Arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato

Abstract

The invention discloses arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato. 16SrRNA of the arthrobacter sp. has a nucleotide sequence shown as SEQ ID No.1. The invention also discloses a screening method of the arthrobacter sp. as well as preparation methods of a tomato bacterial wilt antagonistic fungicide and a tomato bacterial wilt antagonistic breeding medium. According to the invention, by separating and screening tomato bacterial wilt antagonistic bacteria and by conducting control effect experiments, a strain, which has an antagonistic action on the tomato bacterial wilt, is separated and screened out; firstly, through culture and propagation, the finished fungicide product is prepared, and then, mushroom residues, obtained from edible fungi cultivation, are fermented and rotten and are mixed with other substrates according to a certain proportion, so that the breeding medium is prepared; the biocontrol fungicide and the breeding medium are organically combined; the arthrobacter sp. has the advantages of being rich in available resources, low in cost, capable of preventing secondary pollution to environment when used for controlling the bacterial wilt and the like; therefore, an effective way is provided for solving the soil-borne disease, namely the bacterial wilt of tomato.

Description

technical field

[0001] The invention relates to the technical field of tomato bacterial wilt biocontrol, in particular to an Arthrobacter sp., an anti-tomato bacterial wilt bacterial agent, an antagonistic tomato bacterial wilt seedling cultivation substrate and an application in preventing and treating tomato bacterial wilt. Background technique

[0002] Bacterial wilt of tomato is a bacterial soil-borne disease caused by Ralstonia solanacearum, mostly in tropical and subtropical countries and regions, such as: South America, Japan, India, The Philippines, my country's Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, Chongqing and other places. The disease can cause large areas of wilting and death of plants. The incidence rate of general fields is 10% to 15%, and the incidence rate of severe disease fields is as high as 80% to 98.5%, resulting in a serious decline in tomato yield and major economic losses. R. solanacearum is a soil-inhabitant fungus that can survive i...

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