant, and obtain 7g of bee pupa polypeptide;
[0051] 5. Mix 7g bee pupa polypeptide with 5.4g soybean extract, 3.8g walnut extract, 2.7g sealwort extract, 2.7g golden cherry extract and 5.4g water to obtain bee pupa polypeptide solution;
[0052] 6. The bee pupa polypeptide solution was dried with hot air for ...
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