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Method for preparing D-glucosylceramide by using soy sauce filter residue

A ceramide and glucosyl-based technology, which is applied in the field of preparing D-glucosyl ceramide, can solve the problems of low content and no economic value, and achieve the effects of improving economic benefits, improving resource utilization and developing high-value by-products

Active Publication Date: 2017-02-22
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Soy sauce filter residue is rich in soy sauce fermenting bacteria such as Aspergillus, and its bacterial wall contains high value-added components - glycosylceramide, but the content is very low, usually only accounting for 1-5 ppm of the dry weight of soy sauce filter residue, direct extraction is not economical value

Method used

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  • Method for preparing D-glucosylceramide by using soy sauce filter residue
  • Method for preparing D-glucosylceramide by using soy sauce filter residue

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A kind of method utilizing soy sauce filter residue to prepare D-glucosylceramide, adopts following steps:

[0025] A. Use an inoculation loop to measure 10 rings of Sphingomonas sp. strain, preservation number CICC10694, and 10 rings of Yarrowia lipolytica strain, preservation number CICC 1441; Inoculate into 100 mL activation medium respectively, and described activation medium is made up of the peptone of 0.50% mass ratio, 0.60% NaCl, 5% sucrose, the remainder distilled water; And culture 24 h in constant temperature incubator, culture temperature is At 28 ℃, the pH was controlled to 7.0, and the activated culture solution P of Sphingomonas sp. and the activated culture solution Q of Yarrowia lipolytica strain were obtained respectively, in which (Sphingomonas sp.) The concentration of the bacterial solution in the activated culture solution P of the strain is 1×105CFU / mL, and the concentration of the bacterial solution in the activated culture solution Q of the Yarr...

Embodiment 2

[0030] A kind of method utilizing soy sauce filter residue to prepare D-glucosylceramide, adopts following steps:

[0031] A. Use an inoculation loop to measure 15 strains of Sphingomonas sp., preservation number CICC10694, and 15 strains of Yarrowia lipolytica (Yarrowia lipolytica), preservation number CICC 1441; Inoculate into 100 mL activation medium respectively, the activation medium is composed of peptone of 0.50% by mass ratio, 0.60% of NaCl, 5% of sucrose, the rest of distilled water; At 28°C, the pH was controlled to 7.2, and the activated culture solution P of Sphingomonas sp. and the activated culture solution Q of Yarrowia lipolytica strain were respectively obtained, wherein (Sphingomonas sp.) The concentration of the bacterial solution in the activated culture medium P of the strain is 3×105CFU / mL, and the concentration of the bacterial solution in the activated culture medium Q of the Yarrowia lipolytica strain is 3×105CFU / mL;

[0032] B. Mix the activated cult...

Embodiment 3

[0036] A kind of method utilizing soy sauce filter residue to prepare D-glucosylceramide, adopts following steps:

[0037] A. Use an inoculation loop to measure 15 strains of Sphingomonas sp., preservation number CICC10694, and 15 strains of Yarrowia lipolytica (Yarrowia lipolytica), preservation number CICC 1441; Inoculate into 100 mL activation medium respectively, the activation medium is composed of 0.50% peptone by mass ratio, 0.60% NaCl, 5% sucrose, and the remaining distilled water; and cultivated in a constant temperature incubator for 72 h at a temperature of 26 ℃, control the pH to 7.2; respectively obtain Sphingomonas (Sphingomonas sp.) strain activated culture solution P and Yarrowia lipolytica (Yarrowia lipolytica) (Sphingomonas sp.) The concentration of the bacterial solution in the activated culture solution P of the strain is 7×105 CFU / mL, and the concentration of the bacterial solution in the activated culture solution Q of the Yarrowia lipolytica strain is 7×...

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Abstract

The invention relates to a method for preparing D-glucosylceramide by using a soy sauce filter residue, and is characterized in that the method comprises the following steps: activating strains: A, respectively inoculating 100 mL of an activation culture medium with an sphingomonas strain and a yarrowia lipolytica strain; B, carrying out aerobic fermentation culture of the sphingomonas strain activated culture liquid P and the yarrowia lipolytica strain activated culture liquid Q obtained in the step A for 36-72 h, to obtain a composite strain fermentation liquid; C, mixing the composite strain fermentation liquid with the soy sauce filter residue, adding the mixture into a compound fermentation tank, carrying out static culture, and culturing for 48-96 h at the temperature of 25-30 DEG C, to obtain a strain cell-rich filter residue; and carrying out high pressure homogenization for 2 times, to obtain a wall-broken cell homogenate; and D, mixing the wall-broken cell homogenate with an ethanol solution, filtering to obtain a precipitate, and thus obtaining a D-glucosylceramide extract. Through rational use of the soy sauce filter residue, the waste can be turned into treasure, soy sauce industries are facilitated to improve economic benefits, and the resource utilization of the soy sauce filter residue and the development of high-value by-products are greatly improved.

Description

[0001] field of invention [0002] The invention belongs to the technical field of biological fermentation, in particular to a method for preparing D-glucosylceramide by using soy sauce filter residue. Background technique [0003] my country is a big producer of soy sauce, and its current annual output has reached more than 5 million tons, accounting for more than 60% of the world's total annual output. With the rapid development of soy sauce industry, the treatment of soy sauce waste residue has become an urgent problem for soy sauce production enterprises. It is estimated that a soy sauce factory with an annual output of 500 tons can produce 150 tons of wet soy sauce residue. Traditionally, soy sauce filter residue has no economic value and is often discarded as waste. In fact, soy sauce filter residue is a "waste" worth developing. Soy sauce filter residue is rich in crude protein, crude fiber, crude fat and other active components, and is an ideal high-value conversion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/26C12R1/01C12R1/645
CPCC12P19/26C12P39/00
Inventor 周浩淼陈玉燕黄贤志陈斌王虹霞孙淑蓉蒋丽刚
Owner PROYA COSMETICS
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