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A kind of Arthrobacter and its application in biological control of tomato bacterial wilt

A technology for tomato bacterial wilt disease and tomato bacterial wilt fungus, which is applied to the field of tomato bacterial wilt disease biocontrol, can solve the problems of rising production costs, easy generation of chemical pesticide resistance, and lack of chemical agents for tomato bacterial wilt disease, and achieves low cost. , the effect of abundant resources available

Active Publication Date: 2019-10-08
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is a lack of effective chemical agents against tomato bacterial wilt, and R. solanacearum is prone to resistance to chemical pesticides, and the extensive use of chemical pesticides will cause environmental pollution and increase production costs

Method used

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  • A kind of Arthrobacter and its application in biological control of tomato bacterial wilt
  • A kind of Arthrobacter and its application in biological control of tomato bacterial wilt
  • A kind of Arthrobacter and its application in biological control of tomato bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the acquisition of Arthrobacter sp. SJN-5 bacterial strain

[0039] This example provides a bacterial strain for the prevention and treatment of tomato bacterial wilt by using pathogenic bacteria antagonistic microorganisms, specifically Arthrobacter sp. The soil samples of continuous cropping tomato fields were collected in different areas such as Jixi, Jixi, etc., and more than 100 strains of various bacteria were directionally isolated from the bacterial culture medium by the dilution separation method, and the strains were purified. Arthrobacter SJN-5, which antagonizes tomato R. solanacearum, was screened out by culturing at 30°C and inoculating on various seedling substrates.

[0040] 1 medium

[0041] Casein peptone agar medium: glucose 5.0g, casein hydrolyzate 1.0g, bacto-grade peptone 10.0g, agar 15.0-20.0g, distilled water 1000ml, pH value 7.0-7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0042] 2 Test steps

[0043...

Embodiment 2

[0060] Embodiment 2: Plate antagonism test of isolated bacteria to R. solanacearum of tomato bacterial wilt

[0061] 1. Medium

[0062] 1.1 Solid medium

[0063] Preparation of culture medium for R. solanacearum: 0.2g potassium dihydrogen phosphate, 0.8g dipotassium hydrogen phosphate, 5.0g yeast extract, 1.0g casein hydrolyzate, 5.0g glucose, 15.0g agar, 1000ml distilled water, pH 7.0~ 7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0064] The preparation of casein peptone agar medium: with the casein peptone agar medium among the embodiment 1.

[0065] 1.2 Liquid medium

[0066] Casein peptone agar medium can be used without adding agar, and both Arthrobacter and pathogenic bacteria of solanacearum can grow on the liquid medium.

[0067] 2. Test steps

[0068] 2.1 Culture medium preparation

[0069] Prepare Ralstonia solanacearum culture medium and liquid culture medium respectively according to above-mentioned formula, prepare pathogenic bact...

Embodiment 3

[0084] Embodiment 3: the preparation of the antagonistic antibiotic of bacterial strain SJN-5

[0085] The microbial bacterial agent capable of antagonizing tomato bacterial wilt in this embodiment is produced by culturing and propagating Arthrobacter sp. SJN-5 strain.

[0086] The specific operation process is as follows: Arthrobacter SJN-5 is based on casein peptone medium, adding inorganic salt calcium 0.9-1.5g / l, magnesium 0.5-1.3g / l, phosphorus 0.8-2.5g / l to carry out liquid shake flask fermentation, 25 Cultivate at ~35°C with a rotational speed of 120-220rpm. Use this as bacterial classification inoculation liquid fermenter to make liquid parent species, liquid fermenter raw material is bran, vegetable cake and bean cake powder by weight percentage 1%~5%: 0~3%: 0~3%: 0~3 %) and a small amount of inorganic salts (calcium 0.9~1.5g / l, magnesium 0.5~1.3g / l, phosphorus 0.8~2.5g / l), the amount of water added is 99~89%, after autoclaving, the liquid in the fermentation tank W...

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Abstract

The invention discloses an Arthrobacter and its application in biological control of tomato bacterial wilt. The 16SrRNA of Arthrobacter has the nucleotide sequence shown in SEQ ID No.1. The invention also discloses a screening method for Arthrobacter and a preparation method for antagonizing tomato bacterial wilt bacterial agent and antagonizing tomato bacterial wilt seedling growth matrix. The present invention isolates and screens the antagonistic bacteria against tomato bacterial wilt and tests its control effect, isolates and screens out the bacterial strains that have antagonism to tomato bacterial wilt, first cultures and propagates them, and makes the bacterial agent product, and cultivates them with edible fungi. After the mushroom dregs are fermented and decomposed, they are mixed with other substrates in a certain proportion to form a seedling substrate, and the biocontrol agent is directly organically combined with the seedling substrate. It has abundant available resources and low cost. Using it to control R. solanacearum can Avoiding secondary pollution of the environment and many other advantages is an effective way to solve the soil-borne disease tomato bacterial wilt.

Description

technical field [0001] The invention relates to the technical field of tomato bacterial wilt biocontrol, in particular to an Arthrobacter sp., an anti-tomato bacterial wilt bacterial agent, an antagonistic tomato bacterial wilt seedling cultivation substrate and an application in preventing and treating tomato bacterial wilt. Background technique [0002] Bacterial wilt of tomato is a bacterial soil-borne disease caused by Ralstonia solanacearum, mostly in tropical and subtropical countries and regions, such as: South America, Japan, India, The Philippines, my country's Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, Chongqing and other places. The disease can cause large areas of wilting and death of plants. The incidence rate of general fields is 10% to 15%, and the incidence rate of severe disease fields is as high as 80% to 98.5%, resulting in a serious decline in tomato yield and major economic losses. R. solanacearum is a soil-inhabitant fungus that can survive i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/02A01P1/00C05G3/00C12R1/06
CPCA01N63/10C05C9/00C05D9/00C05G3/00C12N1/02C12N1/205C12R2001/06C05D3/00C05D3/02C05F5/00C05F5/002C05F11/08C05F17/00C05F9/04C05F11/02
Inventor 张建王朋成方凌王艳江海坤张其安严从生
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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