Method for performing ultrasonic-fluorescent detection to content of petroleum hydrocarbon in polychaete body

Abstract

The invention discloses a method for performing ultrasonic-fluorescent detection to the content of petroleum hydrocarbon in polychaete body. The method comprises the following steps: preparing an oil standard solution and drawing a standard curve; drying polychaete into powder for 16 hours under the condition of 105 DEG C, mixing a powdery polychaete sample with chromatographically pure dichloromethane and performing ultrasonic extraction, collecting supernatant, equilibrating a 60-120-mesh silica gel chromatographic column for 8-12 hours with chromatographically pure dichloromethane, eluting with 20 ml of dichloromethane, then performing evaporation through a rotating evaporator, diluting with n-hexane to 10ml, filtering through a nylon film with aperture of 0.22 mu m, and performing fluorescence spectrophotometric determination under the conditions that excitation wavelength is 310 nm, the emission wavelength is 360 nm, the slit is set to be 1nm, the scanning speed is 1200 nm / min, PMT is 700 V; and finding out corresponding values from the standard curve, and calculating the content of petroleum hydrocarbon in polychaete sample.

Description

technical field [0001] The invention belongs to marine environmental pollution monitoring technology, and mainly relates to a method for measuring the content of marine invertebrate polychaete petroleum hydrocarbons. Background technique [0002] Petroleum hydrocarbons are one of the main substances that cause marine pollution. Petroleum hydrocarbons in the ocean are enriched in marine organisms and can be transmitted through the food chain and cause harm to the human body. Polychaetes belong to the phylum Annelida, and are dominant benthic animals widely distributed in the coastal and estuary tidal flats. Due to their large size, long life cycle, and slow movement, they are very easy to contact and enrich in sediments. Petroleum hydrocarbons in the body. Studies have shown that marine polychaetes can ingest a large amount of sediment and excrete it into non-toxic metabolites through a series of transformation and decomposition, showing strong tolerance and bioavailability ...

AI Technical Summary

Problems solved by technology

[0003] At present, the determination of petroleum hydrocarbon content in marine organisms uses saponification method to process samples, but the difference in saponification effect will lead to huge differences in the determination of petroleum hydrocarbon content in samples.

If the saponification effect is not good, the extract will appear more serious brownish yellow, which will make the emulsification phenomenon serious, affect the extraction effect, and thus affect the measurement results

In addition, the saponification process is more complicated and time-consuming. During the saponification extraction process, the order of adding reagents, the quality and quantity of water added, the amount of sampling, saponification time, temperature, and the number of extractions will all affect the accuracy of the final measurement results.

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