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Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof

A nanofibrous membrane, periodontal tissue technology, applied in tissue regeneration, fiber treatment, non-woven fabrics, etc., can solve the threat of infectious diseases and various viral diseases, adverse cell adhesion growth, nutrient transport, difficult cytoplasmic Matrix structure and function and other issues, to achieve excellent cytocompatibility and blood compatibility, promote adhesion, proliferation and differentiation, and have a wide range of effects

Inactive Publication Date: 2017-02-22
DONGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of acellular matrix to prepare tissue-guided regeneration materials will have limited material sources, cumbersome materials and preparation processes, and is vulnerable to mammalian infectious diseases and various viral diseases.
Tissue-guided regenerative materials prepared with freeze-dried membranes of natural materials such as collagen are difficult to simulate the structure and function of the cytoplasmic matrix due to problems such as large pore size and poor connectivity of the pores, which is not conducive to cell adhesion growth and interstitial nutrition. transport of matter
In addition to natural materials, there are problems such as inflammatory reactions of degradation products when using synthetic degradable materials

Method used

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  • Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof
  • Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof
  • Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation method of blue shark skin collagen:

[0056] The first step: remove the muscle and fat tissue under the blue shark skin, wash and soak in distilled water for 0.5h. Cut the fish skin into pieces with a diameter of 3-5mm and filter for use;

[0057] Step 2: Put the fish skin in a 0.35mol / L NaOH solution for degreasing treatment, and stir for 6h with a magnetic stirrer. Store in a refrigerator at 4°C for 12h. After taking it out, clean and filter with distilled water;

[0058] The third step: Put the fish skin in an ethanol solution with a volume fraction of 85% for desugaring treatment, stir with a magnetic stirrer for 2 hours, take it out, and wash and filter with distilled water;

[0059] The fourth step: pulverize the fish skin with a grinder, place it in 0.35mol / L glacial acetic acid solution for hydrolysis, stir with a magnetic stirrer for 6 hours, and place it in a refrigerator at 4°C for 4 hours to obtain an extract;

[0060] Step 5: Using isoelectric point te...

Embodiment 2

[0063] A preparation method of blue shark skin collagen periodontal tissue guided regeneration nanofiber membrane, the specific steps are:

[0064] (1) Using the method in Example 1 to prepare blue shark skin collagen;

[0065] (2) Electrospinning using blue shark skin collagen, chitosan and PEO:

[0066] Add chitosan to 50% acetic acid aqueous solution, stir with a magnetic stirrer until it is completely dissolved. The concentration of chitosan in the solution is 2wt%, which is recorded as liquid A; in 90vol% hexafluoroisopropanol and 10vol The blue shark skin collagen prepared in step (1) was added to the mixed solution of% acetic acid, and stirred with a magnetic stirrer for 24 hours until it was completely dissolved. The concentration of blue shark skin collagen in the solution was 5.2 wt%, which was recorded as liquid B. Mix liquid A and liquid B in a volume ratio of 0.85:1, stir evenly with a magnetic stirrer, add PEO, stir again with a magnetic stirrer to make it completely d...

Embodiment 3

[0070] A preparation method of blue shark skin collagen periodontal tissue guided regeneration nanofiber membrane, the specific steps are:

[0071] (3) Using the method in Example 1 to prepare blue shark skin collagen;

[0072] (4) Electrospinning using blue shark skin collagen, chitosan and PEO:

[0073] The chitosan, the blue shark skin collagen prepared in step (1), and PEO are sequentially added to the 40% acetic acid aqueous solution, and stirred with a magnetic stirrer until completely dissolved to obtain a spinning solution. The concentration of chitosan in the solution was 1% by weight, the concentration of blue shark skin collagen was 3% by weight, and the concentration of PEO was 1% by weight. Add this spinning solution to the syringe and fix the syringe to the pusher. Set the spinning voltage to 16Kv, the spinning solution feed rate to be 0.6ml / h, and the receiving distance to be 20cm. Electrospinning under the conditions of an ambient temperature of 28°C and a relative...

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Abstract

The invention provides a blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and a preparing method thereof. The preparing method of the blue shark fishskin collagen periodontium guided regeneration nanofiber membrane includes the steps that blue shark fishskin collagen is prepared, and blue shark fishskin collagen or blue shark fishskin collagen and at least one of chitosan and PEO are used for carrying out electrostatic spinning to obtain the blue shark fishskin collagen periodontium guided regeneration nanofiber membrane. The blue shark fishskin collagen and chitosan in use are natural degradable materials and are excellent in biocompatibility and wide in source. The blue shark fishskin collagen periodontium guided regeneration nanofiber membrane prepared through the electrostatic spinning method can simulate the structure and functions of a natural cytoplasmic matrix well. The blue shark fishskin collagen periodontium guided regeneration nanofiber membrane is used for treating periodontium defects and can effectively promote cell adhesion, proliferation and differentiation and promote guided regeneration of periodontium.

Description

Technical field [0001] The invention belongs to the processing field of novel tissue repairing biological materials, and specifically relates to the preparation of blue shark skin collagen and the preparation of blue shark skin collagen periodontal tissue guided regeneration nanofiber membrane. Background technique [0002] Human health is easily threatened by various diseases, and oral diseases are one of the multiple diseases. Human oral cavity is very easy to cause oral trauma and tissue defects due to surgery and various common oral diseases. Periodontal disease is one of them. Periodontal disease refers to a disease that occurs in the tooth supporting tissue (periodontal tissue). As a common oral disease, it is one of the main causes of tooth loss in adults, and it is also a major oral disease that endangers human teeth and overall health. Therefore, the treatment of periodontal disease has always been a key clinical research area. At present, periodontal tissue guided reg...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/18A61L27/50A61L27/56D04H1/728D01D5/00
CPCA61L27/24A61L27/18A61L27/20A61L27/50A61L27/56A61L2430/34D01D5/0015D01D5/0069D01D5/0076D01D5/0092D04H1/728C08L89/06C08L71/02C08L5/08
Inventor 王璐蒋升赵新哲李选徐晓刚李铁军繆辉南王富军高晶
Owner DONGHUA UNIV
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