A method for preparing machine-transplanted rice seedling-raising substrate by using fermented rice straw as raw material
A technology for raising rice seedlings and fermented products, which is applied in the field of machine-transplanted rice seedling raising substrates and its preparation, which can solve the problems of large amount of nutrient soil, large operating machinery load, difficult degradation of substrates, and high cost, and achieves reduced irrigation times and low-cost methods , the effect of avoiding pollution
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Embodiment 1
[0030] Example 1: Preparation of rice seedling-raising substrate by single bacterial agent fermentation + biocontrol bacteria
[0031] Such as figure 1 As shown, the mixture of rice stalks and fresh pig manure was fermented with beneficial bacteria to prepare organic fertilizer. The organic fertilizer was used as the main material, and auxiliary materials and activated solid biocontrol agents were added to prepare rice seedling raising substrate. The specific steps were as follows:
[0032] (1) Preparation of bacterial agent: Trichoderma viride, Aspergillus niger and Bacillus subtilis are beneficial bacteria for aerobic fermentation. Trichoderma viride and Aspergillus niger were cultured in potato glucose liquid medium, 180 rpm, 28°C for 5 days; Bacillus subtilis was cultured in beef extract peptone liquid medium, 180 rpm, 37°C for 2 days;
[0033](2) Rice stalk fermentation: static aerobic high-temperature composting is carried out on the cement floor of the greenhouse, and ...
Embodiment 2
[0051] Example 2: Two-component compound bacterial agent + biocontrol bacteria prepare rice seedling-raising matrix
[0052] In the following steps, unless otherwise specified, the same method as in Example 1 was used for processing.
[0053] (1) Preparation of bacterial agents: Saccharomyces cerevisiae, Trichoderma viride, and Aspergillus niger were cultivated with potato dextrose liquid medium at 180 rpm and 28°C for 5 days; Bacillus subtilis was cultivated with beef extract peptone liquid medium at 180 rpm / min and cultured at 37°C for 2 days.
[0054] (2) Rice stalk fermentation: three inoculum treatment groups, namely Bacillus subtilis + Saccharomyces cerevisiae group, Trichoderma viride + Aspergillus niger group, Aspergillus niger + Saccharomyces cerevisiae group, the inoculum inoculation amount was 0.1% of the stacking mass %, 0.4%, 1.0%, ferment according to the method described in Example 1, when the temperature of rice straw and pig manure fermented product drops to...
Embodiment 3
[0071] Example 3: Fermenting rice stalks with six-component compound bacterial agents to prepare rice seedling-raising substrates
[0072] (1) Preparation of inoculum: two inoculum treatment groups are set up altogether, one group is the treatment group not inoculated with inoculum, and the other is the treatment group inoculated with liquid fermentation inoculum; the preparation and inoculation methods of liquid fermentation inoculum are : Saccharomyces cerevisiae, Trichoderma viride, and Aspergillus niger were cultured in potato glucose liquid medium at 180 rpm and 28°C for 5 days, and Acinetobacter, Bacillus subtilis, and Bacillus thuringiensis were cultured in beef extract peptone liquid medium at 180 rpm / min and cultured at 37°C for 2 days. Centrifuge the six kinds of bacterial solutions at 5000 rpm, remove the supernatant, wash the bacterial cells three times with a phosphate buffer solution with a pH of 7, and then suspend the bacterial cells with 1 liter of phosphate ...
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