Extraction method of Chinese wampee leaf active ingredients and applications thereof in preparation of skin care cream
An extraction method and a technology of active ingredients, which are applied in the field of extracting active ingredients of yellow skin leaves and the preparation of skin creams, can solve problems such as incomplete extraction and insufficient utilization of yellow skin leaf resources, so as to improve the purity and improve human health. Detoxification and defense function, effect of scavenging free radicals
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Embodiment 1
[0028] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:
[0029] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;
[0030] 2) Add 5 times the amount of water to soak the yellow leaf powder obtained in step 1) according to the weight of the yellow leaf powder. At the same time, add cellulase with a weight of 1‰ of the yellow leaf powder to the heating reaction kettle to adjust the pH to 6 -7, the temperature is 40°C, the stirring speed is 35rpm, and the enzymolysis is 4hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 1‰ of yellow skin leaves, adjust the pH to 6-7, the temperature is 40°C, and the stirring speed Enzymolysis at 35 rpm for 4 hours; after enzymolysis with the compound protease, tre...
Embodiment 2
[0040] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:
[0041] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;
[0042] 2) Add 5-10 times the amount of water to soak the yellow leaf powder obtained in step 1) according to the weight of the yellow leaf powder, and at the same time add cellulase with a weight of 3‰ of the yellow leaf powder in the heating reaction kettle to adjust the pH 6-7, the temperature is 45°C, the stirring speed is 30rpm, and the enzymolysis is 3hr; after the cellulase enzymolysis, add the compound protease with a weight of 3‰ of yellow skin leaves, adjust the pH to 6-7, and the temperature is 45°C, The stirring speed is 30rpm, and the enzymolysis is 3hr; after enzymolysis with compound protease, treat at 95°C...
Embodiment 3
[0052] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:
[0053] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;
[0054] 2) According to the weight of the wampeskin leaf powder, add 10 times the amount of water to soak the wampeskin leaf powder obtained in step 1), and at the same time add cellulase with a weight of 5‰ of the wampeskin leaf powder in the heating reaction kettle, and adjust the pH to 6 -7, the temperature is 50°C, the stirring speed is 20rpm, and the enzymolysis is 2hr; after the enzymolysis of cellulase, add the compound protease with a weight of 5‰ of yellow skin leaves, adjust the pH to 6-7, the temperature is 50°C, and the stirring speed Enzymolysis at 20 rpm for 2 hours; after enzymolysis with the compound prote...
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