Determination reagent for heart-type fatty acid binding protein and preparation method of determination reagent

A fatty acid combination and reagent technology, which is applied to the measurement of color/spectral characteristics, can solve the problems of poor quantitative accuracy, long operation time, and high price, and achieve the effects of high specificity, good stability, and easy operation

Inactive Publication Date: 2017-02-22
浙江达美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] (1) Mass spectrometry, the method is complex and expensive, and is not suitable for routine clinical practice
[0010] (2) Non-competitive sandwich

Method used

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  • Determination reagent for heart-type fatty acid binding protein and preparation method of determination reagent
  • Determination reagent for heart-type fatty acid binding protein and preparation method of determination reagent
  • Determination reagent for heart-type fatty acid binding protein and preparation method of determination reagent

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Embodiment 1

[0042] Main reagent used in this embodiment:

[0043] R1 reagent: phosphate buffer: pH7.2, 40mmol / L; bovine serum albumin: 50g / L.

[0044] R2 reagent: anti-human fatty acid binding protein monoclonal antibody (mouse) latex: 5ml / L.

[0045] 1) Preparation of R1 reagent

[0046] ①Pour 800ml of purified water into a suitable container.

[0047] ② Turn on the electric stirrer, and the stirring speed is 450 rpm.

[0048] ③Weigh 3.58g of dipotassium hydrogen phosphate and add it into the above purified water, stir until completely dissolved.

[0049] ④ Weigh 2.76g of potassium dihydrogen phosphate and add to the above solution, stir until completely dissolved.

[0050] ⑤Weigh 6.68g of bovine serum albumin and add to the above solution, stir until completely dissolved.

[0051] ⑦Stir for more than ten minutes

[0052] ⑧Set the volume to 1000ml.

[0053] 2) Preparation of R2 reagent

[0054] ①Pour 800ml of purified water into a suitable container.

[0055] ② Turn on the elect...

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Abstract

The invention relates to a determination reagent for heart-type fatty acid binding protein and a preparation method, and belongs to the technical field of methods for testing or analyzing materials by means of chemical or physical properties of determination materials. The determination reagent is prepared from a reagent R1 and a reagent R2, wherein the reagent R1 is a solution formed by dissolving bovine serum protein into purified water added with a buffer solution, and the reagent R2 is a solution formed by dissolving anti-human heart-type fatty acid binding protein monoclonal antibody (mouse) latex into purified water. When the determination reagent is applied to determination of the heart-type fatty acid binding protein, the determination reagent has the advantages of being rapid to determine, sensitive to determination, good in accuracy, high in specificity, good in stability and the like.

Description

technical field [0001] The invention relates to a reagent for measuring a heart-shaped fatty acid binding protein and a preparation method thereof, and belongs to the technical field of testing or analyzing materials by means of measuring their chemical or physical properties. Background technique [0002] Heart fatty acid binding protein (hFABP) is a novel small cytosolic protein abundant in the heart. It is highly cardiac specific (ie, predominantly expressed in cardiac tissue), but is also expressed at low concentrations in tissues other than the heart. After myocardial ischemic injury, hFABP can be found in the blood as early as 1-3 hours after the onset of chest pain, peak at 6-8 hours and the plasma level returns to normal within 24-30 hours. Cardiac fatty acid binding cytoplasmic protein consists of 132 amino acids with a molecular weight of 15 kDa. The heart fatty acid binding protein (hFABP) gene is located on chromosome I. It is one of the most abundant proteins...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 方朝君
Owner 浙江达美生物技术有限公司
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