Skin micro-ecological improvement preparation and preparation method thereof
A micro-ecology and skin technology, applied in the field of skin care products, can solve the problems of unsatisfactory active probiotic skin care effects, short shelf life, increased safety hazards, etc., to ensure activity and stability, high stability and activity, overcome palliative effect
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[0034] The preparation of the skin microecology improving preparation adopts the following method to obtain:
[0035] 1. Obtaining culture medium
[0036] 1) Activated bacteria
[0037] The active probiotics are one or more of Lactobacillus acidophilus, Lactobacillus casei subspecies, Bifidobacterium lactis and Streptococcus thermophilus.
[0038] Freeze-dried strains of lactic acid bacteria were taken for activation. The freeze-drying tube was sterilized and cut open, a small amount of medium was added, the bacterial solution was sucked out, plated, and cultured under anaerobic conditions at 37°C for 48 hours. Select colonies that conform to the shape of lactic acid bacteria and have good growth and development, and activate the liquid seed medium. The activation conditions are cultivated under anaerobic conditions at 37°C for 12 to 24 hours to become first-class seeds. The primary seeds are inoculated into the seed medium according to the inoculum amount of 2%-8% (v / v), a...
Embodiment 1
[0061] 1. Activate Lactobacillus casei subspecies cheese in MRS medium for two generations, grow to the logarithmic phase of the bacteria, in the exopolysaccharide selective ESM, 37 ° C for 20 hours of high-density culture, and then use the bench-top fermentation broth Centrifuge at 10,000 rpm for 5 minutes to obtain supernatant.
[0062] 2. The obtained supernatant was sterilized at 90°C for 5 minutes and then cooled to room temperature, and its acidity was measured. The measured pH was 3.72, and the titrated acidity was 159.54°T. The mixed supernatant was divided into two sterilized blue cap bottles middle.
[0063] 3. According to the acidity of the centrifuged supernatant after sterilization, calculate the theoretically neutralized NaHCO per gram of the centrifuged supernatant 3 The amount is 1.34×10 -2 g. According to the establishment of the buffer formula, 1kg of fermentation supernatant neutralizes the NaHCO required to reach pH5.6 3 Added amount 8g NaHCO 3 .
[...
Embodiment 2
[0068] 1. Activate Lactobacillus acidophilus, Lactobacillus casei subsp. casei, and Lactobacillus thermophile in MRS medium for two generations respectively, and the bacteria that have grown to the logarithmic phase are subjected to exopolysaccharide selective ESM at 37°C. After 17 hours of high-density culture, the number of live bacteria in the culture solution reached 1.4×10 9 , 5.5×10 9 , 6×10 9 CFU / g, and then the fermentation broth was centrifuged at 12,000 rpm for 5 min with a desktop centrifuge, and the supernatant was taken.
[0069]2. Mix the obtained supernatant according to the volume ratio of 3:3:4 to obtain the probiotic supernatant. The supernatant was sterilized at 95°C for 5 minutes, then cooled to room temperature, and its acidity was measured. The measured pH was 3.63, and the titrated acidity was 183.80°T.
[0070] 3. According to the acidity of the centrifuged supernatant after sterilization, calculate the theoretically neutralized NaHCO per gram of the...
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