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Arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato

A technology for tomato bacterial wilt and Arthrobacter, applied in antagonizing tomato bacterial wilt seedling substrate, Arthrobacter, preventing and treating tomato bacterial wilt, in the field of antagonizing tomato bacterial wilt, and can solve the problem of resistance to chemical pesticides , rising production costs, lack of chemical agents for tomato bacterial wilt, etc., to achieve the effect of abundant available resources and low cost

Active Publication Date: 2017-03-22
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is a lack of effective chemical agents against tomato bacterial wilt, and R. solanacearum is prone to resistance to chemical pesticides, and the extensive use of chemical pesticides will cause environmental pollution and increase production costs

Method used

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  • Arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato
  • Arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato
  • Arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the acquisition of Arthrobacter sp. SJN-5 bacterial strain

[0039] This example provides a bacterial strain for the prevention and treatment of tomato bacterial wilt by using pathogenic bacteria antagonistic microorganisms, specifically Arthrobacter sp. The soil samples of continuous cropping tomato fields were collected in different areas such as Jixi, Jixi, etc., and more than 100 strains of various bacteria were directionally isolated from the bacterial culture medium by the dilution separation method, and the strains were purified. Arthrobacter SJN-5, which antagonizes tomato R. solanacearum, was screened out by culturing at 30°C and inoculating on various seedling substrates.

[0040] 1 medium

[0041] Casein peptone agar medium: glucose 5.0g, casein hydrolyzate 1.0g, bacto-grade peptone 10.0g, agar 15.0-20.0g, distilled water 1000ml, pH value 7.0-7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0042] 2 Test steps

[0043...

Embodiment 2

[0060] Embodiment 2: Plate antagonism test of isolated bacteria to R. solanacearum of tomato bacterial wilt

[0061] 1. Medium

[0062] 1.1 Solid medium

[0063] Preparation of culture medium for R. solanacearum: 0.2g potassium dihydrogen phosphate, 0.8g dipotassium hydrogen phosphate, 5.0g yeast extract, 1.0g casein hydrolyzate, 5.0g glucose, 15.0g agar, 1000ml distilled water, pH 7.0~ 7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0064] The preparation of casein peptone agar medium: with the casein peptone agar medium among the embodiment 1.

[0065] 1.2 Liquid medium

[0066] Casein peptone agar medium can be used without adding agar, and both Arthrobacter and pathogenic bacteria of solanacearum can grow on the liquid medium.

[0067] 2. Test steps

[0068] 2.1 Culture medium preparation

[0069] Prepare Ralstonia solanacearum culture medium and liquid culture medium respectively according to above-mentioned formula, prepare pathogenic bact...

Embodiment 3

[0084] Embodiment 3: the preparation of the antagonistic antibiotic of bacterial strain SJN-5

[0085] The microbial bacterial agent capable of antagonizing tomato bacterial wilt in this embodiment is produced by culturing and propagating Arthrobacter sp. SJN-5 strain.

[0086] The specific operation process is as follows: Arthrobacter SJN-5 is based on casein peptone medium, adding inorganic salt calcium 0.9-1.5g / l, magnesium 0.5-1.3g / l, phosphorus 0.8-2.5g / l to carry out liquid shake flask fermentation, 25 Cultivate at ~35°C with a rotational speed of 120-220rpm. Use this as bacterial classification inoculation liquid fermenter to make liquid parent species, liquid fermenter raw material is bran, vegetable cake and bean cake powder by weight percentage 1%~5%: 0~3%: 0~3%: 0~3 %) and a small amount of inorganic salts (calcium 0.9~1.5g / l, magnesium 0.5~1.3g / l, phosphorus 0.8~2.5g / l), the amount of water added is 99~89%, after autoclaving, the liquid in the fermentation tank W...

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Abstract

The invention discloses arthrobacter sp. and application thereof in aspect of biological control of bacterial wilt of tomato. 16SrRNA of the arthrobacter sp. has a nucleotide sequence shown as SEQ ID No.1. The invention also discloses a screening method of the arthrobacter sp. as well as preparation methods of a tomato bacterial wilt antagonistic fungicide and a tomato bacterial wilt antagonistic breeding medium. According to the invention, by separating and screening tomato bacterial wilt antagonistic bacteria and by conducting control effect experiments, a strain, which has an antagonistic action on the tomato bacterial wilt, is separated and screened out; firstly, through culture and propagation, the finished fungicide product is prepared, and then, mushroom residues, obtained from edible fungi cultivation, are fermented and rotten and are mixed with other substrates according to a certain proportion, so that the breeding medium is prepared; the biocontrol fungicide and the breeding medium are organically combined; the arthrobacter sp. has the advantages of being rich in available resources, low in cost, capable of preventing secondary pollution to environment when used for controlling the bacterial wilt and the like; therefore, an effective way is provided for solving the soil-borne disease, namely the bacterial wilt of tomato.

Description

technical field [0001] The invention relates to the technical field of tomato bacterial wilt biocontrol, in particular to an Arthrobacter sp., an anti-tomato bacterial wilt bacterial agent, an antagonistic tomato bacterial wilt seedling cultivation substrate and an application in preventing and treating tomato bacterial wilt. Background technique [0002] Bacterial wilt of tomato is a bacterial soil-borne disease caused by Ralstonia solanacearum, mostly in tropical and subtropical countries and regions, such as: South America, Japan, India, The Philippines, my country's Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, Chongqing and other places. The disease can cause large areas of wilting and death of plants. The incidence rate of general fields is 10% to 15%, and the incidence rate of severe disease fields is as high as 80% to 98.5%, resulting in a serious decline in tomato yield and major economic losses. R. solanacearum is a soil-inhabitant fungus that can survive i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/02A01P1/00C05G3/00C12R1/06
CPCA01N63/10C05C9/00C05D9/00C05G3/00C12N1/02C12N1/205C12R2001/06C05D3/00C05D3/02C05F5/00C05F5/002C05F11/08C05F17/00C05F9/04C05F11/02
Inventor 张建王朋成方凌王艳江海坤张其安严从生
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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