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Kit used for determination of triiodothyronine

A technology of triiodothyronine and protosine, applied in the field of kits, can solve the problems of poor specificity and sensitivity of T3 immunoassay reagents, and achieve the effect of high specificity and sensitivity

Active Publication Date: 2017-03-22
SICHUAN ORIENTER BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a kit for determining triiodothyronine, aiming at solving the problem of poor specificity and sensitivity of T3 immunoassay reagents in the prior art

Method used

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  • Kit used for determination of triiodothyronine
  • Kit used for determination of triiodothyronine
  • Kit used for determination of triiodothyronine

Examples

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Effect test

Embodiment 1

[0026] A kit for the determination of triiodothyronine, comprising goat monoclonal antibody-coated magnetic beads, ANS dissociation agent and enzyme working solution, and the goat monoclonal antibody-coated magnetic beads are marked with biotin.

[0027] The preparation method of the goat monoclonal antibody-coated magnetic beads is:

[0028] A. Take 0.25 mg of goat monoclonal antibody and use 5L of 0.2mmol / L PBS (PH7.4) buffer for dialysis at 2~8℃ overnight.

[0029] B. Take out the dialyzed goat monoclonal antibody in an appropriate container, accurately measure the volume and record, and dilute the goat monoclonal antibody to 0.5 mg / mL for use.

[0030] C. Weigh 1.0 mg of BNHS, fully dissolve it with 217 μL of DMSO, and prepare a 10 mmol / L BNHS solution.

[0031] D. Add the prepared BNHS to goat monoclonal antibody at a ratio of 20:1 to the dialysis goat monoclonal antibody, mix well and react at room temperature for 30 minutes. That is, 1.0 mg of antibody was added to 13.3 μL of 10...

Embodiment 2

[0037] A kit for the determination of triiodothyronine, comprising goat monoclonal antibody-coated magnetic beads, ANS dissociation agent and enzyme working solution, and the goat monoclonal antibody-coated magnetic beads are marked with biotin.

[0038] The preparation method of the goat monoclonal antibody-coated magnetic beads is:

[0039] A. Take 0.2 mg of goat monoclonal antibody and use 4.5L of 0.12mol / L PBS (PH7.4) buffer solution for dialysis at 2~8℃ overnight.

[0040] B. Take out the dialyzed goat monoclonal antibody in an appropriate container, accurately measure the volume and record it, and dilute the goat monoclonal antibody to 0.2 mg / mL for use.

[0041] C. Dissolve BNHS in DMSO and prepare an 8mmol / L BNHS solution.

[0042] D. Add the prepared BNHS to goat monoclonal antibody with a ratio of 18:1 to the dialysis goat monoclonal antibody, mix well and react at room temperature for 28 minutes.

[0043] E. Take 1mol / L Tris buffer to stop the reaction, and add 1mol / LTris buff...

Embodiment 3

[0048] A kit for the determination of triiodothyronine, comprising goat monoclonal antibody-coated magnetic beads, ANS dissociation agent and enzyme working solution, and the goat monoclonal antibody-coated magnetic beads are marked with biotin.

[0049] The preparation method of the goat monoclonal antibody-coated magnetic beads is:

[0050] A. Take 0.3 mg of goat monoclonal antibody and use 6L of 0.12mol / L PBS (PH7.4) buffer for dialysis at 2~8℃ overnight.

[0051] B. Take out the dialyzed goat monoclonal antibody in an appropriate container, accurately measure the volume and record, and dilute the goat monoclonal antibody to 0.1 mg / mL for use.

[0052] C. Dissolve BNHS in DMSO to prepare a 11mmol / L BNHS solution.

[0053] D. Add the prepared BNHS to goat monoclonal antibody at a ratio of 21:1 to the dialysis goat monoclonal antibody, mix well and react at room temperature for 31 minutes.

[0054] E. Take 1.1 mol / L Tris buffer to stop the reaction, and add 1.1 mol / LTris buffer to the r...

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Abstract

The invention provides a kit used for determination of triiodothyronine. The invention aims to overcome the problems of poor specificity and poor sensitivity of conventional T3 immunoassay reagents. The kit used for determination of triiodothyronine comprises magnetic beads coated by a goat monoclonal antibody, an ANS dissociation agent and an enzyme operation fluid, wherein the magnetic beads coated by the goat monoclonal antibody is labeled with biotin. The kit provided by the invention employs the magnetic beads coated by the goat monoclonal antibody; magnetic particles (the magnetic beads) are coated by anti-biotin antibody which is the goat monoclonal antibody; so the kit has higher specificity and sensitivity compared with the conventional T3 immunoassay reagents.

Description

Technical field [0001] The invention relates to a kit, in particular to a kit for determining triiodothyronine. Background technique [0002] Triiodothyronine (T3) is a small molecule project. The antibodies of existing T3 immunoassay reagents are generally polyclonal antibodies or mouse monoclonal antibodies, which are lacking in specificity and sensitivity. The prior art does not dilute the antibody to be labeled to a fixed concentration, and it is inconvenient to operate when labeling a small amount of antibody. At the same time, the difference between batches is large, and the function difference of kits of different batches is large, resulting in low stability. Summary of the invention [0003] The invention provides a kit for determining triiodothyronine, which aims to solve the problem of poor specificity and sensitivity of T3 immunoassay reagents in the prior art. [0004] In order to solve the above technical problems, the present invention is achieved through the followin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54326G01N33/577
Inventor 李玲
Owner SICHUAN ORIENTER BIOLOGICAL TECH
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