Saccharomyces cerevisiae expressed long-acting recombinant fibronectin and application thereof in cosmetics

A technology for fibronectin and Saccharomyces cerevisiae, applied in the emerging biomedical field, can solve the problems of low feasibility of full sequence expression, large molecular weight of natural FN, and low biological activity of FN protein.
CN113186109AInactive Publication Date: 2021-07-30WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
Publication Date
2021-07-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a saccharomyces cerevisiae expressed long-acting recombinant fibronectin and an application of the long-acting recombinant fibronectin in cosmetics. The nucleotide sequence of the long-acting recombinant fibronectin is shown as Seq ID NO.1, and the long-acting recombinant fibronectin comprises a functional fragment of FN and HAS; the functional fragment of the FN comprises an FNIII1C unit; and the FNIII1C unit is a functional fragment at the C end of the first III-type FN repetitive unit in the FN. FNIII1C is a functional fragment at the C end of a first III type FN repetitive unit in natural human FN, and has the functions of resisting tumors, resisting metastasis and inhibiting angiogenesis. The protein can also enable FN in a living body to be subjected to super-polymerization, and the cell adhesion promoting and cell migration resisting capabilities of the FN are greatly enhanced. The fusion HAS effectively improves the stability of the recombinant protein. The long-acting recombinant fibronectin disclosed by the invention can be used for improving the adherence rate and convergence rate of cells, shortening the cell convergence time, enabling the morphological structure of the cells to be good, enhancing the metabolic rate and remarkably improving the protein synthesis speed, and has the effect of promoting cell repair.
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Description

technical field

[0001] The present invention relates to the emerging field of biomedicine, in particular to a long-acting recombinant fibronectin expressed by Saccharomyces cerevisiae, a detection method for detecting the adhesion-promoting activity of bovine kidney cells by long-acting recombinant fibronectin, and a long-acting recombinant fibronectin expressed by Saccharomyces cerevisiae Application of recombinant fibronectin in cosmetics. Background technique

[0002] Natural fibronectin (fibronectin, FN) is a high molecular weight glycoprotein and one of the most important components of the extracellular matrix, widely present in blood, body fluids and various tissues. In vertebrates, FN is highly conserved and has the same organizational structure. FN exists in the form of a dimer, which is composed of two polypeptide chains with a molecular weight of about 250 kDa connected by a disulfide bond at the C-terminus. Each chain contains a series of repeat units: 12 type I...

Claims

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