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Internal standard detection method for furacilin biomarker NF (5-nitro-2-furaldehyde)

A nitrofurazone biological and detection method technology, applied in the detection field of nitrofurazone biomarker 5-nitro-2-furfural, can solve problems such as indistinguishability, and achieve the effect of breaking through limitations

Active Publication Date: 2017-05-31
MARINE FISHERIES RES INST OF ZHEJIANG
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Problems solved by technology

[0003] At present, the detection methods for illegal drugs of nitrofurazone mainly include high performance liquid chromatography and mass spectrometry, ultraviolet and fluorescence detectors, spectrophotometry, thin layer chromatography and immunoassay, etc., based on semicarbazide (SEM) as a biomarker The detection method cannot distinguish whether the "positive" SEM is from nitrofurazone or naturally occurring
This has also caused great controversy in the industry whether the SEM that has been used until now can continue to be used as a marker for nitrofurazone detection

Method used

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  • Internal standard detection method for furacilin biomarker NF (5-nitro-2-furaldehyde)
  • Internal standard detection method for furacilin biomarker NF (5-nitro-2-furaldehyde)
  • Internal standard detection method for furacilin biomarker NF (5-nitro-2-furaldehyde)

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Embodiment

[0028] An internal standard detection method of nitrofurazone biomarker 5-nitro-2-furfural, comprising the steps of:

[0029] (1) Reagent preparation

[0030] Dissolve 100 mg of DNPH (2,4-dinitrophenylhydrazine) in 1 ml of concentrated hydrochloric acid (12M), then dilute to a 100 ml volumetric flask with methanol to obtain a derivatization concentration of 1.0 mg / mL Reagent solution;

[0031] (2) Derivatization and purification

[0032] Weigh 1.0±0.1g of sample, put it into a 50ml sample tube, add 10ml of hydrochloric acid (0.2mol / L), 100μL of derivatization reagent solution, 100μL of PDAB solution (1.0μg / mL), vortex fully, avoid Light was placed in a water bath at 60°C for 20 minutes, followed by centrifugation at 6000 rpm for five minutes, and the supernatant was purified by Oasis PRiME HLB solid-phase extraction cartridge (Waters), and the obtained liquid was rotary evaporated to dryness. The residue was redissolved with 1.0 ml of mobile phase (acetonitrile / water=7 / 3, V...

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Abstract

The invention discloses an internal standard detection method for a furacilin biomarker NF (5-nitro-2-furaldehyde). NF is taken as a detection object, and 2,4-dinitrophenylhydrazine is adopted as a derivatization reagent to convert a target object into a stable compound which responds sensitively under a detector. Accordingly, the limitation caused by the fact that endogenous non-furacilin biomarkers and the like cannot be prevented from being generated in existing research is broken through.

Description

technical field [0001] The invention relates to a detection method of nitrofurazone biomarker 5-nitro-2-furfural, in particular to an internal standard detection method of nitrofurazone biomarker 5-nitro-2-furfural. Background technique [0002] The chemical properties of nitrofuran drugs are unstable, especially sensitive to light, etc., and the metabolism rate in the organism is very fast, the half-life of the concentration ranges from a few hours to more than ten hours, and it is basically undetectable in the animal body after 24 hours of feeding the drug Presence of unchanged nitrofuran. Therefore, it is difficult and inaccurate to detect the original drug of nitrofuran from the feed or in the treated animals, and the residual amount of the original drug is not enough to reflect the real drug use. However, the metabolites of nitrofuran can exist in the organism for a long time after being combined with tissue proteins. Studies have shown that stable levels of metabolite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张帅张小军梅光明严忠雍龙举郭远明
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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