Micro-ecological additive for low-salt pickled vegetables and preparation method of micro-ecological additive
An additive and micro-ecological technology, which is applied to the field of low-salt pickle micro-ecological additives and preparation thereof, can solve the problems of long fermentation period of low-salt pickles, potential safety hazards of pathogenic bacteria, and high nitrite content, and achieves good thermal stability and Acid-base resistance, improved safety, small molecular weight effect
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[0027] Screening and Identification of Bacteriostatic Lactobacillus Pentosus
[0028] Take 25mL of pickle water from the upper, middle and lower layers of the pickle altar, put it in 225mL sterilized normal saline, mix it evenly, and then use sterilized normal saline to carry out gradient dilution to 10 -6 100 μL of each dilution sample was spread on the MRS plate, and incubated at 37°C for 48h. Pick a single colony that is visible to the naked eye on the culture medium, streak it, purify it repeatedly until there are no other bacteria, record the status of different colonies, and use Gram staining for microscopic examination to observe its morphological characteristics. Numbered and stored in glycerol in a ‐80°C refrigerator, isolated and purified Lactobacillus pentosus.
[0029] Lactobacillus pentosus was inoculated into 100 mL of sterilized liquid culture solution with an inoculation amount of 2%, and cultured statically at 37° C. for 48 hours to obtain a seed solution. T...
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