Chemiluminescent kit for quantitatively detecting mycobacterium tuberculosis r interferon as well as preparation method, detection method and evaluation method of chemiluminescent kit

Abstract

The invention provides a chemiluminescent kit for a quantitatively detecting mycobacterium tuberculosis r interferon as well as a preparation method, detection method and evaluation method of the chemiluminescent kit. The chemiluminescent kit comprises a blood sample stimulation system and an immunity detection system, wherein the blood sample stimulation system comprises a culture tube, a blood sample stimulation reference substance and a tuberculosis specificity stimulation protein; and the blood sample stimulation system comprises an r interferon clone antibody coated chemiluminescent panel, a biotin labeled r interferon clone antibody, a streptavidin labeled enzyme substrate and an enzyme-promoting luminescent substrate. According to the chemiluminescent kit, an enzyme-promoting chemiluminescent method is adopted, an r interferon monoclonal antibody or r interferon polyclonal antibody in the chemiluminescent panel is taken as a capture antibody, and a biotin labeled r interferon monoclonal antibody or biotin labeled r interferon polyclonal antibody is taken as a detection antibody, so that the detection sensitivity and accuracy of the kit are improved, the high-flux sample detection can be conveniently realized, and the consumption of samples is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to a chemiluminescence kit for quantitatively detecting mycobacterium tuberculosis r-interferon and a preparation method, a detection method and an evaluation method thereof. Background technique [0002] Tuberculosis is still one of the major infectious diseases that endanger human health and life all over the world. At present, there are about 20 million tuberculosis patients in the world, about 8 million new tuberculosis patients are added every year, and about 3 million people die from tuberculosis every year. my country ranks second in the world after India. According to the preliminary results of epidemiological sampling surveys in recent years, about 500 million people in my country are infected with Mycobacterium tuberculosis, there are about 5 million tuberculosis patients, and about 100,000 people die from tuberculosis every year, ranking first among infectious di...

AI Technical Summary

Problems solved by technology

The detection sensitivity of ELISPOT detection is high, but because this detection method needs to separate and count lymphocytes after taking the patient's blood sample, and then carry out the later stimulation culture overnight and immune detection procedures, the operation is complicated and the professional threshold is high, which greatly limits the detection. In addition, because it involves more supporting equipment and reagents, it also greatly increases the detection cost and the error rate in the operation process.

The use of whole blood culture in ELISA test saves a lot of operation time, and also makes up for the defects of ELISPOT in terms of detection throughput, but due to the limitation of detection methodology, its detection sensitivity is low, and it is suitable for low immunity or lymphocyte count deviation. For people with low blood pressure, the uncertainty of the test results is high, and accurate diagnosis cannot be achieved, and because of the low sensitivity of the test, the amount of blood sample required for testing is also high, requiring no less than 1ml of blood per culture tube quantity

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