Skin micro-ecological improvement preparation and preparation method thereof

A micro-ecology and skin technology, applied in the field of skin care products, can solve the problems of unsatisfactory active probiotic skin care effects, short shelf life, increased safety hazards, etc., to ensure activity and stability, high stability and activity, overcome palliative effect

Active Publication Date: 2017-05-31
深圳安德瑞医药科技有限公司
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Problems solved by technology

But there are the following disadvantages: 1. Yeast is not a resident bacterium on the skin and cannot colonize the skin, so it cannot participate in the metabolism of the skin, and only a continuous supply can work on the skin, which needs further improvement
2. Candida lambicus and Saccharomyces rubica used are dead bacteria, which cannot further grow and reproduce
3. Grams of Candida and Crimson Saccharomyces do not reach the level of oral probiotics, so applying directly to the facial skin may disrupt the physiological cycle of the human body
[0005] 2. Chinese patent: a cosmetic containing a microecological regulator (CN102755281A), in which the cosmetic contains 0.5% to 1% of bifidobacteria by weight, using bifidobacteria as the microecological regulator of the cosmetic, and producing The micro-ecological skin care products that come out can promote skin metabolism and achieve the effect of skin care and beauty; but there are the following disadvantages: 1. The bifidobacterium used is a strain of Bifidobacterium adolescent, and its physiological metabolic ability is relatively simple
2. Bifidobacterium adolescentis are mainly intestinal probiotics, which are difficult to colonize on the skin
3. The bifidobacterium adolescents used are mainly dead bacteria, which cannot further grow and reproduce, and participate in the physiological cycle of the human body
[0006] 3. Chinese patent: cosmetic method, skin care composition, bacteria and dried bacteria (CN103945829A), in which the invention provides a kind of Staphylococcus epidermidis obtained from the screening of resident microorganisms on the surface of the human body, and the same individual of the sampling object The input and surface proliferation of the obtained bacteria are carried out to improve skin health; however, there are the following disadvantages: 1. The strain is specific, and there is no Staphylococcus epidermidis at the strain level, so it is impossible to determine whether it is a beneficial bacterium, and th

Method used

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  • Skin micro-ecological improvement preparation and preparation method thereof
  • Skin micro-ecological improvement preparation and preparation method thereof

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preparation example Construction

[0034] The preparation of the skin microecology improving preparation adopts the following method to obtain:

[0035] 1. Obtaining culture medium

[0036] 1) Activated bacteria

[0037] The active probiotics are one or more of Lactobacillus acidophilus, Lactobacillus casei subspecies, Bifidobacterium lactis and Streptococcus thermophilus.

[0038] Freeze-dried strains of lactic acid bacteria were taken for activation. The freeze-drying tube was sterilized and cut open, a small amount of medium was added, the bacterial solution was sucked out, plated, and cultured under anaerobic conditions at 37°C for 48 hours. Select colonies that conform to the shape of lactic acid bacteria and have good growth and development, and activate the liquid seed medium. The activation conditions are cultivated under anaerobic conditions at 37°C for 12 to 24 hours to become first-class seeds. The primary seeds are inoculated into the seed medium according to the inoculum amount of 2%-8% (v / v), a...

Embodiment 1

[0061] 1. Activate Lactobacillus casei subspecies cheese in MRS medium for two generations, grow to the logarithmic phase of the bacteria, in the exopolysaccharide selective ESM, 37 ° C for 20 hours of high-density culture, and then use the bench-top fermentation broth Centrifuge at 10,000 rpm for 5 minutes to obtain supernatant.

[0062] 2. The obtained supernatant was sterilized at 90°C for 5 minutes and then cooled to room temperature, and its acidity was measured. The measured pH was 3.72, and the titrated acidity was 159.54°T. The mixed supernatant was divided into two sterilized blue cap bottles middle.

[0063] 3. According to the acidity of the centrifuged supernatant after sterilization, calculate the theoretically neutralized NaHCO per gram of the centrifuged supernatant 3 The amount is 1.34×10 -2 g. According to the establishment of the buffer formula, 1kg of fermentation supernatant neutralizes the NaHCO required to reach pH5.6 3 Added amount 8g NaHCO 3 .

[...

Embodiment 2

[0068] 1. Activate Lactobacillus acidophilus, Lactobacillus casei subsp. casei, and Lactobacillus thermophile in MRS medium for two generations respectively, and the bacteria that have grown to the logarithmic phase are subjected to exopolysaccharide selective ESM at 37°C. After 17 hours of high-density culture, the number of live bacteria in the culture solution reached 1.4×10 9 , 5.5×10 9 , 6×10 9 CFU / g, and then the fermentation broth was centrifuged at 12,000 rpm for 5 min with a desktop centrifuge, and the supernatant was taken.

[0069]2. Mix the obtained supernatant according to the volume ratio of 3:3:4 to obtain the probiotic supernatant. The supernatant was sterilized at 95°C for 5 minutes, then cooled to room temperature, and its acidity was measured. The measured pH was 3.63, and the titrated acidity was 183.80°T.

[0070] 3. According to the acidity of the centrifuged supernatant after sterilization, calculate the theoretically neutralized NaHCO per gram of the...

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Abstract

The invention discloses a skin micro-ecological improvement preparation and a preparation method thereof. The skin micro-ecological improvement preparation is formed by dissolving probiotic tablets in pre-essence; the skin micro-ecological improvement preparation contains 1*10<6> to 1*10<8> CFU/g active probiotics; the pH of the skin micro-ecological improvement preparation is 5.0 to 5.6. The invention also discloses a preparation method of the skin micro-ecological improvement preparation. According to the skin micro-ecological improvement preparation, probiotic fermentation culture supernate is taken as the pre-essence of probiotics effervescent tablets, and simultaneously, a NaHCO3 buffering agent is added into the tablets, so that a weak acidic environment can be maintained; the damage of residues of alkali cleanser products to skin is reduced. Meanwhile, the micro-ecological improvement preparation disclosed by the invention can ensure the activity and the stable number of the probiotics in production and product storage processes; the micro-ecological balance of the skin is regulated fundamentally; the skin health is maintained; meanwhile, the skin micro-ecological improvement preparation has multi-effect repair functions of moisturizing, softening skin, tightening and the like; a new idea is provided for solving skin problems.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a preparation for improving skin microecology and a preparation method thereof. Background technique [0002] The influence of microbes on the skin is rarely paid attention to by the beauty industry. However, with the advancement of life sciences, researchers in the fields of medicine and biology have discovered that microbes play a vital role in the human body and life. The quality of human skin is inseparable from microbes. Relationship. Skin microbes play a key role in the restoration and reconstruction of microecological balance, and at the same time play an extremely important role in the formation of biological barriers, the decomposition of sebaceous glands, and the formation of acid mantles. The skin microbiota maintains a coordinated, physiological and dynamic balance with the host and the environment, which is beneficial to the host. This balance is the skin...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/73A61K8/365A61K8/19A61Q19/00
Inventor 胡鹏
Owner 深圳安德瑞医药科技有限公司
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