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A strain antagonizing melon fruit blotch and its application

A technology of bacterial strains and bacterial counts, applied in the field of biological control, can solve problems such as rising production costs, lack of chemical agents for melon fruit spot disease, and environmental pollution

Active Publication Date: 2021-09-10
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is a lack of effective chemical agents for the fruit spot disease of melon, and the fruit spot bacteria are prone to resistance to chemical pesticides, and the large-scale use of chemical pesticides will cause environmental pollution and increase production costs.

Method used

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  • A strain antagonizing melon fruit blotch and its application
  • A strain antagonizing melon fruit blotch and its application
  • A strain antagonizing melon fruit blotch and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preliminary screening of Burkholderia sp. strains

[0050] 1 medium

[0051] Casein peptone agar medium: glucose 5.0g, casein hydrolyzate 1.0g, bacto-grade peptone 10.0g, glycerin 5ml, agar 15.0-20.0g, distilled water 1000ml, pH value 7.0-7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0052] 2 test steps

[0053] 2.1 Preparation of medium

[0054] Prepare casein peptone agar medium as described in 1 to prepare bacterial slant medium.

[0055] 2.2 Preparation of soil dilution

[0056] (1) Take by weighing 10g soil sample, add in 90ml sterile water 250ml Erlenmeyer flask, vibrate 15 minutes, obtain 10 -1 The diluted soil solution A;

[0057] (2) Draw diluted soil solution A1ml, add in 9ml sterile water 50ml Erlenmeyer flask, obtain 10 -2 The diluted soil solution B;

[0058] (3) Draw diluted soil solution B1ml, add in 9ml sterile water 50ml Erlenmeyer flask, obtain 10 -3 The diluted soil solution C;

[0059] (4) Draw diluted soil soluti...

Embodiment 2

[0071] Plate Antagonism Test of the Isolated Bacteria to the Pathogen of Melon Fruit Spot Solanum

[0072] 1. Medium

[0073] 1.1 Solid medium

[0074] Preparation of culture medium for melon fruit blight: 0.2g potassium dihydrogen phosphate, 0.8g dipotassium hydrogen phosphate, 5.0g yeast extract, 1.0g casein hydrolyzate, 5.0g glucose, 15.0g agar, 1000ml distilled water, pH 7.0-7.2. Sterilize at 115.0°C for 25 minutes with high-pressure damp heat.

[0075] The preparation of casein peptone agar medium: with the casein peptone agar medium among the embodiment 1.

[0076] 1.2 Liquid medium

[0077] Casein peptone agar medium can be used without adding agar, and both Burkholderia and melon fruit spot pathogen can grow on the liquid medium.

[0078] 2. Test steps

[0079] 2.1 Culture medium preparation

[0080] According to the above-mentioned formula, respectively prepare the culture medium and the liquid culture medium of the melon fruit spot pathogen, and prepare the sl...

Embodiment 3

[0096] Bacteria N01 to prepare biocontrol agent

[0097] (1) Burkholderia N01, liquid culture medium, inorganic salt calcium, magnesium, phosphorus mixed with mass volume ratio 1% carry out liquid shake flask fermentation, 25 ℃ of cultures, rotating speed 120rpm, obtain seed liquid, wherein The number of Burkholderia N01 reached 10 8 cfu / ml;

[0098] (2) Enlarged cultivation: raw material is bran of 1% by weight, inorganic salt calcium, magnesium, phosphorus and water amount 99~89% (5L liquid fermenter), stirs and mixes, obtains mixed solution; After 115 ℃ of autoclaving, When the temperature of the mixed liquid is cooled to below 35°C, inoculate the seed liquid with an inoculum volume of 1% by mass-volume ratio in an aseptic manner, and always maintain a positive pressure environment above 0.01MPa, and cultivate it with ventilation at 35°C for 96 hours until the fermented bacteria count up to 10 8 More than cfu / ml, wherein, in steps (1) and (2), the final concentration of ...

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Abstract

The invention discloses a bacterial strain antagonizing melon fruit blotch and application thereof. It belongs to the technical field of biological control. Including a Burkholderia sp.N01 (Burkholderia sp.N01), the preservation number is CCTCC NO: M2020248, and provides a method for preparing a biocontrol agent and optimizing the seedling substrate by using it in combination with nutritional supplements application. The bacterial strain provided by the invention has the characteristics of well inhibiting the growth of the fruit spot fungus and being tolerant to heavy metal cadmium ions. After cultivating and propagating the antagonistic bacteria of melon fruit spot disease, a biocontrol agent can be made, and then a seedling-raising substrate containing the above-mentioned strains or biocontrol agents can be prepared, and the composition of the seedling-raising substrate can be optimized by adding nutritional supplements, which can effectively reduce the It prevents melon fruit spot disease and meets the nutrient requirements of melon seedling growth.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a strain antagonizing melon fruit spot disease and application thereof. Background technique [0002] Bacterial Fruit Blotch (BFB) of melons is the most important and destructive seed-borne bacterial quarantine disease on watermelons and muskmelons. The pathogen is Acidovorax citrulli. Melon fruit spot disease has occurred in countries such as the United States, Australia, Korea, Japan, Turkey and Thailand. my country's watermelon and melon planting area and output not only rank among the top in the world, but also our country is an important seed production base of melon. Since the disease was first reported in my country in 1998, it has occurred in a large area in my country and is on the rise. It has caused the production of watermelon and melon in the field to decrease or even fail, and has caused huge losses to the melon planting industry. How to effectively red...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01G24/22A01G24/28A01G24/15A01G24/20A01G24/10A01G31/00A01G13/00C12R1/01
CPCA01G13/00A01G31/00A01G24/10A01G24/15A01G24/20A01G24/22A01G24/28A01N63/20C12N1/20C12N1/205C12R2001/01
Inventor 张建王朋成田红梅陶珍许钢王明霞王艳王家嘉朱培蕾赵贵云
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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