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Fluorescent LAMP primer for detecting infectious hematopoietic necrosis virus of fish

A hematopoietic organ necrosis and infectivity technology, which is applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc. Improved sensitivity and good repeatability

Inactive Publication Date: 2017-04-26
珠海出入境检验检疫局检验检疫技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a fluorescent LAMP primer for detecting fish infectious hematopoietic organ necrosis virus, aiming to solve the problems of long detection period, cumbersome steps, large workload and high cost in the current monitoring method of fish infectious hematopoietic organ necrosis virus. high problem

Method used

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  • Fluorescent LAMP primer for detecting infectious hematopoietic necrosis virus of fish
  • Fluorescent LAMP primer for detecting infectious hematopoietic necrosis virus of fish
  • Fluorescent LAMP primer for detecting infectious hematopoietic necrosis virus of fish

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0025] The sequence of the fluorescent LAMP primer used to detect fish infectious hematopoietic necrosis virus provided by the embodiment of the present invention is:

[0026] SEQ ID NO: 1

[0027] FIP GCCTCTGCTACGATCCTCATCGCATGAAGTCAGTGGTGG

[0028] SEQ ID NO: 2

[0029] BIP GTACGGTGTCATGATGAGCAGGCTGGCGTTCTCGTTGATC

[0030] SEQ ID NO: 3

[0031] F3GGACTGTTCAACCAAGCC

[0032] SEQ ID NO: 4

[0033] B3ATCGTCATACCTATCGTTGATG

[0034] SEQ ID NO: 5

[0035] LF AGTCTGACAATGCGTCTGG ...

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Abstract

The invention discloses a fluorescent LAMP primer for detecting infectious hematopoietic necrosis virus of a fish, sequences of the fluorescent LAMP primer for detecting the infectious hematopoietic necrosis virus of the fish are shown as follows: SEQIDNO:1, SEQIDNO:2, SEQIDNO:3, SEQIDNO:4, SEQIDNO:5 and SEQIDNO:6. A fluorescent LAMP detection technology is established by introduction of a fluorescent dye based on an original LAMP technology, due to the use of instruments for fluorescence collection, the detection sensitivity is further improved, the fluorescent LAMP detection technology is more objective and more convenient to operate, and the fluorescent LAMP detection technology is easy to standardize and easy to popularize and use.

Description

technical field [0001] The invention belongs to the technical field of detection of fish infectious hematopoietic organ necrosis virus, in particular to a fluorescent LAMP primer for detecting fish infectious hematopoietic organ necrosis virus. Background technique [0002] Infectious hematopoietic necrosis virus (IHNV) is an important fish rhabdovirus affecting aquaculture. IHNV belongs to the genus of extragranular rhabdovirus (Novirhabddovirus), is the pathogen that causes infectious hematopoietic organ necrosis, and mainly infects salmon and trout. The virus is widely distributed in many parts of Europe, America and Asia. It is an acute, hemorrhagic and highly fatal viral disease. Especially for fry and juvenile fish, it can cause a higher mortality rate, which can reach more than 90% in severe cases. Once the fish infectious hematopoietic necrosis (IHN) caused by the virus breaks out, it will bring catastrophic losses to the aquaculture industry, and has been listed a...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2563/107
Inventor 薄清如罗宝正徐海聂赵福振王小玉邵建宏廖秀云林志达
Owner 珠海出入境检验检疫局检验检疫技术中心
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