A colloidal gold detection method and system based on image processing

Abstract

The invention discloses a method and a system for detecting colloidal gold based on image processing. The method comprises the following steps: carrying out gray-level treatment on an area to be identified of detection images of a colloidal gold detection card and calculating a gray-level integral curve, searching all wave trough points of the gray-level integral curve, and calculating half peak width, peak center point and effective integral area of each wave trough point; judging validity of the colloidal gold detection card according to the half peak width of a maximum wave trough point, judging and searching a C-line position and a T-line position according to the position of the peak center point of the maximum wave trough point so as to achieve qualitative analysis of the colloidal gold detection card, wherein the obtained image limiting conditions are few, the C line and the T line are accurately positioned, identified and analyzed, the compatibility is high, and the application range is wide; and further calculating the ratio of the gray-level integral value of the T-line position to the gray-level integral value of the C-line position, comparing the ratio with a standard ratio to obtain the concentration value of samples to be detected and achieve quantitative analysis of the colloidal gold detection card. The detection precision is achieved.

Description

technical field [0001] The invention relates to the technical field of colloidal gold immunochromatography, in particular to a colloidal gold detection method based on image processing and a system for applying the method. Background technique [0002] In the field of rapid detection of food safety and circulation, colloidal gold immunochromatography technology has been widely used in the detection of drug residues in tissues, urine, and blood due to its advantages of simple operation and convenient interpretation, such as clenbuterol, Ractopamine, tetracycline, etc. The principle is to immobilize the specific antibody on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose is immersed in the sample, the sample will move forward along the membrane, and when it moves to the area where the antibody is immobilized At this time, the corresponding antigen in the sample is specifically combined with the antibody, and the antigen antibody...

AI Technical Summary

Problems solved by technology

The test result is to observe the color depth of the C line and T line of the reaction plate. The smaller the color ratio of T line / C line, the higher the concentration of the substance to be tested. At present, most of them are only judged by naked eyes, which is highly subjective. , resulting in a relatively limited scope of application

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