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Micro-ecological additive for low-salt pickled vegetables and preparation method of micro-ecological additive

An additive and micro-ecological technology, which is applied to the field of low-salt pickle micro-ecological additives and preparation thereof, can solve the problems of long fermentation period of low-salt pickles, potential safety hazards of pathogenic bacteria, and high nitrite content, and achieves good thermal stability and Acid-base resistance, improved safety, small molecular weight effect

Active Publication Date: 2017-05-31
SICHUAN DONGPO CHINESE PAOCAI IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a low-salt pickle micro-ecological additive and its preparation method in view of the potential safety hazards of pathogenic bacteria in low-salt pickles and the problem that the nitrite content in low-salt pickles is higher than the national standard
The low-salt kimchi micro-ecological additive can solve the problems of low-salt kimchi that are blooming and sticky due to frequent vegetable changes, and make the fermentation cycle of low-salt kimchi longer under low temperature conditions

Method used

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  • Micro-ecological additive for low-salt pickled vegetables and preparation method of micro-ecological additive
  • Micro-ecological additive for low-salt pickled vegetables and preparation method of micro-ecological additive
  • Micro-ecological additive for low-salt pickled vegetables and preparation method of micro-ecological additive

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Effect test

Embodiment 1

[0027] Screening and Identification of Bacteriostatic Lactobacillus Pentosus

[0028] Take 25mL of pickle water from the upper, middle and lower layers of the pickle altar, put it in 225mL sterilized normal saline, mix it evenly, and then use sterilized normal saline to carry out gradient dilution to 10 -6 100 μL of each dilution sample was spread on the MRS plate, and incubated at 37°C for 48h. Pick a single colony that is visible to the naked eye on the culture medium, streak it, purify it repeatedly until there are no other bacteria, record the status of different colonies, and use Gram staining for microscopic examination to observe its morphological characteristics. Numbered and stored in glycerol in a ‐80°C refrigerator, isolated and purified Lactobacillus pentosus.

[0029] Lactobacillus pentosus was inoculated into 100 mL of sterilized liquid culture solution with an inoculation amount of 2%, and cultured statically at 37° C. for 48 hours to obtain a seed solution. T...

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Abstract

The invention belongs to the field of food, especially belongs to the field of pickled vegetable fermentation biotechnology and particularly relates to a micro-ecological additive for low-salt pickled vegetables and a preparation method of the micro-ecological additive. The low-salt pickled vegetable micro-ecological additive comprises lactobacillus pentosus which is classified and named as Lactobacillus pentosus PCYSWX-5 and preserved in the China General Microbiological Culture Collection Center on July 15th, 2016, and the preservation number of the lactobacillus pentosus is CGMCC No. 12793. The prepared micro-ecological additive has the advantages that the micro-ecological additive can inhibit or kill common pathogenic bacteria such as escherichia coli, staphylococcus aureus, enterobacter sakazakii, listeria monocytogenesis and salmonella, does not have an inhibition effect on main fermentation bacteria, and can increase the eating safety of the pickled vegetables; the additive can intensely inhibit or kill nitrate reducing bacteria in the low-salt pickled vegetables in the initial stage of fermentation to allow the nitrate content in the low-salt pickled vegetables to be lower than national standards.

Description

technical field [0001] The invention belongs to the field of food, in particular to the technical field of kimchi fermentation in food, and specifically relates to a low-salt kimchi microecological additive and a preparation method thereof. Background technique [0002] Kimchi is a kind of traditional fermented vegetable product, which spreads all over the country and has always been an indispensable side dish. However, due to the excessive intake of salt by the human body, the risk of suffering from high blood pressure, cardiovascular and cerebrovascular diseases and bone diseases will be increased, so the low-saltization process (salt content 0.5-3%) will be one of the important directions for the development of the traditional kimchi industry. However, pickles have low salinity and weak microbial inhibition ability. In the early stage of fermentation, bacteria on the surface of vegetables grow rapidly, and there is a potential safety hazard that the nitrite content exceed...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00A23L19/20C12R1/225
CPCC12N1/20C12P21/00C12N1/205C12R2001/225
Inventor 唐垚陈功张其圣李恒汪冬冬伍亚龙
Owner SICHUAN DONGPO CHINESE PAOCAI IND TECH RES INST
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