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Preparation method of sensor for detecting vanillyl mandelic acid and application of sensor

A sensor and isoorientin technology, applied in the field of electrochemical analysis, can solve the problems of spectrophotometry, such as susceptibility to matrix interference, poor detection accuracy, and expensive instruments, so as to achieve short analysis time of samples and meet the requirements of rapid detection and detection The effect of low cost

Active Publication Date: 2017-03-15
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, liquid mass spectrometry or spectrophotometry after extraction is mainly used for clinical determination. Liquid mass spectrometry equipment is expensive, which is not conducive to popularization. Spectrophotometry is easily interfered by matrix, and the detection accuracy is not good.

Method used

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  • Preparation method of sensor for detecting vanillyl mandelic acid and application of sensor
  • Preparation method of sensor for detecting vanillyl mandelic acid and application of sensor
  • Preparation method of sensor for detecting vanillyl mandelic acid and application of sensor

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A preparation method of an electrochemical sensor, comprising the following steps:

[0028] Drop-coat 2µl 5mg / ml isoorientin solution on the surface of the activated electrode, let it dry naturally, put it in a phosphate buffer solution with a concentration of 0.05mol / L and pH=2, and keep it at -0.2~+1.2 Within the range of V potential, cyclically scan 40 times at a scan rate of 50 mV / s, cover the electrode cap and save it for later use to obtain the electrochemical sensor provided by the present invention.

Embodiment 2

[0030] A preparation method of an electrochemical sensor, comprising the following steps:

[0031] Drop-coat 10µl of 0.5mg / ml isoorientin solution on the surface of the activated electrode, let it dry naturally, put it in a phosphate buffer solution with a concentration of 0.05mol / L and pH=2, and store it at -0.2~+ Within the potential range of 1.2V, scan 20 times at a scan rate of 100mV / s, cover the electrode cap and save it for later use, and obtain the electrochemical sensor provided by the present invention.

Embodiment 3

[0033] A preparation method of an electrochemical sensor, comprising the following steps:

[0034] Drop-coat 10µl 0.5mg / ml isoorientin solution on the surface of the activated electrode, let it dry naturally, put it in a phosphate buffer solution with a concentration of 0.05mol / L and pH=3, and store it at -0.2~+ Within the potential range of 1.2V, scan 40 times at a scanning speed of 50mV / s, cover the electrode cap and save it for later use, and obtain the electrochemical sensor provided by the present invention.

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Abstract

The invention discloses a preparation method of a sensor for detecting vanillyl mandelic acid and application of the sensor. The electrochemical sensor comprises a glassy carbon electrode, and a sensing film formed by isoorientin is dropwise coated and electrodeposited on the surface of the glassy carbon electrode. The preparation method includes: 1), dropwise coating the surface of the glassy carbon electrode which is already activated with isoorientin, and naturally airing; 2), putting the glassy carbon electrode obtained in the step 1) in a phosphate buffering solution for electrochemical polymerization to obtain the sensor. The sensor can avoid interference of a urine matrix, realizes detection of the vanillyl mandelic acid and can be successfully used for sensing detection of the vanillyl mandelic acid in human urine.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis, and in particular relates to a preparation method and application of an electrochemical sensor. Background technique [0002] Pheochromocytoma is a rare neuroendocrine tumor, which is divided into adrenal pheochromocytoma and ectopic pheochromocytoma (paraganglioma). It can cause the triad of "palpitations, profuse sweating, headache," and other symptoms include anxiety, polyuria, polydipsia, weight loss, chest pain, and abdominal pain. Since there are no specific typical clinical manifestations, and during the course of diagnosis and treatment, patients often When two of the triads appear, one of them is easily overlooked, and when three of them appear, the symptoms are often severe. Long-term catecholamine secretion, resulting in paroxysmal or persistent hypertension can cause serious brain, heart, kidney and blood vessel damage, and even death. If the diagnosis can be made in time, mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 姚宏王盼盼史培颖黄建勇陈嘉璇林新华
Owner FUJIAN MEDICAL UNIV
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