Polysaccharide of organic black fungus and preparation method of particle thereof
A black fungus polysaccharide and black fungus technology are applied in the field of polysaccharide preparation, can solve problems such as consumption, and achieve the effects of high polysaccharide purity, low cost and less organic solvent
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preparation example Construction
[0048] Provide a kind of preparation method of organic black fungus polysaccharide, it is characterized in that: this method comprises the steps:
[0049] Step 1: Take organic black fungus fruiting bodies, dry and pulverize to obtain organic black fungus dry powder;
[0050] Step 2: Mix the dry organic black fungus powder with water and infiltrate it to obtain the extract;
[0051] Step 3: Microwave and ultrasonic extraction are performed on the extract in step 2, and the supernatant is taken for later use;
[0052] Step 4: Take the supernatant in step 3, remove protein and decolorize;
[0053] Step 5: adding ethanol to the product in step 4 for alcohol precipitation, filtering and washing the crude polysaccharide;
[0054] Step 6: Purify the crude polysaccharide in step 5 by high-speed countercurrent chromatography to obtain organic black fungus polysaccharide.
[0055] As mentioned above, organic black fungus is a kind of organic food; organic food (Organic Food) is also ...
Embodiment 1
[0114] 1. Take organic black fungus fruiting bodies, dry and pulverize, and pass through an 80-mesh sieve to obtain organic black fungus dry powder;
[0115] 2. Mix organic black fungus dry powder with distilled water at a mass ratio of 1:10 to obtain an extract;
[0116] 3. The extract in step 2 is subjected to microwave and ultrasonic extraction for synergistic extraction, the microwave power is 500W, the time is 2 minutes, the ultrasonic power is 700W, the time is 5 minutes, filtered through filter cloth, and the supernatant is taken for use;
[0117] 4. Take the supernatant in step 3, add chloroform: n-butanol = 5:1 (V / V), stir and mix well, take the supernatant after centrifugation; add activated carbon, filter after boiling, take the upper Clear liquid is obtained;
[0118] 5. Add the product in step 4 to ethanol for alcohol precipitation, the alcohol precipitation time is 5 hours, filter, wash with ethanol, and polysaccharide crude product;
[0119] 6. Use high-speed ...
Embodiment 2
[0122] 1. Take organic black fungus fruiting bodies, dry and pulverize, and pass through a 50-mesh sieve to obtain organic black fungus dry powder;
[0123] 2. Mix organic black fungus dry powder with distilled water at a mass ratio of 1:20 to obtain an extract;
[0124] 3. The extract in step 2 is subjected to microwave and ultrasonic extraction for synergistic extraction, the microwave power is 650W, the time is 3 minutes, the ultrasonic power is 900W, the time is 2 minutes, filtered through filter cloth, and the supernatant is taken for use;
[0125] 4. Take the supernatant in step 3, add chloroform: n-butanol = 5:1 (V / V), stir and mix well, take the supernatant after centrifugation; add activated carbon, filter after boiling, take the upper Clear liquid is obtained;
[0126] 5. Add the product in step 4 to ethanol for alcohol precipitation. The alcohol precipitation time is 8 hours, filter and wash with ethanol to obtain crude polysaccharide;
[0127] 6. Use high-speed c...
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