Extraction method of Chinese wampee leaf active ingredients and applications thereof in preparation of skin care cream

An extraction method and a technology of active ingredients, which are applied in the field of extracting active ingredients of yellow skin leaves and the preparation of skin creams, can solve problems such as incomplete extraction and insufficient utilization of yellow skin leaf resources, so as to improve the purity and improve human health. Detoxification and defense function, effect of scavenging free radicals

Inactive Publication Date: 2017-03-15
黄文庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The above-mentioned published literature discusses the extraction of active ingredients of wampum leaves from different angles. The patent application number 201510116170.0 is to add crushed wampee leaves to the two-phase system, ultrasonically for a certain period of time, then filter wampee leaves, and place the filtrate in Leave it still in a separatory funnel, separate the liquid to take the upper layer solution, and obtain the total flavonoids of wampee leaves; the patent application number is 201310247660.5 is to extract the wampee fruit powder with ethanol under reflux, and extract the clear liquid with n-butane, and then vacuum Dried into an extract to obtain the extract of japonica root; the journal titled the process research of macroporous adsorption resin purification of total flavonoids of japonica root is to use macroporous resin to purify the total flavonoids of jasmine root; the above three different Extraction methods are used to obtain the effective components of Lycium barbae for different purposes, but some methods are not completely extracted, which makes the resources of yellow bark leaves not fully utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:

[0029] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;

[0030] 2) Add 5 times the amount of water to soak the yellow leaf powder obtained in step 1) according to the weight of the yellow leaf powder. At the same time, add cellulase with a weight of 1‰ of the yellow leaf powder to the heating reaction kettle to adjust the pH to 6 -7, the temperature is 40°C, the stirring speed is 35rpm, and the enzymolysis is 4hr; after the cellulase enzymatic hydrolysis, add the compound protease with the weight of 1‰ of yellow skin leaves, adjust the pH to 6-7, the temperature is 40°C, and the stirring speed Enzymolysis at 35 rpm for 4 hours; after enzymolysis with the compound protease, tre...

Embodiment 2

[0040] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:

[0041] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;

[0042] 2) Add 5-10 times the amount of water to soak the yellow leaf powder obtained in step 1) according to the weight of the yellow leaf powder, and at the same time add cellulase with a weight of 3‰ of the yellow leaf powder in the heating reaction kettle to adjust the pH 6-7, the temperature is 45°C, the stirring speed is 30rpm, and the enzymolysis is 3hr; after the cellulase enzymolysis, add the compound protease with a weight of 3‰ of yellow skin leaves, adjust the pH to 6-7, and the temperature is 45°C, The stirring speed is 30rpm, and the enzymolysis is 3hr; after enzymolysis with compound protease, treat at 95°C...

Embodiment 3

[0052] The extracting method of the active ingredient of leaves of yellow bark comprises the following steps:

[0053] 1) First remove the soil and weeds of the yellow skin leaves, wash the yellow skin leaves with tap water, dry them in the shade, and then use a pulverizer to crush the yellow skin leaves to 200-300 mesh to obtain the yellow skin leaf powder;

[0054] 2) According to the weight of the wampeskin leaf powder, add 10 times the amount of water to soak the wampeskin leaf powder obtained in step 1), and at the same time add cellulase with a weight of 5‰ of the wampeskin leaf powder in the heating reaction kettle, and adjust the pH to 6 -7, the temperature is 50°C, the stirring speed is 20rpm, and the enzymolysis is 2hr; after the enzymolysis of cellulase, add the compound protease with a weight of 5‰ of yellow skin leaves, adjust the pH to 6-7, the temperature is 50°C, and the stirring speed Enzymolysis at 20 rpm for 2 hours; after enzymolysis with the compound prote...

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PUM

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Abstract

The invention discloses an extraction method of Chinese wampee leaf active ingredients and applications thereof in preparation of a skin care cream. Cellulase and compound protease are applied to loosen and break plant cell walls, reduce mass transfer resistance and accelerate the release of active ingredients, namely flavonoid substances and polyphenols; the slightly alkaline small-molecular water is used for extracting the residual active ingredient from Chinese wampee leaves; the Chinese wampee leaf extract prepared by utilizing the modern bioengineering technology of micro-filtering membrane purification extracting liquid and the like is rich in antioxidant ingredients and antibacterial and anti-inflammatory ingredients of polyphenols, flavones and the like, and the extraction and separation yield is high; and the Chinese wampee leaf active ingredients can be used for preparing skin care cream.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting active ingredients from wampum leaves and its application for preparing skin cream. Background technique [0002] Huangpi, [Latin scientific name] Clausena lansium (Lour.) Skeels [Also known as] yellow bullet, yellow marble, yellow section; Rutaceae, small tree, up to 12 meters high. The leaflets are ovate or ovate-elliptic, asymmetrical on both sides, and the panicles are terminal; the buds are spherical, the calyx lobes are broadly ovate, the petals are oblong, the filaments are linear, the fruit is light yellow to dark yellow, the flesh is milky white, translucent , 1-4 seeds; flowering in April-May, fruiting in July-August. Yellow skin is native to southern China and has a history of more than 1,500 years in China. The flower and fruit period in Hainan, China is 1-2 months earlier. [0003] Yellow skin is one of the fruits in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/9794A61K8/9789A61K8/67A61K8/92A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/988A61K8/678A61K8/922A61K8/97A61K8/986A61K2800/805A61Q19/00A61Q19/02A61Q19/08
Inventor 黄文庆
Owner 黄文庆
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