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A kind of determination method of biomass in activated sludge embedding gel

A technology of activated sludge and determination method, which is applied in the field of determination of biomass in embedded gel, can solve the problems such as difficult determination of embedded particle biomass, and achieve repeatability, high sensitivity and high repeatability of measurement results Effect

Active Publication Date: 2020-01-17
BEIJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the deficiencies of the above-mentioned technology, the present invention utilizes the ultrasonic method to extract the protein in the embedding particle, then utilizes BCA kit (Beyotime) to measure its protein concentration, determines the biomass in the embedding particle by protein concentration, to solve Difficult to measure biomass in embedded particles

Method used

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  • A kind of determination method of biomass in activated sludge embedding gel
  • A kind of determination method of biomass in activated sludge embedding gel
  • A kind of determination method of biomass in activated sludge embedding gel

Examples

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Embodiment 1

[0030] Utilize the present invention to measure the biomass in the anammox embedding particle, the specific process is as follows:

[0031] Take a certain volume of anaerobic ammonium oxidation sludge with stable treatment effect, according to MLVSS (in mg): 10, 30, 50, 70, 90, 110, 130, 150. Rinse with 50ml of PBS and pour into the first in a triangular beaker. Another 20ml volume of anammox embedding particles was taken, washed with 50ml PBS after crushing, and then poured into the second conical beaker. Add 1ml of lysate to the first conical beaker and the first conical beaker respectively (according to every 10 7 The ratio of each cell to 1ml of cell lysate), and the protein samples in the anammox sludge and embedded particle samples were extracted respectively by ultrasonic waves. Working conditions: ultrasonic (11kHz, 10s×84 cycles, interval 1min, ice bath), oscillation (5×30s, interval 1min, ice bath). Centrifuge the treated suspension at 4°C and 14,000 rpm for 30 mi...

Embodiment 2

[0036] Using the present invention to measure the biomass in the denitrification embedding particles, the specific process is as follows:

[0037] Take a certain volume of denitrification sludge with stable treatment effect, according to MLVSS (in mg): 10, 30, 50, 70, 90, 110, 130, 150. Rinse them with 50ml of PBS and pour them into a triangular beaker . Another 20ml volume of denitrification embedding particles was taken, washed with 50ml PBS after crushing, and then poured into a triangular beaker. Add 1ml of lysate to the first conical beaker and the first conical beaker respectively (according to every 10 7 The ratio of each cell to 1ml of cell lysate), and the protein samples in the denitrification sludge and embedded particle samples were extracted respectively by ultrasonic waves. Working conditions: ultrasonic (11kHz, 10s×84 cycles, interval 1min, ice bath), oscillation (5×30s, interval 1min, ice bath). Centrifuge the treated suspension at 4°C and 14,000 rpm for 30 ...

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Abstract

The invention discloses a method for measuring biomass in embedding gel. The method which measures the protein concentration in the embedding gel to indirectly measure the biomass of the embedding gel includes pretreating a sample, extracting bacterium total protein, measuring protein concentration and processing data and is characterized in that a BCA method is used to measure the protein concentration, the measured protein concentration in active sludge is fitted with MLVSS content to obtain a biomass standard curve, and the measured protein concentration in the embedding gel is substituted into the standard curve to obtain the biomass of the embedding gel. The method is simple to operate, reliable in measuring result, high in repeatability and widely applicable to the measuring of the biomass in embedding particles in the field of sewage treatment.

Description

technical field [0001] The invention belongs to the field of sewage treatment, in particular to a method for measuring biomass in an embedding gel, and further relates to a method for indirectly measuring the biomass by measuring the protein concentration in the embedding gel. Background technique [0002] Embedding and immobilization technology is a new type of microorganism immobilization technology, which uses polyvinyl alcohol (PVA), sodium alginate (SA), water-based polyurethane (WPU) and other materials to embed microorganisms in polymer films or gel cells. In the grid, the grid structure of the carrier can fix the microbial living cells in a specific position without leakage, and at the same time allow the free diffusion of small molecule substrates and reaction metabolites to achieve the purpose of increasing the biomass in the reactor. [0003] The ability of microbial populations to degrade organic matter in biological treatment systems depends on the biomass in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28G01N1/34
CPCG01N1/286G01N1/34G01N21/31G01N2021/3185
Inventor 李军王秀杰卞伟王维奇王思宇
Owner BEIJING UNIV OF TECH
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