The invention relates to an electromyography acquisition and amplification minisize sensor, belonging to an electromyographic signal acquisition device. The electromyography acquisition and amplification minisize sensor includes an electromyographic signal acquisition electrode, a signal processing module, an analog-to-digital conversion module, a modulation emission module, and a display module, the electromyographic signal acquisition electrode is electrically connected with the signal processing module, the signal processing module includes a radio frequency interference suppression circuit, a three-stage amplification circuit, a full-wave rectification circuit, and a filter circuit, the signal processing module is electrically connected with the analog-to-digital conversion module, the analog-to-digital conversion module is electrically connected with the modulation emission module, a bluetooth receiver is arranged on the display module, and the display module receives the signals emitted by the modulation emission module through the bluetooth receiver. The electromyography acquisition and amplification minisize sensor can perform muscle rehabilitation training through the combination with the games on a display panel, and increases the interest of muscle training; and moreover, the sensor is free of a connecting wire, and the binding caused by the connection of various leads when a subject is tested can be prevented.