Deer collagen peptide lipidosome having blood glucose reducing function, and preparation and application thereof
A liposome and blood sugar-lowering technology, which is applied in the direction of liposome delivery, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problem of losing biological activity, etc., and achieve simple methods, easy large-scale production, The effect of expanding the range of application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] Prepare deer collagen peptide liposomes according to the following process:
[0022] Using fresh sika deer skin as the raw material, the deer skin is boiled at 50℃ for 30 minutes with a mass concentration of 10% NaOH for degreasing treatment and then cleaned with pure water, cut into pieces of 2 to 3 square centimeters, and the deer skin pieces are in accordance with 1:80 ( Material: buffer mass ratio) was added 0.01M acetate buffer, then pepsin was added at a mass ratio of 1:10 (enzyme: raw material), and the reaction was stirred at 40°C for 1 hour. Adjust the pH of the above reaction solution to 7 with 1M NaOH, add complex enzymes (trypsin, alkaline protease, mass mixing ratio 1:1) for enzymatic hydrolysis, the amount of enzyme added is 0.1% of the mass of the reactant raw materials, at 20°C The reaction was stirred for 1 hour, and the reaction was inactivated at 90°C for 20 minutes. Centrifuge the above reaction solution at 5000 rpm for 5 min, collect the supernatant, ...
Embodiment 2
[0025] Using fresh sika deer tendon as raw material, the deer tendon is boiled in 5% NaOH at 80°C for 10 minutes for degreasing treatment and then cleaned with pure water, cut into pieces of 2 to 3 square centimeters. The deer tendon pieces are according to 1:100 (raw material : Buffer mass ratio) add 0.01M phosphate buffer, then add trypsin at a mass ratio of 1:500 (enzyme: raw material), and react with stirring at 35°C for 10 hours. Adjust the pH of the above reaction solution to 6 with 0.1M HCI, add complex enzymes (papain, bromelain, mass ratio 1:1) for enzymatic hydrolysis, the amount of enzyme added is 1% of the reactant mass, and the reaction is stirred at 50°C After 10 hours, the reaction was inactivated at 100°C for 5 minutes. Centrifuge the above reaction solution at 2000 rpm for 10 min, collect the supernatant, and freeze-dry to obtain deer collagen peptide.
[0026] Take 50 mg of a mixture of dry phospholipids and cholesterol, the mass ratio of phospholipids to chole...
Embodiment 3
[0028] Using fresh red deer tendon as raw material, deer tendon is boiled with 30% NaOH by mass concentration at 70°C for 10 minutes for degreasing treatment and then cleaned with pure water. After ethanol degreasing treatment, it is cleaned with pure water and cut into pieces of 2 to 3 square centimeters. The deer tendon fragments were added with 0.01M phosphate buffer at a mass ratio of 1:10 (raw material: buffer), and then neutral protease was added at a weight ratio of 1:50 (enzyme: raw material), and the reaction was stirred at 45°C for 5 hours. Adjust the pH of the above reaction solution to 8 with 0.5M NaOH, add complex enzymes (papain, alkaline protease, flavor protease, mass ratio 1:1:1) for enzymatic hydrolysis, the amount of enzyme added is 0.5% of the mass of the reactant , Stir the reaction at 60°C for 5 hours, and inactivate at 70°C for 20 minutes after the reaction. Centrifuge the above reaction solution at 8000 rpm for 5 min, collect the supernatant, and freeze-...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More