Method for fast measuring biosensor through glycerinum based on co-immobilizing technique

Abstract

The invention discloses a method for fast measuring a biosensor through glycerinum based on the co-immobilizing technique. The method comprises the following steps that S1, the co-immobilizing technique and process are developed, wherein screening of enzymes and carriers for immobilization and study of the co-immobilizing technique and method are included; S2, an optimum electrode system and a perfect matched reagent are developed; S3, the most effective biological reaction system is studied; S4, software development and hardware design are carried out, the enzyme electrode system and a flow injection analysis system are assembled together, matched software and hardware are combined, and the glycerinum fast measuring biosensor based on the co-immobilizing technique is obtained. The development process is detailed, the study methods are diversified, study data is true and reliable, the developed biosensor is high in detecting sensitivity, short in period, simple in operation process, low in use cost, high in detecting efficiency and wide in application range and consuming less manpower, and the influence of matter similar in structure on the detecting result is avoided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biosensor method for rapid determination of glycerol based on dual-enzyme immobilization technology. Background technique [0002] Glycerol is an important microbial carbon source. In fermentation production, glycerol as a carbon source is widely used in the cultivation of genetically engineered bacteria and the expression of biologically active macromolecules. If the concentration is too high, the expression of foreign proteins will be delayed, and if the concentration is too low, it will cause Poor or slow cell growth, prolong the high-density fermentation time. Therefore, the monitoring of the glycerol content in the fermentation broth is the key to ensure a high fermentation unit. Moreover, as an important biochemical product and platform compound, glycerol has a wide range of sources and applications. Whether it is the traditional saponification waste liquid distillation meth...

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Problems solved by technology

However, the glycerol kinase method is not suitable for color samples, and samples with complex components and many interferences; the spectrophotometric atomic absorption method is complicated to operate, has low experimental precision, takes a long time, and has large errors; gas chromatography, high performance liquid chromatography Although the method has high precision, the required instruments are expensive and professional operators are required to operate; the glycerin detection kit is accurate and fast, but it is expensive and costly, and is not suitable for the monitoring of glycerin content in domestic actual production

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