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Thin layer identification method of rhamnus frangula bark and rhamnus purshiana bark

An identification method and technology of beautiful mice, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of strong specificity, intuitive test results, and simple operation

Inactive Publication Date: 2017-04-05
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a thin-layer identification method of buckthorn bark and buckthorn bark, which mainly solves the problem that the prior art cannot realize fast and accurate visual identification of buckthorn bark and buckthorn bark

Method used

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  • Thin layer identification method of rhamnus frangula bark and rhamnus purshiana bark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Buckthorn bark and buckthorn bark raw materials are crushed through an 80-mesh sieve, then add 6 times the amount of water to reflux and extract for 30 minutes, take it out, let it cool, filter, add 5ml of 1mol / L hydrochloric acid to the filtrate, add ethyl acetate saturated with water The ester was extracted twice, 20ml each time, the ethyl acetate layers were combined, the solvent was evaporated to dryness, the residue was dissolved in methanol, filtered through a microporous membrane, and the filtrate was used as the sample solution. Spot the sample solution on the silica gel G thin-layer plate respectively, and the distance between the spotting origin and the lower edge of the thin-layer plate is 1.0 cm.

[0032] Use ethyl acetate, methanol and water at a volume ratio of (10:1.3:1.0) to mix 20mL of developer, and place the developer in a 10×10cm double-tank development cylinder. The amount of developer in the two tanks should be the same. Seal the development cylind...

Embodiment 2

[0037] Buckthorn bark and buckthorn bark raw materials are crushed through an 80-mesh sieve, then add 8 times the amount of water to reflux and extract for 30 minutes, take it out, let it cool, filter, add 8ml of 1mol / L hydrochloric acid to the filtrate, add ethyl acetate saturated with water The ester was extracted twice, 20ml each time, the ethyl acetate layers were combined, the solvent was evaporated to dryness, the residue was dissolved in methanol, filtered through a microporous membrane, and the filtrate was used as the sample solution. Spot the sample solution on the silica gel G thin-layer plate respectively, and the distance between the spotting origin and the lower edge of the thin-layer plate is 1.0 cm.

[0038] Use ethyl acetate, methanol and water with a volume ratio of (8.0:1.0:0.8) to mix 20mL of developer, and place the developer in a 10×10cm double-tank development cylinder. The amount of developer in the two tanks should be the same. Seal the development cyl...

Embodiment 3

[0043] Buckthorn bark and buckthorn bark raw materials are crushed through an 80-mesh sieve, then add 10 times the amount of water to reflux and extract for 30 minutes, take it out, let it cool, filter, add 5ml of 1mol / L hydrochloric acid to the filtrate, add ethyl acetate saturated with water The ester was extracted twice, 20ml each time, the ethyl acetate layers were combined, the solvent was evaporated to dryness, the residue was dissolved in methanol, filtered through a microporous membrane, and the filtrate was used as the sample solution. Spot the sample solution on the silica gel G thin-layer plate respectively, and the distance between the spotting origin and the lower edge of the thin-layer plate is 1.5cm.

[0044] Use ethyl acetate, methanol and water at a volume ratio of (5:0.8:0.6) to mix 20mL of developer, and place the developer in a 10×10cm double-tank development cylinder. The amount of developer in the two tanks should be the same. Seal the development cylinde...

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Abstract

The invention provides a thin layer identification method of rhamnus frangula bark and rhamnus purshiana bark. The method includes the steps of: 1) medicinal material treatment; 2) thin layer sample application; 3) developing solvent preparation; 4) development; 5) color developing and viewing; and 6) result determination. On a thin-layer plate, rhamnus purshiana bark shows yellow and blue spots, rhamnus frangula bark shows yellow, blue and red spots. The method provided by the invention has the advantages of strong specificity, simple operation, visual detection result, and easy determination, can quickly and accurately perform visual identification of hamnus frangula and rhamnus purshiana medicinal materials, and provides a reliable detection method for ensuring the correctness of medicinal material variety during medication.

Description

technical field [0001] The invention belongs to the technical field of identification of medicinal materials, and relates to a method for thin-layer identification of buckthorn bark and buckthorn bark. Background technique [0002] Buckthorn bark, also known as Frangula bark, is the bark of Rhamnus Frangula L., a plant of the genus Rhamnus in the family Rhamnaceae. It is native to Europe and the Soviet Union, and is also distributed in western Asia and northern Africa. Buckthorn bark mainly contains frangunine, frangulanime, frangulin A, frangulin B and glucofrangulin and other chemical components. Buckthorn bark is used medicinally. In addition to treating abdominal pain, vomiting and diarrhea, and treating frequent constipation, it also clears away heat and dampness, eliminates accumulation and kills insects, and treats edema, abdominal distension, hernia, scrofula, scabies, and toothache. pain effect. [0003] American buckthorn bark, also known as Bosch buckthorn bar...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 高娟左志超惠玉虎王春德
Owner SHAANXI JIAHE PHYTOCHEM
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