This invention relates to the field of
traditional Chinese medicine quality testing technology, specifically to an
HPLC fingerprint spectrum of *Caulis Amaranthus urinaria* (also known as *Caulis Amaranthus urinaria*), its construction method, and its application. The construction method includes the following steps: preparation of a
test solution, preparation of a mixed reference solution,
fingerprint spectrum construction, multi-component quantitative analysis, and chemical
pattern recognition. This invention combines HPLC fingerprinting, multi-component quantitative analysis, and chemical
pattern recognition technologies, enabling efficient and accurate characterization of the overall quality of *Caulis Amaranthus urinaria* and precise evaluation of its differences. This provides a basis for the formulation of quality standards,
quality control, selection of superior production areas, and selection of clinical medications for *Caulis Amaranthus urinaria*.