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Application of penem alcohol E1 derived from trichoderma citrinoviride in aspect of gastric cancer

A technology of penemalol and Trichoderma aurantii, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve problems such as drugs that have not yet been seen, and achieve significant anti-gastric cancer activity.

Inactive Publication Date: 2017-02-15
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity against gastric cancer, but there is no report on the chemical structure of the compound and its anti-proliferative activity against gastric cancer cells, so there is no drug related to it in the market

Method used

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  • Application of penem alcohol E1 derived from trichoderma citrinoviride in aspect of gastric cancer
  • Application of penem alcohol E1 derived from trichoderma citrinoviride in aspect of gastric cancer
  • Application of penem alcohol E1 derived from trichoderma citrinoviride in aspect of gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Fermentative production and separation and purification of the compound of embodiment 1

[0016] 1 Fermentation production

[0017] Fermentation culture of production bacteria: According to the conventional method of cultivating microorganisms, take Trichodermacitrinoviride. IBPT-4 (preserved in China Center for Type Culture Collection on January 25, 2013, address: Wuhan University, Wuhan, preserved The serial number is: CCTCC NO: M 2013055) Appropriate amount, inoculated on PDA solid slant medium and cultivated in 28°C incubator for 4 days.

[0018] Take an appropriate amount of Trichoderma citrinoviride. IBPT-4 cultured on a slant for 4 days, and inoculate it into 400 mL of culture solution [Culture solution composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0 , Glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCL 6.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermenta...

Embodiment 2

[0027] Example 2 Test of anti-tumor activity in vitro

[0028] 1 Experimental samples and experimental methods

[0029] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0030] Gastric cancer cell lines were used for subculture of cell lines and cells. Gastric cancer cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0031] Cell Proliferation Inhibitory Activity Test Method

[0032] Tetrazolium salt (MTT) method Take cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microlite...

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Abstract

The invention relates to application of penem alcohol E1 derived from trichoderma citrinoviride in the aspect of gastric cancer. The compound is structurally characterized in that a molecular skeleton of pentacycloamide is contained, a carbonyl is contained and connected with a decacarbon saturated fat long chain, a methyl is connected on N, and two hydroxyl groups exist in a molecule. Experiments verify that the alkaloid-type compound has high inhibition activity to gastric cancer cells. The penem alcohol E1 can be used for preparing gastric cancer cell proliferation inhibition drugs or anti-gastric-cancer drugs and can be used for antitumor study.

Description

technical field [0001] The invention relates to a kind of penemenol E1 derived from Trichoderma aurantii and its application in gastric cancer. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the process...

Claims

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Application Information

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IPC IPC(8): A61K31/4015A61P35/00C07D207/38C12P17/10
CPCA61K31/4015C12P17/10C07D207/38Y02A50/30
Inventor 陈立李欣欣刘沁颖李亚平毕延雪赵杨杨张其清
Owner FUZHOU UNIVERSITY
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