Method for extracting polypeptide from vinasse

A technology of distiller's grains and water-soluble protein, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield of polypeptides, achieve the effect of improving the effect of enzymatic hydrolysis, ensuring the yield, and avoiding bondage

Active Publication Date: 2017-03-08
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Even so, the yields of polypeptides prepared from oil meal in the above prior art are all low

Method used

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  • Method for extracting polypeptide from vinasse

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Experimental program
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Effect test

Embodiment 1

[0059] Step 1. The wet distiller's grains are vacuum freeze-dried and crushed.

[0060] Step two, degreasing. Use organic solvent cleaning (such as acetone) to clean the above-mentioned crushed residue 2-3 times, and then air dry.

[0061] Step 3: Extraction of water-soluble protein. The vinasse is dissolved in an ethanol alcohol aqueous solution with a mass fraction of 80 wt% in lye, the lye is added to maintain the pH at 13, and the solid is settled by centrifugal separation to obtain a filtrate. Then the filtrate was concentrated to 10% of the original volume with a 600-mesh filter membrane, then acid was added to adjust its pH to 6, and a 600-mesh filter membrane was used to filter to obtain solid components; the solid components were dissolved in water, and then Centrifuge and dry the solid phase.

[0062] Step 4. The above solids are added to the fermenter, and the volume ratio of Trichoderma reesei is 1:0.5:3:1 (the number of viable bacteria is 3×10). 10 fu / ml), Bacillus am...

Embodiment 2

[0067] Step 1. The wet distiller's grains are vacuum freeze-dried and crushed.

[0068] Step two, degreasing. Use organic solvent cleaning (such as acetone) to clean the above-mentioned crushed residue 2-3 times, and then air dry.

[0069] Step 3: Extraction of water-soluble protein. The vinasse is dissolved in an ethanol alcohol aqueous solution with a mass fraction of 50 wt% in lye, the lye is added to maintain the pH at 8, and the solid is settled by centrifugal separation to obtain a filtrate. Then the filtrate was concentrated to 5% of the original volume with a 400-mesh filter membrane, then acid was added to adjust its pH to 2, and then filtered with a 40,000-mesh filter membrane to obtain solid components; the solid components were dissolved in water, and then Centrifuge and dry the solid phase.

[0070] Step 4. The above solids are added to the fermentor, and the volume ratio of Trichoderma reesei is 1:0.8:3:1 (the number of viable bacteria is 5×10). 10 fu / ml), Bacillus a...

Embodiment 3

[0075] Step 1. The wet distiller's grains are vacuum freeze-dried and crushed.

[0076] Step two, degreasing. Use organic solvent cleaning (such as acetone) to clean the above-mentioned crushed residue 2-3 times, and then air dry.

[0077] Step 3: Extraction of water-soluble protein. The vinasse is dissolved in an ethanol alcohol aqueous solution with a mass fraction of 60 wt% in lye, the lye is added to maintain the pH at 10, and the solid is settled by centrifugal separation to obtain a filtrate. Then the filtrate was concentrated to 10% of the original volume using a 400-mesh filter membrane, then acid was added to adjust its pH to 2, and a 400-mesh filter membrane was used to filter to obtain solid components; the solid components were dissolved in water, and then Centrifuge and dry the solid phase.

[0078] Step 4. The above solids are added to the fermentor, and the volume ratio of Trichoderma reesei is 1:0.8:3:2 (the number of viable bacteria is 5×10). 10 fu / ml), Bacillus a...

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Abstract

The invention discloses a method for extracting polypeptide from vinasse. The method includes: (1) degreasing the vinasse; (2) extracting water-soluble protein; (3) subjecting the degreased vinasse to fermentation by the aid of mixed bacterium of Trichoderma reesei, bacillus amyloliquefaciens, bacillus subtilis and Pasteur bacillus licheniformis; (4) subjecting the extracted water-soluble protein and the processed vinasse obtained from the step (3) to enzymolysis by the aid of protease. According to the method, the vinasse is fermented by the mixed bacterium of the Trichoderma reesei, the bacillus amyloliquefaciens, the bacillus subtilis and the Pasteur bacillus licheniformis before protease enzymolysis, wood fibers, semi-fibers and the like in the vinasse can be degraded into micro molecules like polysaccharides, celluloses and the semi-fibers are prevented from constraining the protein, full contact of the protein and the protease is enlarged, an enzymolysis effect of the protein is improved, and a yield rate is guaranteed.

Description

Technical field [0001] The present invention relates to the technical field of polypeptide extraction, in particular to a method for extracting polypeptides from distiller’s grains. Background technique [0002] Distillers' grains, also known as red grains, distiller's grains, dregs, and so on, are the residues left over from the wine making of rice, wheat, and sorghum. Distillers grains are rich in protein, which is a high source of protein. For a long time, most manufacturers mainly sell wet lees as roughage directly at low prices, with little profit; a few manufacturers directly discharge beer lees, which not only causes serious environmental pollution, but also wastes resources. Therefore, the development and comprehensive utilization of beer lees has become an important task for researchers. [0003] A polypeptide is a compound formed by linking amino acids together by peptide bonds. It is also an intermediate product of protein hydrolysis. Many active substances in the hum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12R1/07C12R1/125C12R1/10C12R1/885
Inventor 郭鹏王轶
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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