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Preparation method of foot-and-mouth disease vaccine

一种口蹄疫疫苗、口蹄疫病毒的技术，应用在兽用生物制品领域，能够解决产品品质、生产效率和生产成本不平衡等问题

Active Publication Date: 2017-03-08
SHANGHAI SHEN LIAN BIOMEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the simple combination of such separation and purification processing units is difficult to exert the best separation and purification efficiency of each processing unit, resulting in an extremely unbalanced situation in product quality, production efficiency and production cost, which is undesirable

Method used

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  • Preparation method of foot-and-mouth disease vaccine
  • Preparation method of foot-and-mouth disease vaccine
  • Preparation method of foot-and-mouth disease vaccine

Examples

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preparation example Construction

[0030] On the one hand, the application provides a method for preparing a foot-and-mouth disease vaccine, comprising the steps of: (i) obtaining the foot-and-mouth disease virus cell culture fluid; (ii) separating the foot-and-mouth disease virus cell culture fluid through a double-membrane combined filter system Purification, the dual-membrane integrated filtration system includes a microfiltration device and an ultrafiltration device arranged in parallel, the microfiltration device includes a microfiltration feed liquid tank, a first main pump and a microfiltration membrane, and the ultrafiltration device includes Concentrating tank, the second main pump and ultrafiltration membrane; Wherein said microfiltration device is used for removing the large particle impurity in said foot-and-mouth disease virus cell culture fluid, and said ultrafiltration device is used for removing described foot-and-mouth disease virus cell culture fluid The micromolecule impurity; described microf...

Embodiment 1

[0088] This embodiment provides a kind of preparation method of foot-and-mouth disease vaccine, adopts double-membrane combined with integrated filtration system to purify foot-and-mouth disease virus cell culture fluid, concrete steps are as follows:

[0089] (1) press figure 1 To build a dual-membrane integrated filtration system, use pH 7.6 PBS buffer solution to balance the microfiltration membrane (pore size: 0.2μm, inner diameter: 1mm, membrane area: 110cm 2 ) and ultrafiltration membrane (pore size: 500kD, inner diameter: 1mm, membrane area: 110cm 2 ), and the shear rate of the microfiltration membrane and the ultrafiltration membrane are all controlled at 4000s -1 , control the temperature at 2-10°C, and at the same time control the flux of the microfiltration membrane and ultrafiltration membrane at 63LMH, and maintain 58mL of PBS buffer solution with pH7.6 in the concentration tank;

[0090] (2) According to 80L / m 2 Material / membrane area ratio, the foot-and-mouth...

Embodiment 2

[0096] This embodiment provides a kind of preparation method of foot-and-mouth disease vaccine, adopts double-membrane combined with integrated filtration system to purify foot-and-mouth disease virus cell culture fluid, and utilizes chromatographic method to carry out further purification (i.e., refining), concrete steps are as follows:

[0097] (1) press figure 2 To build a dual-membrane integrated filtration system, use pH 7.6 PBS buffer solution to balance the microfiltration membrane (pore size: 0.45μm, inner diameter: 1mm, membrane area: 110cm 2 ) and ultrafiltration membrane (pore size: 300kD, inner diameter: 1mm, membrane area: 110cm 2 ), and the shear rate of the microfiltration membrane and the ultrafiltration membrane are all controlled at 1500s -1 , control the temperature at 2-10°C, and at the same time control the flux of the microfiltration membrane and ultrafiltration membrane at 36LMH, add 58mL of PBS buffer solution with pH7.6 in advance in the concentratio...

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Abstract

The invention relates to a preparation method of a foot-and-mouth disease vaccine. The method comprises the following steps: (i) obtaining a foot-and-mouth disease virus cell culture solution; (ii) performing separation and purification on the foot-and-mouth disease virus cell culture solution by a double-membrane combined-use integral filtering system; (iii) collecting concentrated liquid containing the foot-and-mouth disease virus, obtained in the step (ii). The invention also relates to the foot-and-mouth disease vaccine prepared by the method, and a purpose of using the foot-and-mouth disease vaccine to prepare medicines for preventing animal foot-and-mouth diseases. The invention also relates to a device used for preparing the foot-and-mouth disease vaccine. The device comprises the double-membrane combined-use integral filtering system.

Description

technical field [0001] The application relates to the field of veterinary biological products, in particular, the application relates to a method for preparing a foot-and-mouth disease vaccine, a foot-and-mouth disease vaccine prepared by the method, and the use of the foot-and-mouth disease vaccine in preparing medicines for preventing animal foot-and-mouth disease , and a device for preparing a foot-and-mouth disease vaccine. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious zoonotic disease caused by foot-and-mouth disease virus. Foot-and-mouth disease mainly affects cloven-hoofed animals, and its clinical diagnosis is characterized by blisters on the oral mucosa, hooves and breast skin. A variety of foot-and-mouth disease vaccines have been developed for the control and prevention of foot-and-mouth disease. For the preparation method of foot-and-mouth disease vaccine, the current field usually adopts a simple combination...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61P31/14B01D36/00
CPCA61K39/12B01D36/00A61K2039/5252A61K2039/552C12N2770/32134A61P31/14C12N2770/32151C12N2770/32163C12N2770/32171A61K2039/55566A61K39/135
Inventor 马贵军张震聂东升罗盘棋
Owner SHANGHAI SHEN LIAN BIOMEDICAL CORP
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