Application of selenomethionine
A technology of selenomethionine and health products, applied in the application field of selenomethionine, can solve problems such as the research of selenium compounds, achieve significant curative effect, improve memory and cognition
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[0053] Example 1
[0054] Selenomethionine (Se-Met) was added to the cell culture medium of neural stem cells (NSCs) at a concentration of 10 μM to observe its proliferation effect on neural stem cells. The CCK-8 method was used to detect the effects of different concentrations of Se-Met on the viability of neural stem cells; the proliferation of neural stem cells was detected by 5-bromodeoxyuridine (BrdU) and DAPI immunofluorescence after neural stem cells were pretreated with Se-Met; The effect of Se-Met on neural stem cell proliferation is determined by morphological observation and analysis to detect the rate of neural stem cell aggregation into neurospheres; Western blotting (Westen Blot) is used to detect protein changes related to neural stem cell proliferation to verify the effect of Se-Met on Proliferation of neural stem cells.
[0055] (1) CCK-8 detects the vitality of neural stem cells, the results are: see figure 1 The effect of selenomethionine on the vitality of neur...
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[0063] Example 2
[0064] In the pathogenesis of some neurodegenerative diseases, the loss and apoptosis of neurons are important reasons that affect cognition, and the regeneration of nerves in the lower layer of the dentate gyrus of the hippocampus is inhibited, which in turn leads to obstacles in the differentiation of new neural stem cells into neurons It is also considered to be the main cause of cognitive impairment. Therefore, this example explores whether Se-Met can promote the differentiation of NSCs into neurons, and then repair the damaged nervous system.
[0065] After changing the culture medium of neural stem cells, adding Se-Met for 14 days, the adherent neurospheres began to grow nerve fiber filaments around, and the number of nerve fiber filaments growing around the neurospheres in the Se-Met group was significantly more than In the control group, microtubule-associated protein (MAP2)-labeled neurons were found around the neurospheres by immunofluorescence staining...
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[0066] Example 3
[0067] In this example, the effect of Se-Met on neural stem cells and neural stem cell differentiation into neurons was studied at the animal level.
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