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Blood miR-3135b detection kit used for diagnosis of coronary artery vascular calcification

A technology for blood detection and vascular calcification, applied in the field of blood detection kits and diagnostic blood detection kits

Pending Publication Date: 2017-04-05
SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Many microRNA molecules related to cardiovascular system diseases have been found, which are involved in the regulation of myocardial hypertrophy, vascular endothelial cell function and activity, endothelial cell and smooth muscle cell function, but there is no microRNA in serum / plasma as a role for coronary artery calcification. Reporting of markers

Method used

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  • Blood miR-3135b detection kit used for diagnosis of coronary artery vascular calcification
  • Blood miR-3135b detection kit used for diagnosis of coronary artery vascular calcification
  • Blood miR-3135b detection kit used for diagnosis of coronary artery vascular calcification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A significant positive correlation was confirmed between the expression of miRNA-3135b in serum and the integral level of coronary artery calcium detected in patients.

[0045] Preparation of plasma samples

[0046] 1). Use EDTA anticoagulant tubes to collect blood from the subject, store it at room temperature or at 4 degrees, and proceed to the next step within one hour.

[0047] 2). Centrifuge at 1900g (3000rpm) at 4 degrees for 10 minutes

[0048] 3). Take the upper yellow liquid as plasma

[0049] 4). Centrifuge at 16000g for 10 minutes

[0050] 5). Take the supernatant and store it at -80°C for later use.

[0051] Extraction of miRNA from plasma

[0052] Use miRNeasy Serum / Plasma kit (217184) and miRNeasy Serum / Plasma Spike-in-control (219610) from QIAGEN, USA.

[0053] The specific method is as follows:

[0054] 1). Thaw the serum stored at -80 degrees, and take 200ul each.

[0055] 2). Add 1ml of QIAzol Lysis Reagent (provided with the kit), and mix thoro...

Embodiment 2

[0089] Example 2 Detection rate verification

[0090] Subject recruitment criteria:

[0091] Male aged 50-70 years old, in good health, 162-175cm in height, no drug dependence, no mental, psychological disease, genetic disease and infectious disease history; no history of severe allergies; no malnutrition.

[0092] Clinical detection indicators:

[0093] (1) Coronary CT angiography, calculate coronary artery calcification score

[0094] (2) Detection of expression level of miRNA-3135b in serum

[0095] The extraction, detection and result analysis of miR-3135b in the subject's serum are the same as in Example 1.

[0096] Experimental results:

[0097] According to the current clinical coronary artery calcification detection standard, through coronary CT detection, a CAS greater than 100 is a patient with coronary artery calcification. In this study, the serum of 200 subjects was collected and tested according to the above-mentioned Example 1. The microRNA copy number was ...

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Abstract

The invention relates to a blood miR-3135b detection kit used for diagnosis of coronary artery vascular calcification, which includes a primer for amplifying miR-3135b. In addition, the invention also relates to an application of such primer for amplifying the miR-3135b in preparation of the blood detection kit for diagnosis of the coronary artery vascular calcification.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and in particular relates to a blood detection kit which can be used for the diagnosis of coronary artery calcification. In addition, the present invention also relates to the use of primers for amplifying miR-3135b in the preparation of blood detection kits that can be used for the diagnosis of coronary artery calcification. Background technique [0002] Coronary artery calcification was considered to be a benign aging phenomenon that occurs with age when it was first discovered. a very important risk factor. Coronary artery calcification can lead to decreased vascular compliance, abnormal vasoconstriction response, and even hinder myocardial perfusion. Among people over 70 years old, 90% of men and 67% of women have coronary artery calcification. Many factors, such as high blood pressure, diabetes, dyslipidemia, overweight and obesity, smoking, etc., have been proved to be the cause of coro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李英贤刘巍凌树宽李玉恒周玉杰孙维佳钟国徽
Owner SCI RES TRAINING CENT FOR CHINESE ASTRONAUTS
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