Traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and extraction method thereof

A technology for blue ear disease and swine flu, applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as the lack of research on compound prescriptions and the inability to comprehensively evaluate the effects of porcine reproductive and respiratory syndrome virus , to achieve the effects of not being resistant to drugs, regulating the immune function of the body, and improving clinical symptoms

Inactive Publication Date: 2017-02-22
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Looking at the current experimental research on the anti-virus of traditional Chinese medicine compounds, there are many studies on single prescriptions against swine influenza virus and porcine reproductive and respiratory syndrome virus respectively. At present, no compound prescription has been developed that can simultaneously resist swine influenza virus and porcine reproductive and respiratory syndrome virus. virus
Moreover, most of them are independently studied by in vivo test or in vitro test, which cannot comprehensively evaluate the effect of traditional Chinese medicine compound against swine influenza virus and porcine reproductive and respiratory syndrome virus.

Method used

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  • Traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and extraction method thereof
  • Traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and extraction method thereof
  • Traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The in vitro anti-porcine reproductive and respiratory syndrome virus (PRRSV) effect of embodiment 1 Chinese medicine compound preparation

[0048] 1. Determination of the median lethal dose (TCID50) of PRRSV on MARC‐145 cells

[0049] The virus to be tested was serially diluted 10 times in 10 concentration gradients, and the virus solution was added to a 96-well plate full of Marc-145 cells at a dose of 100 μl / well. Each gradient was repeated 8 times, and the last 2 columns were cells. For the control group, the virus liquid was discarded after adsorption at 37°C for 1.5 h, and 100 μl of maintenance liquid was added, and cultured in a CO2 cell incubator.

[0050] The cell lesion was observed every day, and the OD value of each well was measured by the MTT method after the lesion reached 75%. Growth inhibition rate of virus on cells=(OD value of cell control group-OD value of virus control group) / OD value of cell control group. The median toxic concentration (TCID50) ...

Embodiment 2

[0070] Example 2 Chinese medicine compound prevents and treats the mice infected with H1N1 and H3N2 subtype swine influenza virus

[0071] 1. H1N1 and H3N2 subtype swine influenza virus to chicken embryo half infectious dose (EID 50 ) determination

[0072] The virus liquid was sequentially diluted 10 times, and inoculated in the allantoic cavity of 10-day-old chicken embryos, each dilution was inoculated with 0.2ml / piece, and 4 replicates were made, and normal chicken embryos were set as a control at the same time. Place them in an incubator at 37°C for 72 hours, discard the dead embryos within 24 hours, harvest the allantoic fluid of the dead embryos after 24 hours and live embryos after 72 hours, and measure the titer of HA. Calculate EID according to the Reed-Muench method 50 .

[0073] 2. Effect of traditional Chinese medicine compound on pneumonia in mice infected with H1N1 and H3N2 subtype swine influenza virus

[0074] (1) Grouping of experimental animals

[0075]...

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Abstract

The invention relates to a traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and an extraction method thereof. The traditional Chinese medicine compound prescription comprises the following medicines in parts by weight: 15-25 parts of belamcanda rhizome, 15-25 parts of ephedra, 10-15 parts of lily root, 5-15 parts of asarum, 5-15 parts of Rhizoma Pinellinae Praeparata, 5-15 parts of radix bupleuri, 5-15 parts of radix scutellariae and 5-10 parts of licorice root. The traditional Chinese medicine compound prescription can simultaneously resist swine infuenza virus and porcine reproductive and respiratory syndrome virus, and especially H1N1 and H3N2 subtype swine infuenza viruses, has the advantages of lower or no toxic residues, small side effects, low tendency to generate drug resistance and the like, and is capable of regulating the immunologic functions of organisms, inhibiting virus duplication and improving clinical symptoms.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine for veterinary use, and relates to a Chinese medicine compound and an extraction method for simultaneously resisting swine flu and blue ear disease. Background technique [0002] Swine influenza (swine influenza, SI), also known as swine influenza, is an acute, febrile, highly contagious respiratory disease caused by swine influenza virus (swine influenza virus, SIV) of the Orthomyxoviridae family Influenzavirus A. Infectious diseases are clinically characterized by sudden onset, high fever, cough, dyspnea, recurrent attacks, high morbidity, and low mortality. The disease occurs all over the world, causing serious harm, huge economic losses, and a threat to human health. Its prominent epidemic feature is that it often exists in a subclinical form and often causes secondary or mixed infections of other respiratory bacteria and viruses, such as porcine reproductive and respiratory syndro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8967A61P31/16A61P31/14
CPCA61K36/88A61K36/17A61K36/233A61K36/268A61K36/484A61K36/539A61K36/8888A61K36/8967A61K2236/331A61K2236/39A61K2300/00
Inventor 王丙云刘樱何永明冼琼珍郑闳升计慧琴陈胜锋陈志胜张辉华张贝莹
Owner FOSHAN UNIVERSITY
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