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Method used for increasing industrial avermectin B<1a> yield via optimization of fermentation medium

A technology of fermentation medium and avermectin, which is applied in the field of fermentation biology and can solve the problem of low B1a production

Active Publication Date: 2017-05-03
上海国佳生化工程技术研究中心有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the biofermentation process using Streptomyces avermitilis to produce abamectin on a large scale in industry, there has always been a B 1a The phenomenon of low yield

Method used

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  • Method used for increasing industrial avermectin B&lt;1a&gt; yield via optimization of fermentation medium
  • Method used for increasing industrial avermectin B&lt;1a&gt; yield via optimization of fermentation medium
  • Method used for increasing industrial avermectin B&lt;1a&gt; yield via optimization of fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1, fermentation shake flask culture

[0086] Three groups of different calcium salts with different concentrations of 0.1g / L, 0.2g / L, and 0.3g / L were added to the fermentation medium, as shown in Table 4.

[0087] Table 4. Calcium salt addition scheme

[0088]

[0089] Seed shake bottle: dig 1cm with an inoculation shovel 2 The seed slant was inoculated into the seed shaker flask for cultivation, the size of the seed shaker flask was 500ml, the filling volume was 60ml, the rotating speed of the shaker was 250rpm / min, and the cultivation was carried out at a constant temperature of 28°C for 2 days. The seed shake flask does not add calcium chloride, no induction, no stirring, no aeration.

[0090] Fermentation shake flask: take 2ml of the cultivated seed liquid from the seed shake flask and put it into the fermentation shake flask for fermentation and cultivation. The fermentation shake flask is 500ml, the liquid volume is 40ml, the shaker speed is 250rpm...

Embodiment 2

[0094] Embodiment 2, fermentor cultivation

[0095] The 100L advanced fermenter is used as a seed tank, and the 500L advanced fermenter is used as a fermenter. The fermentation medium of the control group was not changed, and 0.1 g / L of calcium chloride was added to the fermentation medium of the experimental group.

[0096] seed jar

[0097] A 100L high-grade fermenter is used. After digestion, the pH was adjusted to 7.5, the volume of the fermentation broth was 60L, the culture temperature was 28°C, the tank pressure was 0.05MPa, the ventilation volume was 1:1.5 (vvm), and the fermentation was started for 25 hours with stirring at 300rpm. The method is pipe inoculation, and the amount of seed conversion is 8%. There were no other manipulated variables during the culture, ie no calcium chloride addition, no induction.

[0098] Fermenter

[0099] A 500L high-grade fermenter is used. After digestion, the pH was adjusted to 7.2, the volume of the fermentation broth was 300...

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Abstract

The invention relates to a method used for increasing industrial avermectin B<1a> yield via optimization of fermentation medium. It is found via large scale of screening that adding of an appropriate concentration of calcium chloride into streptomyces avermitilis fermentation medium is capable of increasing B<1a> yield obviously. The invention also provides a medium used for the method.

Description

technical field [0001] The invention belongs to the field of fermentation biology, more specifically, the invention relates to a method of improving industrial abamectin B by optimizing the fermentation medium 1a yield method. Background technique [0002] Abamectins are a group of sixteen-membered macrolide antibiotics produced by Streptomyces aver-mitilis (Burg R, Miller B, Baker E. Avermectins, new family ofpotent anthelmintic agents: producing organism and fermentation.Antimicrobial agents and chemotherapy.1979, 15(3):361), has a wide range of biological activities such as insecticidal and antiparasitic, and plays an important role in medicine, agriculture and animal husbandry (Campbell W C, Burg R, Fisher M .The discovery of ivermectin and other avermectins. 1984, PesticideSynthesis Through Rational Approaches.Chapter 1:5-20). [0003] Abamectin contains eight components A 1a , A 1b , A 2a , A 2b , B 1a , B 1b , B 2a , B 2b , but only B 1a and B 1b Has medic...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/465
Inventor 陈虎杨黎亮郭美锦储炬庄英萍张嗣良王太富樊乐王得明
Owner 上海国佳生化工程技术研究中心有限公司
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