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A method for extracting polypeptide from distiller's grains

A technology for distiller's grains and water-soluble protein, which is applied in the field of extracting polypeptides from distiller's grains, can solve problems such as low yield of polypeptides, and achieve the effects of improving enzymatic hydrolysis effect, ensuring yield and avoiding restraint

Active Publication Date: 2019-10-08
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Even so, the yields of polypeptides prepared from oil meal in the above prior art are all low

Method used

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  • A method for extracting polypeptide from distiller's grains

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Step 1. Vacuum freeze-drying and pulverizing the wet distiller's grains.

[0060] Step two, degreasing. Wash the crushed slag with an organic solvent (such as acetone) for 2-3 times, and air-dry.

[0061] Step 3, water-soluble protein extraction. The distiller's grains are dissolved in an ethanol alcohol aqueous solution with a mass fraction of 80wt% by using lye, the pH is kept at 13 by adding the lye, and the solid is settled by centrifugation to obtain a filtrate. Then the filtrate is concentrated to 10% of the original volume with a 600-mesh filter membrane, then an acid solution is added to adjust its pH to 6, and a 600-mesh filter membrane is used to filter to obtain a solid component; the solid component is dissolved in water, and then Centrifuge to dry the solid phase.

[0062] Step 4, the above-mentioned solid is added in the fermenter, and the Trichoderma reesei liquid (the number of viable bacteria is 3 × 10 10 fu / ml), Bacillus amyloliquefaciens liquid (t...

Embodiment 2

[0067] Step 1. Vacuum freeze-drying and pulverizing the wet distiller's grains.

[0068] Step two, degreasing. Wash the crushed slag with an organic solvent (such as acetone) for 2-3 times, and air-dry.

[0069] Step 3, water-soluble protein extraction. The distiller's grains are dissolved in ethanol alcohol aqueous solution with a mass fraction of 50wt% by using lye, the pH is maintained at 8 by the addition of lye, and the solid is settled by centrifugation to obtain a filtrate. Then the filtrate is concentrated to 5% of the original volume with a 400 mesh filter membrane, then acid solution is added to adjust its pH to 2, and a 40000 mesh filter membrane is used to filter to obtain a solid component; the solid component is dissolved in water, and then Centrifuge to dry the solid phase.

[0070] Step 4, the above-mentioned solid is added in the fermenter, and the Trichoderma reesei liquid (the number of viable bacteria is 5 × 10 10 fu / ml), Bacillus amyloliquefaciens liqu...

Embodiment 3

[0075] Step 1. Vacuum freeze-drying and pulverizing the wet distiller's grains.

[0076] Step two, degreasing. Wash the crushed slag with an organic solvent (such as acetone) for 2-3 times, and air-dry.

[0077] Step 3, water-soluble protein extraction. The distiller's grains are dissolved in 60 wt% ethanol alcohol aqueous solution with lye, the lye is added to keep the pH at 10, and the solid is settled by centrifugation to obtain a filtrate. Then the filtrate is concentrated to 10% of the original volume with a 400-mesh filter membrane, then an acid solution is added to adjust its pH to 2, and a 400-mesh filter membrane is used to filter to obtain a solid component; the solid component is dissolved in water, and then Centrifuge to dry the solid phase.

[0078] Step 4, the above-mentioned solid is added in the fermenter, and the Trichoderma reesei liquid (the number of viable bacteria is 5 × 10 10 fu / ml), Bacillus amyloliquefaciens liquid (the number of viable bacteria is...

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Abstract

The invention discloses a method for extracting polypeptide from vinasse. The method includes: (1) degreasing the vinasse; (2) extracting water-soluble protein; (3) subjecting the degreased vinasse to fermentation by the aid of mixed bacterium of Trichoderma reesei, bacillus amyloliquefaciens, bacillus subtilis and Pasteur bacillus licheniformis; (4) subjecting the extracted water-soluble protein and the processed vinasse obtained from the step (3) to enzymolysis by the aid of protease. According to the method, the vinasse is fermented by the mixed bacterium of the Trichoderma reesei, the bacillus amyloliquefaciens, the bacillus subtilis and the Pasteur bacillus licheniformis before protease enzymolysis, wood fibers, semi-fibers and the like in the vinasse can be degraded into micro molecules like polysaccharides, celluloses and the semi-fibers are prevented from constraining the protein, full contact of the protein and the protease is enlarged, an enzymolysis effect of the protein is improved, and a yield rate is guaranteed.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a method for extracting polypeptide from distiller's grains. Background technique [0002] Distiller's grains, also known as red grains, distiller's grains, dregs, etc., are the residues left after brewing rice, wheat, sorghum, etc. Distiller's grains are rich in protein and are a very high source of protein. For a long time, most manufacturers mainly sell the wet grains directly as roughage at a low price, with little profit; a few manufacturers directly discharge the brewer's grains, which not only causes serious environmental pollution, but also leads to a waste of resources. Therefore, the development and comprehensive utilization of brewer's grains has become an important task for researchers. [0003] Polypeptide is a compound formed by connecting amino acids with peptide bonds, and it is also an intermediate product of protein hydrolysis. Many active subs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12R1/07C12R1/125C12R1/10C12R1/885
Inventor 郭鹏王轶
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI