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Three-dimensional (3D) bio-printed brain tumor in vitro model and construction method thereof

A three-dimensional biological and construction method technology, applied in the fields of biochemical equipment and methods, microorganisms, tumors/cancer cells, etc., can solve the problems of insufficient cell nutrition supply, biological characteristics and function loss of brain tumor cells, etc., to solve the problem of cell nutrition The effect of insufficient supply

Active Publication Date: 2017-02-22
MEDPRIN REGENERATIVE MEDICAL TECH
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a three-dimensional bioprinted in vitro model of brain tumors. The in vitro model of brain tumors provided by the present invention can effectively solve the problem of cell nutrition inside the larger volume hydrogel in the prior art. Insufficient supply and loss of biological characteristics and functions of brain tumor cells in existing brain tumor models when cultured in vitro

Method used

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  • Three-dimensional (3D) bio-printed brain tumor in vitro model and construction method thereof
  • Three-dimensional (3D) bio-printed brain tumor in vitro model and construction method thereof
  • Three-dimensional (3D) bio-printed brain tumor in vitro model and construction method thereof

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Embodiment 1

[0062] Example 1 In vitro 3D bioprinting reconstruction of human glioma tissue

[0063] This example provides a method for constructing a three-dimensional bioprinted brain tumor in vitro model, figure 1 Schematic diagram of the process of reconstructing a glioma model for in vitro 3D bioprinting. The specific process steps are as follows:

[0064] (1) Hydrogel preparation

[0065] With hyaluronic acid / sodium alginate / gelatin as the reconstructed extracellular matrix and main support material, fibrinogen is added to promote cell viability and growth.

[0066] Firstly, the weighed hyaluronic acid, sodium alginate, gelatin and fibrinogen are packaged separately, and sterilized by γ-ray irradiation. Weigh in the ultra-clean bench, prepare 20ml each of 4% hyaluronic acid, 6% sodium alginate and 30% gelatin solutions, put them into 60ml centrifuge tubes, and seal them. At the same time, prepare a 3% fibrinogen solution for later use. Sodium alginate solution is difficult to dis...

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Abstract

The invention relates to a three-dimensional (3D) bio-printed brain tumor in vitro model and a construction method thereof. The brain tumor in vitro model is obtained from a brain tumor cell-containing hydrogel system by preparing hydrogel fibers and constructing with a 3D bio-printer, wherein the hydrogel fibers has a diameter of 0.16-0.75mm, and has a volume filling percentage in the brain tumor in vitro model of 40-90 percent; the brain tumor cell-containing hydrogel system is prepared from the following raw materials: hyaluronic acid, sodium alginate, gelatin, glutamine transaminase and brain tumor cells. According to the brain tumor in vitro model, the 3D structure can be well maintained in the later culturing process, the problems of insufficient nutrition supply for cells inside large-sized hydrogel and biological characteristic and function loss of brain tumor cells when an existing brain tumor model is subjected to in vitro two-dimensional (2d) culture can be effectively solved.

Description

technical field [0001] The invention relates to the technical field of medical basic research, in particular to a three-dimensional bioprinted brain tumor in vitro model and a construction method thereof. Background technique [0002] Human glioma is a highly malignant and highly recurrent brain tumor. Even the comprehensive treatment of surgery, radiotherapy and chemotherapy has not effectively improved the survival of patients. The report shows that the average survival time of patients with new-onset glioma is only 15 months, and generally relapses 5 to 7 months after surgery. Therefore, the treatment of glioma still faces great challenges. The new exploration of the mechanism and treatment of glioma will provide new hope for the treatment of glioma. [0003] For decades, research on tumors has remained limited to two-dimensional cell cultures and animal models. It is well known that tumor growth in vivo is a three-dimensional microenvironment structure, which not only...

Claims

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Application Information

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IPC IPC(8): C12N5/095
CPCC12N5/0695C12N2501/11C12N2501/115C12N2501/20C12N2501/72C12N2501/905C12N2513/00C12N2533/54C12N2533/74
Inventor 马骋袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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