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Spatial autofocusing laser confocal imaging Raman-spectrum detecting method and device

A Raman spectroscopy and detection device technology, which is applied in the fields of spatial optical imaging and spectral measurement, can solve the problems of single spectral detection mode and difficulty in improving the detection range of confocal Raman spectroscopy detection technology, and achieves the effect of improving spatial resolution.

Active Publication Date: 2017-02-22
BEIJING INFORMATION SCI & TECH UNIV
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Problems solved by technology

[0004] The present invention provides a space self-adjusting laser confocal imaging Raman spectrum detection method and device, aiming to solve the problems that the existing confocal Raman spectrum detection technology is difficult to improve the detection range and the spectrum detection mode is single

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  • Spatial autofocusing laser confocal imaging Raman-spectrum detecting method and device
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  • Spatial autofocusing laser confocal imaging Raman-spectrum detecting method and device

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Embodiment 1

[0040] In this embodiment, the dichroic light-splitting system 900 is an optical notch filter, the first light-splitting system 150 is a broadband light-splitting system with a certain light-splitting ratio, and the second light-splitting system 160 is a broadband light-splitting system with a certain light-splitting ratio.

[0041] Figure 4 It is a schematic diagram of Embodiment 1 of the spatial self-adjusting laser confocal imaging Raman spectroscopy detection method and device of the present invention.

[0042] Such as Figure 4 As shown, the spatial self-adjusting laser confocal imaging Raman spectroscopy detection method, the specific test method includes the following steps:

[0043] The laser 610 in the excitation beam system 600 generates excitation light, diverges and expands the beam through the negative lens 620 , and then collimates it into a parallel beam through the first condenser lens 630 .

[0044] The parallel light beam is reflected by the dichroic spect...

Embodiment 2

[0069] Figure 5 It is a schematic diagram of Embodiment 2 of the spatial self-adjusting laser confocal imaging Raman spectroscopy detection method and device of the present invention. Compared with Embodiment 1, this embodiment differs in that: Figure 5 As shown, the excitation beam system 600 is placed in the transmission direction of the dichroic spectroscopic system 900, the telephoto focusing system 100 is placed in the transmission direction of the dichroic spectroscopic system 900, and the first spectroscopic system 150 is placed in the dichroic spectroscopic system 900 for reflection direction.

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Abstract

The invention discloses a spatial autofocusing laser confocal imaging Raman-spectrum detecting method and a spatial autofocusing laser confocal imaging Raman-spectrum detecting device. According to the method and the device, through introducing a focusing telescopic technique and a confocal technique into spectral detection, separation is carried out on Rayleigh scattered light and Raman scattered light by utilizing a dichroic light splitting system; the automatic focus adjustment of a telescopic focusing system is precisely controlled by utilizing the characteristic that the maximum value of a confocal response curve of a detector precisely corresponds to a focal position and through searching and responding to the maximum value; exciting light beams are automatically focused on a to-be-detected object; meanwhile, spectral information of the focal position of a light spot of laser is obtained; meanwhile, through the light splitting system and through an imaging system and an image sensing system, image collection in the spatial area of the sample is obtained; the spatial autofocusing spectral detection and image obtaining are realized; a method and a device which are capable of realizing the spatial autofocusing imaging spectral detection of the sample are formed. The method and the device have the characteristics of automatic focusing and accurate positioning; the detection range is enlarged; the sensitivity of the spectral detection is improved.

Description

technical field [0001] The invention relates to the technical field of spatial optical imaging and spectral measurement, in particular to a method and device for detecting a Raman spectrum of a spatial self-adjusting laser confocal imaging. Background technique [0002] Laser confocal Raman spectroscopy testing technology is a new technology that combines spatial imaging technology and Raman spectroscopy analysis technology. It focuses the incident laser light on the sample through the self-adjusting telescopic focusing system, so that Without the interference of surrounding substances, the structure and composition of the material components of the photographed sample can be obtained, providing better molecular "fingerprint" characteristics. It can not only observe the Raman spectrum signals of different micro-regions in the same layer of the sample, but also observe the Raman signals of different layers of the sample space depth, and perform spatial scanning on the sample ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 祝连庆王帅姚齐峰张雯董明利娄小平骆飞
Owner BEIJING INFORMATION SCI & TECH UNIV
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