Peptide and application thereof

An amino acid and sequence technology, applied in the protein field, can solve the problems of restricting the promotion of EGF cosmetics, restricting the clinical research and clinical application of EGF, high price, etc., and achieves the effect of small side effects, accurate curative effect, and promotion of proliferation.

Inactive Publication Date: 2017-06-06
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to technical and other reasons, the production and supply of EGF raw materials is difficult to meet the growing demand, and the price of EGF has remained

Method used

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  • Peptide and application thereof
  • Peptide and application thereof
  • Peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Computer Molecular Simulation Screening Experiment for Functional Short Peptides

[0049] 1.1 Experimental materials

[0050] Supercomputer equipped with 192AMD Opteron processor group

[0051] 1.2 Experimental method:

[0052] The principle of high-throughput computer molecular simulation screening technology is as follows: (1) Preliminary screening of pharmacophore and target. That is to use the existing Chinese herbal medicine molecular monomer database to conduct a comprehensive analysis of receptors such as proteins. Simulate the interaction between Chinese herbal medicine monomer molecules and target receptor protein molecules, and obtain the scores and action sites of these substances. (2) Molecular docking. Apply the semi-flexible docking method, by continuously optimizing the position (orientation) of the small molecule compound and the dihedral angle (conformation) of the flexible bond inside the molecule, to find the best conformation for the i...

Embodiment 2

[0084] Example 2: Effect of Arg-Arg-Tyr on the proliferation of human skin fibroblasts

[0085] 1 material

[0086] 1.1 Cell lines

[0087] Human skin fibroblast cell line (HSF) was purchased from the IBS Cell Resource Center of Fudan University.

[0088] 1.2 Biochemical reagents

[0089] Penicillin-Streptomycin Solution (100×), Trypsin Cell Digestion Solution (containing phenol red), Phosphate-Buffered Saline (containing 135mM NaCl, 4.7mM KCl, 10mM NaCl 2 HPO 4 , 2mM NaH 2 PO 4 , pH value is 7.4), all are products of Biyuntian Biotechnology Co., Ltd.; DMEM high-glucose medium, cell cryopreservation solution, products of Gibco company in the United States; premium fetal bovine serum in Australia, Gemini company in the United States; TritonX-100, bovine serum Albumin (BSA) was from Guangzhou Jietewei Biotechnology Co., Ltd.; sodium chloride, absolute ethanol, paraformaldehyde, and dimethyl sulfoxide (DMSO) were all products from Guangzhou Chemical Reagent Co., Ltd., analy...

Embodiment 3

[0209] Embodiment 3 animal test

[0210] 1 Materials and methods

[0211] 1.1 Experimental animals: 18 SD mice, female, weighing 18.0±0.6g, were purchased from the Animal Experiment Center of Sun Yat-sen University.

[0212] 1.2 Experimental reagents: Arg-Arg-Tyr (RRY) with a purity of 99.33%, was synthesized by Shanghai Qiangyao Biotechnology Co., Ltd.; it was prepared as a 1 μM solution with distilled water.

[0213] 1.3 Instruments:

[0214] Leica paraffin microtome, product of Leica, Germany; Shandon Paraffin Section Flotation Bath, product of Thermo Fisher Scientific, USA; DM 2500B upright microscope imaging system, product of Leica, Germany.

[0215] 1.4 Experimental grouping and processing:

[0216] Using random number method, 12 SD mice were randomly divided into 2 groups, 6 in each group: blank control group: no treatment; 1 μM Arg-Arg-Tyr solution short peptide group. The above-mentioned groups were shaved on the back one day before the experiment (throughout the...

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Abstract

The invention relates to the protein field, in particular to a peptide and application thereof. According to the invention, tripeptide Arg-Arg-Tyr acts on human skin fibroblasts to perform CCK-8 proliferation assay, cell proliferation detection assay by Edu process, and cell cycle detection assay by flow cytometry on human skin fibroblasts. The assays prove that the tripeptide Arg-Arg-Tyr can effectively promote the proliferation of human skin fibroblasts and increase the proportion of S+G2M phase, has definite therapeutic effect on prevention or treatment of skin aging, and has small side effect, thus having important practical significance.

Description

technical field [0001] The present invention relates to the protein field, in particular to a peptide and its application. Background technique [0002] Aging is a process in which the functions of various organs in the body generally decrease gradually. Aging has two different situations, one is physiological aging that occurs under normal circumstances; the other is pathological aging caused by diseases. is a law of nature. However, when people adopt good living habits and health care measures, they can effectively delay aging and improve the quality of life. The theory of traditional Chinese medicine believes that the growth, development and aging of the human body are closely related to the viscera functions and the ups and downs of meridian qi and blood. When the body’s qi and blood are insufficient, the meridian qi is not running smoothly, the function of the viscera is weakened, and the balance of yin and yang is out of balance, all of which will lead to and accele...

Claims

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Application Information

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IPC IPC(8): C07K5/09A61K8/64A61Q19/08
CPCA61K8/64A61Q19/08C07K5/0817
Inventor 华洋林王子偲唐健顾怀宇
Owner INFINITUS (CHINA) CO LTD
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