Method for detecting fatty acid in food

Abstract

The invention discloses a method for detecting fatty acid in food. The method includes grinding the food, sieving the ground food, filling a centrifuge tube with the sieved ground food, adding normal hexane, conducting heating extraction at 60 DEG C, adding a sodium methylate solution for methyl esterification, adding saturated sodium chloride, standing and taking liquid supernatant so as to obtain an analysis sample; detecting the analysis sample by gas chromatography and gas chromatography-mass spectrography to obtain retention time and a spectrum, and comparing the spectrum with a mass spectrum database to obtain a fatty acid components corresponding to different retention time; quantifying the peak area of corresponding fatty acid in the spectrum in terms of percentage, and computing and determining the percentage of each fatty acid component in the sample to be detected. The method for detecting the fatty acid in the food has the advantages of simplicity, convenience, less consumption of standard samples, simplified detection steps, shorter detection time, simple and feasible detection process, high repeatability and capability of being used for detecting relative content of the fatty acid in raw milk rapidly.

Description

technical field

[0001] The invention relates to the technical field of food detection, in particular to a method for detecting fatty acids in food. Background technique

[0002] Fatty acid (fatty acid) refers to a long aliphatic hydrocarbon chain containing a carboxyl group at one end. It is an organic substance. The general formula of a straight-chain saturated fatty acid is C (n) h (2n+ 1) COOH, the lower fatty acid is a colorless liquid with a pungent odor, and the higher fatty acid is a waxy solid with no obvious smell. Fatty acids are the simplest type of lipids and are the building blocks of many more complex lipids. Fatty acids can be oxidized and decomposed into CO in the presence of sufficient oxygen supply 2 and H 2 O, release a lot of energy, so fatty acid is one of the main energy sources of the body. Fatty acids have the advantage of being easy to digest and are a good source of energy needed by the human body. Among them, unsaturated fatty acids have cert...