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Process for immobilization of lipase

A kind of technology of lipase and triglyceride

Active Publication Date: 2017-02-22
BUNGE LODERS CROKLAAN BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these animal proteins are known to cause allergic reactions

Method used

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  • Process for immobilization of lipase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: with aqueous lipase D preparation of

[0027] Preparation of lipase solutions: Prepare seven lipase solutions according to Table 1. Sample N° 7 is a control sample. All reagents were mixed at room temperature at 150 rpm for 3-24 hours, and then the immobilized lipase pellet was collected by centrifugation.

[0028] Table 1

[0029]

Embodiment 2

[0030] Embodiment 2 (comparison)

[0031] All seven lipase preparations were subjected to acid hydrolysis at 60°C using the following acid hydrolysis reagents:

[0032] 1 g immobilized enzyme (use pellet after centrifugation)

[0033] 35g palm stearin fraction (raw)

[0034] 49g oleic acid

[0035] 0.126g H 2 o

[0036] Composition raw materials see Table 2

[0037] Table 2

[0038] raw material carbon number C48 62.1 C50 24.3 C52 8.6 C54 1.9 C56 0.0

[0039] Carbon numbers were determined by GC according to AOCS Ce 5.86.

[0040] Table 3 provides the results of the acid hydrolysis reaction of various starting materials after 24 hours.

[0041] table 3

[0042] HA OD BU HFA EA EP control carbon number C48 59.9 60.1 60.0 57.4 60.3 60.1 60.3 C50 25.4 25.3 25.3 26.8 25.1 25.3 25.2 C52 9.2 9.2 9.2 10.2 9.1 9.2 9.1 C54 2.1 2.1 2.2 2.5 2.1 2.1...

Embodiment 3

[0045] With the lipase preparation of 70ml embodiment 1 and 2.4g egg albumin, 0.65g Tween Mixed with 1.5 g of the respective supports. The acid hydrolysis reaction was carried out according to Example 2.

[0046] Table 4 shows the results of acid hydrolysis (24 hours) with various Saipa beads and aqueous lipase D solutions in the presence of egg albumin and Tween 20.

[0047] Table 4

[0048] HA OD BU HFA EA EP carbon number C48 7.2 9.5 61.2 6.9 7.2 61.4 C50 29.3 31.3 24.8 29.0 25.0 24.7 C52 41.5 41.5 8.8 42.8 42.3 8.8 C54 20.4 16.9 2.0 20.4 20.7 1.9 C56 0.5 0.4 0.0 0.5 0.4 0.0

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Abstract

A process is disclosed for immobilizing a lipase onto a support containing a functional amino group. The method comprises contacting the lipase with the support in the presence of a surface-active material.

Description

technical field [0001] The present invention relates to a method for immobilizing lipase on a support with amino functional groups, a method for producing a triglyceride composition using the immobilized lipase, and the application of lipase in transesterification. Background technique [0002] Lipases (E.C.3.1.1.3) belong to a family of enzymes that specifically catalyze the ester linkages of tri-, di- and monoacylglycerols to form glycerol and fatty acids. They also catalyze other reactions such as interesterification, esterification, acidolysis, alcoholysis, and aminolysis. The high cost of lipase makes the enzymatic reaction economically unattractive. Immobilization of lipase is a way to increase the industrial sensitivity of lipase and enable the recovery of lipase protein. Lipase can be immobilized on different supports by pre-treating the support or lipase in various ways. [0003] Nevena et al. (NEVENA, Z. Immobilization of lipase from Candida rugosa on Sepabeads:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12N11/08C12P7/64C12P7/6458
CPCC12N9/20C12N11/08C12Y301/01003C12P7/6458
Inventor K·巴格干J·马C·迈尔里尼V·塔兰
Owner BUNGE LODERS CROKLAAN BV