Method for Measuring Total Amino Acids in Tobacco Leaf Infested with Pesticides
A measurement method and amino acid technology are applied in the field of measuring the total amount of amino acids in tobacco leaves that are damaged by pesticides, and can solve the problems of inaccurate determination, difficulty in determining the degree of chloroquinolinic acid damage to tobacco, and damage to tobacco leaves, and achieve the effect of accurate measurement.
Inactive Publication Date: 2019-11-01
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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Problems solved by technology
[0006] The invention proposes a method for measuring the total amount of amino acids in tobacco leaves damaged by pesticides, which solves the problem in the prior art that it is difficult to measure the degree of damage to tobacco caused by quinclorac, or the measurement is not accurate enough
Method used
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Embodiment 1
[0030] Tobacco seeds used variety K326. Greenhouse sowing, raising seedlings, transplanting at the stage of five to six true leaves, and measuring the total amount of amino acids in tobacco leaves affected by pesticides
[0031] (1) Reagent preparation
[0032] Citric acid buffer at pH 5.0: Weigh 4.31 g of citric acid and 8.68 g of sodium citrate, dissolve in distilled water and dilute 500 mL.
[0033] 1% hydrated ninhydrin solution: Weigh 1 gram of hydrated ninhydrin and dissolve it in 100 mL of 95% ethanol.
[0034] 0.1% ascorbic acid solution: Weigh 0.05 g of ascorbic acid, dissolve it in distilled water and dilute to 50 mL.
[0035] Standard sodium glutamate solution: Accurately measure 0.1883 grams of edible monosodium glutamate (containing 80% sodium glutamate, while sodium glutamate contains 8.3% nitrogen), dry it at 80°C (about 2 hours), and then dry the dried Edible monosodium glutamate was dissolved in a pH 5.0 citric acid buffer, and the volume was adjusted to 50...
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Abstract
The invention provides a method for measuring the total amount of amino acids in tobacco leaves damaged by pesticides. The method comprises the following steps: (1) preparing a reagent composed of a citric acid buffer solution with a pH value of 5.0, a ninhydrin hydrate solution with a concentration of 1%, an ascorbic acid solution with a concentration of 0.1% and a standard monosodium glutamate solution; (2) drafting a standard work curve; (3) extracting amino acids; (4) measuring the total amount of the amino acids; and (5) transferring tobacco in a five-to-six-true-leaf stage into toxic soil with quinclorac concentrations of 1.04*10<-3> mg / kg, 2.08*10<-3> mg / kg, 4.17*10<-3> mg / kg, 8.33*10<-3> mg / kg and 1.67*10<-3> mg / kg, respectively, and measuring the total amounts of amino acids according to the steps (1) to (4) in 7 d, 14 d, 21 d, 28 d and 35 d respectively. Thus, the measured total amount of the amino acids in tobacco leaves damaged by quinclorac is more accurate and reliable; and scientific bases are provided for rational usage of quinclorac and elimination of the influence of quinclorac.
Description
technical field [0001] The invention relates to the field of chemical analysis and detection, in particular to a method for measuring the total amount of amino acids in tobacco leaves damaged by pesticides. Background technique [0002] Quinolinic acid is a selective herbicide for the control of barnyardgrass in paddy fields. It is a hormone-type quinoline carboxylic acid herbicide. The symptoms of weed poisoning are similar to those of auxins. It is mainly used for the control of barnyardgrass and has a long pot life. 1-7 leaf stage are effective. Rice is safe. [0003] Relevant studies have shown that quinclorac, as a hormonal herbicide, can stimulate the accumulation of ethylene in plants. The target site of quinclorac is located in the biosynthesis of cell wall, and it acts as an inhibitor of cell wall synthesis in Gramineae. The sensitivity of different Gramine action sites to quinclorac. Ethylene can inhibit the activity of cell wall synthase and reduce the synthesi...
Claims
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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL