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Separation method for phenolic compounds in rubus corchorifolius

The technology of a phenolic compound and a separation method is applied in the field of separation of phenolic compounds in raspberries, which can solve the problem of high difficulty in determination and separation, and achieve the effects of stable and reliable method, easy process control, and simple and effective method.

Inactive Publication Date: 2017-03-22
TONGREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above phenolic substances are biologically active and have anti-tumor and other effects. However, it is difficult to measure their content and separate them, and there are no relevant reports.

Method used

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  • Separation method for phenolic compounds in rubus corchorifolius
  • Separation method for phenolic compounds in rubus corchorifolius
  • Separation method for phenolic compounds in rubus corchorifolius

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Follow the steps below to perform pretreatment before separation of various phenolic compounds in raspberry

[0031] 1) 3g raspberry sample and 15mL acetone were placed in a 50mL centrifuge tube and stirred with a homogenizer at 15000rpm for 80 seconds to obtain a mixture of sample and acetone;

[0032] 2) and the mixture obtained in step 1) was filtered under vacuum, and the residue was washed 3 times with 5 mL of acetone, and the 3 times of filtrates were combined, wherein the filtering operation was carried out using a funnel filter paper, and a layer of glass was covered on the funnel filter paper cotton;

[0033] 3) Use a rotary condensation evaporator to evaporate the filtrate at 35°C until small yellow particles appear and stop evaporating;

[0034] 4) After evaporation, the concentrated sample was dissolved in 5 mL of 2% acetic acid, and passed through the C18Sep-Pak column, wherein, before the C18Sep-Pak column was used, it was activated with 5 ml of methanol,...

Embodiment 2

[0038] Follow the steps below to perform pretreatment before separation of various phenolic compounds in raspberry

[0039] 1) 3g raspberry sample and 15mL acetone were placed in a 50mL centrifuge tube and stirred with a homogenizer at 17500rpm for 1 minute to obtain a mixture of sample and acetone;

[0040] 2) and the mixture obtained in step 1) was filtered under vacuum, and the residue was washed 5 times with 5 mL of acetone, and the 5 times of filtrates were combined, wherein the filtering operation was carried out using a funnel filter paper, and a layer of glass was covered on the funnel filter paper cotton;

[0041] 3) Use a rotary condensation evaporator to evaporate the filtrate at 30°C until small yellow particles appear and stop evaporating;

[0042] 4) After evaporation, the concentrated sample was dissolved in 5 mL of 3% formic acid, and passed through a C18Sep-Pak column, wherein the C18Sep-Pak column was activated with 5 ml of methanol, 6 ml of deionized water,...

Embodiment 3

[0046] Follow the steps below to perform pretreatment before separation of various phenolic compounds in raspberry

[0047] 1) 3g raspberry sample and 15mL acetone were placed in a 50mL centrifuge tube and stirred with a homogenizer at 20000rpm for 40 seconds to obtain a mixture of the sample and acetone;

[0048] 2) and filter the mixture obtained in step (1) under vacuum, and wash the residue 3 times with 5 mL of acetone, and combine the 3 times of filtrate;

[0049] 3) Use a rotary condensation evaporator to evaporate the filtrate at 40°C until small yellow particles appear and stop evaporating;

[0050]4) After evaporation, the concentrated sample was dissolved in 5 mL of 5% formic acid and passed through a C18Sep-Pak column;

[0051] 5) Reactivate anthocyanins and other phenolic compounds with 2 mL of acidified methanol containing 5% formic acid and recover them. All recovered samples are filtered through a 0.45 μm syringe filter, and 10 μL of each sample’s extract is us...

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Abstract

A separation method for phenolic compounds in rubus corchorifolius comprises the following steps: 1) rubus corchorifolius samples are dissolved with a ketone solvent and mixed uniformly; 2) a filtrate is taken after filtering and is concentrated, and the filtrate is diluted with an organic acid; 3) the diluted filtrate is purified by a solid-phase extraction column, and an acidified alcohol solvent is added; 4) HPLC (high-performance liquid chromatography) is adopted for detection and separation of a sample mixture. In the separation method, the rubus corchorifolius pretreatment method is simple and effective; the separation method is implemented via HPLC, the process is easy to control, and the method is stable and reliable. Eight phenolics in the rubus corchorifolius can be obtained with the method, and powerful guidance is provided for detection of the phenolic compounds.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to a method for separating phenolic compounds in raspberries. Background technique [0002] Raspberry (scientific name: Rubus corchorifolius L.f.), also known as raspberry, mountain thrower, milk bubble, seedling bubble, March bubble, April bubble, dragon boat bubble, barley bubble, bubble thorn, thorn gourd, steamed bread spinach, Tall feet, erect shrubs, 1-3 m tall; branches with prickles, pilose when young. Single leaf, ovate to ovate-lanceolate, mostly grows in sunny hillsides, valleys, wastelands, streamsides and wet places in dense bushes, and has not yet been artificially introduced and cultivated. The flowering period is 2-3 months, and the fruiting period is 4-6 months. It has the effects of astringent, essence, kidney, yang and eyesight, sobering up and quenching thirst, resolving phlegm and detoxification, and mainly treating diseases such as kidney deficiency...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张志敏朱祥丁新泉王锐姚琦馥李建新
Owner TONGREN UNIV
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