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Herbicide-tolerant plants and their applications

A herbicide and plant technology, applied in the fields of application, plant products, plant genetic improvement, etc., can solve problems such as long cycle time and long-term safety evaluation

Active Publication Date: 2019-06-21
深圳洁田模式生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, herbicide-resistant crop varieties bred by transgenic technology need to go through a long period of safety evaluation, and the cycle from successful variety selection to production and application is relatively long

Method used

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  • Herbicide-tolerant plants and their applications
  • Herbicide-tolerant plants and their applications
  • Herbicide-tolerant plants and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Construction of wheat mutant library and screening of herbicide-resistant mutants

[0032] Take commercially available wheat varieties—400kg of spring wheat Ningchun No. 4 seeds, 20kg of winter wheat Aikang 58 and Jimai 22 seeds, immerse the seeds in water for 4 hours, remove the seeds, and control the water. Then, the seeds were soaked with 0.5% (w / w) ethyl methanesulfonate (EMS) for 12 hours, ensuring that the liquid surface was submerged in the seeds. After 12 hours, discard the EMS solution, change the tap water to soak the seeds, discard the tap water, repeat the soaking 5 times, soak for 5 minutes each time, then rinse the seeds with tap water for 2 hours, turn the seeds during the washing process, so that the EMS is washed, and air-dried slightly After sowing, the plants were harvested after maturity. Among them, the spring wheat Ningchun No. 4 harvested 3500 kg of M2 generation (2nd generation of mutant library) seeds, and the two varieties of Aikang ...

Embodiment 2

[0034] Example 2. Molecular level identification of herbicide-resistant positive plants

[0035] In order to prove the relationship between the herbicide-resistant mutants obtained in the present invention and the acetyl hydroxy synthetase mutation in the plants, this example carried out molecular identification on the obtained herbicide-resistant positive plants.

[0036] The three acetyl hydroxyl synthase genes IMI1, IMI2 and IMI3 in the wheat genome are located in the D genome, B genome and A genome, respectively. Take the leaves of suspected herbicide-resistant positive plants obtained by screening, together with the leaves of wild-type plants, extract genomic DNA from individual plants, and use genomic DNA as a template to polymerize the three acetyl hydroxyl synthases IMI1, IMI2 and IMI3 encoded in the wheat genome. The full-length genomic DNA sequence was amplified by enzyme chain reaction, and the amplification primers were:

[0037] TaImi1-F:TAAAGAAGCTAGCCCAATCTGAAC ...

Embodiment 3

[0043] Example 3. Tolerance phenotype of imidazolinone herbicides in field offspring of herbicide-resistant positive plants

[0044] The progeny of the line identified as the homozygous mutant was collected as a single plant, and a plot of about one square meter was planted in the field, and the wild type was planted next to it as a control. When the wheat grows to the three-leaf stage, it is sprayed with imazethapyr at a concentration of 500 mg / L, which is twice the commercially recommended dose. After one month of spraying, we investigated the death of seedlings. We found that the wild-type control almost stopped growing after spraying imazethapyr, and the newly grown leaves were completely withered and yellow, and then gradually withered. Positive seedlings then all presented the phenotype of complete tolerance to imazethapyr, and their normal growth and development were hardly affected (see image 3 with Figure 4 ), finally jointing and earing normally. This indicates ...

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Abstract

The invention relates herbicide-tolerant plants and applications thereof, provides an ALS protein which enables the plants to have herbicide tolerance, and related nucleic acids, expression cassettes, carriers, cells, plants, and applications and methods for obtaining the herbicide-tolerant plants, and also provides a method of identifying the plants in the invention.

Description

technical field [0001] The invention belongs to the field of plant herbicide resistance, in particular, the invention relates to a protein capable of imparting herbicide resistance (tolerance) to plants (especially wheat) and its application in plant breeding and weed management. Background technique [0002] In agricultural production, weeds in farmland are one of the most important factors affecting crop yield. Weeds compete with crops for sunlight, fertilizer, water and space, reduce crop yield and quality, breed diseases and insect pests, and hinder agricultural operations. The application of herbicides is a modern advanced technology in agriculture, which has greatly promoted the control of weeds in farmland. However, due to the close relationship between crops and some farmland weeds, how to selectively kill weeds without harming crops has become an important problem in the application of herbicides. In response to this situation, developing new varieties of herbicide...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12N15/82A01H5/00A01H6/46C12Q1/6895G01N33/68
CPCC12N9/1022C12N15/8205C12N15/8274C12N15/8278C12Y202/01006
Inventor 王峥周宽基马力耕邓兴旺
Owner 深圳洁田模式生物科技有限公司
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