A kind of inoculum composition and its method for constructing xenograft tumor animal model derived from malignant pleural effusion
A malignant pleural effusion and animal model technology, applied in the field of xenograft tumor animal model construction, can solve the problems of killing tumor cells, and the inability to construct xenograft tumor animal models for malignant pleural effusion, so as to improve the success rate and make the establishment process simple and easy line, retain the effect of cell biological activity
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Embodiment 1
[0079] Example 1 Construction of xenograft tumor mouse model derived from malignant pleural effusion of lung cancer
[0080] 1. experimental animals
[0081] Order 4-5-week-old female severe combined immunodeficiency mice from the supplier and keep them in an SPF-level animal room. The animals are adaptively fed for at least three days before the start of the experiment.
[0082] 2. experiment procedure
[0083] 2.1 Collection of clinical malignant pleural effusion samples for lung cancer
[0084] Collect 200ml freshly collected malignant pleural effusion samples from non-small cell lung cancer in test tubes containing anticoagulants, transport them on ice, and transport them to the laboratory within 24 hours. A total of 15 cases of malignant pleural effusion were collected.
[0085] 2.2 Enrichment of tumor cells in malignant pleural effusion
[0086] Dilute the malignant pleural effusion sample with one volume of PBS buffer (HyClone, SH30256.01) in a biological safety...
Embodiment 2
[0097] Example 2 Drug efficacy experiment carried out on non-small cell lung cancer model CTC15012
[0098] In order to verify that the lung cancer mutation model established by the present invention is consistent with the clinical response, corresponding drug efficacy experiments consistent with the drugs taken by clinical patients are carried out on the established animal model.
[0099] Female NU / NU nude mice were subcutaneously inoculated with human ALK fusion gene model CTC15012 tumor mass. The non-small cell lung cancer model CTC15012 was derived from a 55-year-old female patient whose pathological diagnosis was bronchopulmonary adenocarcinoma. Cut the tumor into small pieces in HBSS and inoculate it under the skin of nude mice until the tumor grows to 500-700mm 3 When , the tumor was cut into small pieces of 10-15 mg in HBSS culture medium and used for subcutaneous inoculation in 20 experimental nude mice. The specific inoculation method is: disinfect the skin on both...
Embodiment 3
[0104] Example 3 Drug efficacy experiment carried out on non-small cell lung cancer model CTC15008
[0105] Female NU / NU nude mice were subcutaneously inoculated with human EGFR exon 21 L858R mutation model CTC15008 tumor mass. The non-small cell lung cancer model CTC15008 was derived from a 71-year-old female patient whose pathological diagnosis was bronchopulmonary adenocarcinoma. Cut the tumor into small pieces in HBSS and inoculate it under the skin of nude mice until the tumor grows to 500-700mm 3 When , the tumor was cut into small pieces of 10-15 mg in HBSS culture medium and used for subcutaneous inoculation in 20 experimental nude mice. The specific inoculation method is: disinfect the skin on both sides of the back of the mouse with povidone iodine, and use a No. 20 trocar to inject a total amount of 2 × 2 × 2mm 3 The tumor tissue is pierced on both sides of the back, the needle travels between the skin and the muscle, and finally the tissue is sent under the skin ...
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