Mallotus oblongifolius callus culture method and culture medium thereof

A medium and benchmark technology, applied in the agricultural field, can solve the problems of inability to obtain callus materials, shortage, easy browning and the like

Inactive Publication Date: 2017-05-10
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural pollination conditions, the average seed setting rate (number of mature seeds / female flowers) of partridge tea is less than 5%. Therefore, the industry has turned its attention to tissue culture, but the tissue culture of partridge tea has always been a bottleneck in the industry, because explanted Somatic culture is prone to browning, and callus material cannot be obtained

Method used

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  • Mallotus oblongifolius callus culture method and culture medium thereof
  • Mallotus oblongifolius callus culture method and culture medium thereof
  • Mallotus oblongifolius callus culture method and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-9

[0021] Embodiment 1-9 (medium)

[0022] Embodiment 1-9 all adopts MS as basic medium, pH value is 5.8-6.2, wherein adds 30g / L sucrose and 7.5g / L agar, and adds hormone NAA (naphthalene acetic acid) and 2,4-D, each The amounts of NAA and 2,4-D added in the examples are shown in Table 1.

Embodiment 10-18

[0023] Embodiment 10-18 (medium)

[0024] Embodiment 10-18 all adopts MS as basic medium, and pH value is 5.8-6.2, wherein adds 30g / L sucrose and 7.5g / L agar, and adds hormone NAA (naphthalene acetic acid) and KT (kinetin), each The amount of NAA and KT added in the examples is shown in Table 2.

Embodiment 19-27

[0025] Embodiment 19-27 (medium)

[0026] Embodiment 19-27 all adopts MS as basic medium, and pH value is 5.8-6.2, wherein adds 30g / L sucrose and 7.5g / L agar, and adds hormone NAA (naphthalene acetic acid) and 6-BA (6-benzylamino adenine), NAA and 6-BA additions are as shown in table 3 in each embodiment.

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Abstract

The invention provides a culture medium for culturing mallotus oblongifolius callus. The culture medium is prepared from two hormone components, wherein the first hormone component is naphthylacetic acid, the content of the first hormone component is 0.1 to 0.5mg / L taking the culture medium as the standard, the second hormone component is prepared from kinetin and 2,4-D or 6-benzylaminopurine, and the content of the second hormone component is 0.5 to 1.5mg / L taking the culture medium as the standard. The invention further provides a mallotus oblongifolius callus culture method which comprises the steps of taking robust and sterile mallotus oblongifolius seedling hypocotyl, cutting into small sections and inoculating to the culture medium to perform induction culture. The culture medium which can effectively culture the mallotus oblongifolius callus is obtained through repeated explorations by the inventor of the application in order to overcome the defects in the prior art; when the culture medium is used for culturing the mallotus oblongifolius callus, the callus rate is high; thus, the blank of mallotus oblongifolius tissue culture is filled.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for cultivating partridge tea callus and a culture medium thereof. Background technique [0002] Partridge tea [Mallotus peltatus (Geiseler) Muller Argoviensis] is also called Shankucha, Maocha and Hegucha, and it is a plant of the genus Euphorbiaceae. Partridge tea is mainly distributed in Wenchang, Wanning and Lingshui in Hainan Island, and also in Sanya, Ledong, Baisha, Ding'an, Dongfang, Qiongzhong, Baoting and Changjiang. Among them, Wenchang Tongguling and Wanning The partridge tea produced in Dongshanling is the most famous. In addition, there are also wild distributions in Guangdong, Yunnan, Guangxi, China-India Peninsula and Sumatra Island. Partridge tea is round and sweet. It is a peculiar wild tea. Its leaves and other plants have a strong Lingling aroma after drying; when brewed and drunk, it has a unique flavor, sweet and refreshing. ". Partridge t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00
Inventor 杨虎彪刘国道李晓霞白昌军王文强
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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