Preparation and Quality Standard Detection Method of Cordyceps Qishen Capsules

Abstract

The invention belongs to the technical field of traditional Chinese herbal medicine extraction and discloses a preparation and quality standard detection method of cordyceps sinensis, astragalus membranaceus and salvia miltiorrhiza capsules. The method comprises the following steps: 1) preparing cordyceps sinensis fine powder; 2) preparing an astragalus membranaceus extractum; 3) preparing a salvia miltiorrhiza and safflower extractum; 4) preparing a spina date seed extractum; and 5) mixing uniformly, drying and pelleting. According to the preparation method provided by the invention, the effective components of the raw materials can be extracted completely, waste of the raw materials is avoided and the medicine effect is more excellent than that of an existing capsule preparation.

Description

technical field

[0001] The invention belongs to the technical field of Chinese herbal medicine extraction, and in particular relates to the preparation of Cordyceps Qishen Capsules and a quality standard detection method. Background technique

[0002] Cordyceps Qishen Capsules are mainly prepared from Cordyceps sinensis, Astragalus root, Salvia miltiorrhiza, safflower and wild jujube kernels. It has the functions of nourishing the lungs and kidneys, promoting blood circulation and removing blood stasis. It is suitable for syndromes such as lung and kidney qi deficiency and blood stasis caused by chronic nephritis patients. Cordyceps Qishen Capsule is an effective drug for treating chronic nephritis. Its raw material composition is relatively simple, its effect is good, and its toxic and side effects are small, so it is favored by patients. The applicant found in the pharmaceutical process that the preparation method of the drug is relatively simple, and the same extraction ...

Examples

Embodiment 1

[0021] The preparation method of Cordyceps Qishen Capsules comprises the steps of:

[0022] 1) Take 140g of Cordyceps sinensis, crush it into fine powder of Cordyceps sinensis;

[0023] 2) Take 1400g of astragalus, crush it, pass through a 200-mesh sieve, put it in a container, add twice the weight of 85% (v / v) ethanol, stir and extract at 200rpm, control the microwave power to 600W during the extraction process, and the extraction time is 50min Then place it at 4°C for 12 hours, filter, and filter the residue for later use; the filtrate is evaporated under reduced pressure to recover ethanol, and the filtrate is concentrated to extract A with a density of 1.2g / ml; the content of astragaloside IV is 1.27mg by high performance liquid chromatography / ml;

[0024] Add twice the weight of water to the filter residue, stir well, then add 0.5wt% neutral protease (200,000 U / g), enzymatically hydrolyze at 37°C for 120min, then boil for 5min, then add anhydrous Ethanol, stirred at 30...

Embodiment 2

[0038] Cordyceps Qishen Capsule GPM workshop prescription and quality standard test of the present invention are shown in Table 1-2:

[0039] Table 1

[0040] name Dosage kg storage requirements Cordyceps sinensis 67.2 Store in a cool and dry place, away from moths Astragalus 672 Store in a ventilated and dry place, moisture-proof, moth-proof Danshen 268.8 keep in a dry place safflower 268.8 Store in a cool dry place, moisture-proof, moth-proof Suanzaoren 134.4 Store in a cool and dry place, away from moths starch Appropriate amount Keep tightly closed and store in a dry place. empty capsule 480,000 tablets Sealed and stored at a temperature of 10-25°C and a relative humidity of 35-65%.

[0041] Table 2

[0042]

Embodiment 3

[0044] Animal Toxicity Test

[0045] 40 healthy Kunming strain mice, half male and half female, body weight 18.3±1.9g, 40 mice were randomly divided into two groups, each group was half male and half male, of which 20 were the control group and fed with normal water; the other 20 Only mouse is given the capsule preparation prepared by embodiment 1, dosage is 200mg / kg, three times every day, application mouse carries out toxicity experiment and shows: compare with matched group, after administration, mouse does not see significant difference, experiment observes continuously for two weeks, The general condition, food intake, drinking water and weight gain of the mice were normal. On the day of administration and within two weeks after administration, no animal died, suggesting that the drug has low toxicity and is safe for clinical use.

technical field

[0001] The invention belongs to the technical field of Chinese herbal medicine extraction, and in particular relates to the preparation of Cordyceps Qishen Capsules and a quality standard detection method. Background technique

[0002] Cordyceps Qishen Capsules are mainly prepared from Cordyceps sinensis, Astragalus root, Salvia miltiorrhiza, safflower and wild jujube kernels. It has the functions of nourishing the lungs and kidneys, promoting blood circulation and removing blood stasis. It is suitable for syndromes such as lung and kidney qi deficiency and blood stasis caused by chronic nephritis patients. Cordyceps Qishen Capsule is an effective drug for treating chronic nephritis. Its raw material composition is relatively simple, its effect is good, and its toxic and side effects are small, so it is favored by patients. The applicant found in the pharmaceutical process that the preparation method of the drug is relatively simple, and the same extraction ...

Examples

Embodiment 1

[0021] The preparation method of Cordyceps Qishen Capsules comprises the steps of:

[0022] 1) Take 140g of Cordyceps sinensis, crush it into fine powder of Cordyceps sinensis;

[0023] 2) Take 1400g of astragalus, crush it, pass through a 200-mesh sieve, put it in a container, add twice the weight of 85% (v / v) ethanol, stir and extract at 200rpm, control the microwave power to 600W during the extraction process, and the extraction time is 50min Then place it at 4°C for 12 hours, filter, and filter the residue for later use; the filtrate is evaporated under reduced pressure to recover ethanol, and the filtrate is concentrated to extract A with a density of 1.2g / ml; the content of astragaloside IV is 1.27mg by high performance liquid chromatography / ml;

[0024] Add twice the weight of water to the filter residue, stir well, then add 0.5wt% neutral protease (200,000 U / g), enzymatically hydrolyze at 37°C for 120min, then boil for 5min, then add anhydrous Ethanol, stirred at 30...

Embodiment 2

[0038] Cordyceps Qishen Capsule GPM workshop prescription and quality standard test of the present invention are shown in Table 1-2:

[0039] Table 1

[0040] name Dosage kg storage requirements Cordyceps sinensis 67.2 Store in a cool and dry place, away from moths Astragalus 672 Store in a ventilated and dry place, moisture-proof, moth-proof Danshen 268.8 keep in a dry place safflower 268.8 Store in a cool dry place, moisture-proof, moth-proof Suanzaoren 134.4 Store in a cool and dry place, away from moths starch Appropriate amount Keep tightly closed and store in a dry place. empty capsule 480,000 tablets Sealed and stored at a temperature of 10-25°C and a relative humidity of 35-65%.

[0041] Table 2

[0042]

Embodiment 3

[0044] Animal Toxicity Test

[0045] 40 healthy Kunming strain mice, half male and half female, body weight 18.3±1.9g, 40 mice were randomly divided into two groups, each group was half male and half male, of which 20 were the control group and fed with normal water; the other 20 Only mouse is given the capsule preparation prepared by embodiment 1, dosage is 200mg / kg, three times every day, application mouse carries out toxicity experiment and shows: compare with matched group, after administration, mouse does not see significant difference, experiment observes continuously for two weeks, The general condition, food intake, drinking water and weight gain of the mice were normal. On the day of administration and within two weeks after administration, no animal died, suggesting that the drug has low toxicity and is safe for clinical use.

Images