Method for detecting proteins based on combination of colorimetric array sensor and mobile phone

An array sensor and protein technology, applied in the direction of color/spectral characteristic measurement, instruments, measuring devices, etc., can solve the problems of high cost, complicated operation, unfavorable site detection, etc., and achieve low cost, simple operation, and simplified signal processing Effect

Inactive Publication Date: 2017-05-24
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology

[0004] Most of the nucleic acid aptamers that have been reported so far are complexed with gold nanoparticles (AuNPs) to form a DNA-AuNPs complex, and the colorimetric array sensor based on this is used for protein recognition (Anal.Chem.2013, 85.6571-6574 ; Anal.Chem.2015, 87.3354-3359), high cost, complex operation, unfavorable for site detection

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  • Method for detecting proteins based on combination of colorimetric array sensor and mobile phone
  • Method for detecting proteins based on combination of colorimetric array sensor and mobile phone
  • Method for detecting proteins based on combination of colorimetric array sensor and mobile phone

Examples

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Embodiment 1

[0036] Example 1 The colorimetric array sensor used in connection with mobile phones is used for the detection of multiple proteins

[0037] Gold nanoparticles (AuNPs) are prepared by sodium citrate reduction of chloroauric acid. As nanometer materials with a particle size range of nanometer level, their synthesis is very simple. The classical method of AuNPs synthesized by citrate reduction of chloroauric acid is the most Convenient.

[0038] The method for detecting protein based on the combination of a colorimetric array sensor and a mobile phone provided in this embodiment includes the following steps:

[0039] 1. Preparation of gold nanoparticles: prepared by reducing chloroauric acid with sodium citrate, the molar ratio of chloroauric acid and sodium citrate is 3.5:1, and the average particle size of the gold nanoparticles in the resulting solution is 13nm.

[0040] 2. Preparation of protein-nano-gold complexes: add 50 μL of 0.5 μM 12 kinds of proteins to 100 μL of the above-men...

Embodiment 2

[0051] Example 2 Practical application of protein detection

[0052] In order to prove the feasibility of the array sensor in Example 1, in the urine of healthy humans diluted 1 times, 12 kinds of proteins at 200nM were detected. Since human urine contains about 155mM NaCl, the urine was diluted 6 Times, 3 times, 2 times and 1.5 times to achieve the NaCl concentration required by the sensor unit. To 200μL of AuNPs were added 100μL of healthy human urine diluted 6 times, 3 times, 2 times, and 1.5 times containing 1μM protein to be tested, and then added 200μL ultrapure water, incubated for 30min, UV detection, the results showed that it can be completely distinguish( Figure 5 ). Using the array sensor in the actual sample, the color difference map corresponding to 200nM multiple proteins is shown in the mobile phone photograph Image 6 .

[0053] Subsequently, the quantification of HSA and Lys and the detection of their mixture were carried out respectively. The photos taken by ...

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Abstract

The invention provides a method for detecting proteins based on combination of a colorimetric array sensor and a mobile phone. Salts of different concentrations and nano-gold are taken as a sensing unit to greatly lower the cost, the method further has the advantages of simple operation, high response speed, high sensitivity and the like, and the aggregation degrees of gold nanoparticles are different under induction of the salts of different concentrations and the proteins to obtain different response optical signals, so that 12 proteins can be distinguished in distilled water, and furthermore, 12 proteins of 200 nM can be completely identified in a urine sample of a healthy person of which the concentration is 50%. Response signals of a sensing system are processed by using a smart phone, thereby being beneficial for simplifying the processing of the signals and shortening the analysis time, and multiple proteins, Lys and HSA of different concentrations and mixtures of Lys and HAS can be identified in the urine sample, so that practical application values of the colorimetric array sensor for protein detection are greatly promoted and the colorimetric array sensor has broad application prospects in the fields of resource shortage, site detection and the like.

Description

Technical field [0001] The invention relates to the technical field of biological macromolecule detection, in particular to a method for detecting proteins based on the combination of a colorimetric array sensor and a mobile phone. Background technique [0002] The detection of biological macromolecules plays an important role in the food industry, biology, biomedicine and other fields. At present, the commonly used analysis methods include enzyme-linked immunoassay, chromatography-mass spectrometry, and gel electrophoresis. Although these technologies have high sensitivity and high specificity, they have disadvantages such as complicated operation, long analysis time, high cost, low efficiency, etc., especially in the practical application such as lack of resources or site inspection. [0003] The use of array sensors for biological macromolecules is a new type of analysis method that has developed rapidly in recent years. This method simulates the mammalian smell or taste system...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/314G01N2021/3155
Inventor 刘月英王飞扬陆跃翔杨家诚景文杰贺刘莹陈莹
Owner CAPITAL NORMAL UNIVERSITY
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