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TLC (Thin-Layer Chromatography) detection method for Chinese angelica in herba leonuri biochemical mixture

A Yimu biochemical and thin-layer chromatography technology, which is applied in the detection field of Angelica TLC in Yimusheng chemical mixture, can solve the problems of unclear spots and slow expansion speed, so as to improve the detection accuracy, increase the expansion speed, The effect of increasing the unfolding speed

Inactive Publication Date: 2017-05-31
SICHUAN DECHENG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: there are problems in the prior art that the development speed becomes slow and the spots are not clear, and the purpose is to provide a TLC detection method for Angelica sinensis in a Yimusheng chemical mixture that solves the above problems

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  • TLC (Thin-Layer Chromatography) detection method for Chinese angelica in herba leonuri biochemical mixture
  • TLC (Thin-Layer Chromatography) detection method for Chinese angelica in herba leonuri biochemical mixture

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Embodiment 1

[0022] A kind of TLC detection method of Angelica sinensis in Yimusheng chemical mixture, the specific detection process is as follows:

[0023] Take an appropriate amount of Yimusheng Chemical Mixture as the test solution; take 1 / 20 of the amount of prescription medicinal materials (except Angelica) to make a negative control solution according to the preparation process of Yimusheng Chemical Mixture. Another appropriate amount of Chinese angelica reference medicinal material was taken, and the reference substance solution was prepared according to the preparation process of Yimusheng chemical mixture. Take 10 μL of each of the above three solutions, and spot them on the same silica gel G thin-layer plate, using n-hexane and ethyl acetate as developing solvents, wherein n-hexane: ethyl acetate = 4:1, develop, take out, and dry in the air. Check under UV light at 365nm. In the chromatogram of the test product, at the position corresponding to the chromatogram of the control m...

Embodiment 2

[0027] This embodiment is a comparative example of embodiment 1. In this embodiment, the specific ratio of developing agent is different, and the specific detection process is as follows:

[0028] Take an appropriate amount of Yimusheng Chemical Mixture as the test solution; take 1 / 20 of the amount of prescription medicinal materials (except Angelica) to make a negative control solution according to the preparation process of Yimusheng Chemical Mixture. Another appropriate amount of Chinese angelica reference medicinal material was taken, and the reference substance solution was prepared according to the preparation process of Yimusheng chemical mixture. Take 10 μL of each of the above three solutions, and spot them on the same silica gel G thin-layer plate, using n-hexane and ethyl acetate as developing solvents, wherein n-hexane: ethyl acetate = 5:1, develop, take out, and dry in the air. Check under UV light at 365nm. In the chromatogram of the test product, at the positio...

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Abstract

The invention discloses a TLC (Thin-Layer Chromatography) detection method for Chinese angelica in a herba leonuri biochemical mixture and solves the problems existing in the prior art that the development rate is slowed down and spots are not clear. The TLC detection method comprises the following steps: (1) preparation of a testing product solvent: taking and preparing the herba leonuri biochemical mixture into the testing product solvent; (2) sucking and dripping the testing product solvent on a silica gel thin-layer plate, taking normal hexane and ethyl acetate which are 4 to 1 in proportion as a developing agent, developing, taking out and airing; (3) carrying out visual inspection under an ultraviolet lamp. According to the TLC detection method disclosed by the invention, the developing efficiency and the developing effect can be effectively increased, and the effect is very remarkable.

Description

technical field [0001] The invention relates to a thin-layer chromatography detection method, in particular to a thin-layer chromatography detection method of angelica in Yimusheng chemical mixture. Background technique [0002] Yimusheng Chemical Mixture is made from more than six kinds of herbs including motherwort, angelica, chuanxiong, peach kernel, paojiang, and licorice (burnt). It is a pale orange-yellow to brown-yellow liquid. The gas is fragrant and the taste is slightly sweet. It has the functions of promoting blood circulation and removing stasis, warming meridians and relieving pain. Indications for postpartum lochia, blood stasis and abdominal pain. The Yimusheng chemical mixture is mainly used for livestock such as horses, cattle, sheep and pigs, wherein the dosage for horses and cattle is 200-300mL, and the dosage for sheep and pigs is 30-50mL. [0003] At present, the preparation is recorded in the "National Standards Collection of Veterinary Drugs - Nati...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 潘春晖张会峰杨琼雍玲刘涛张煊任园琴
Owner SICHUAN DECHENG ANIMAL HEALTH PROD
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