Method for preparing pectin oligosaccharide having molecular weight of 2000-3000 Da through acid hydrolysis
A polysaccharide acid and molecular weight technology, applied in the field of food chemistry, can solve the problems of uncontrollable hydrolysis process, high cost, and difficulty in obtaining active oligosaccharides, and achieve the effect of short production cycle, low cost, and good antibacterial activity
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Embodiment 1
[0020] The preparation process steps of pectin oligosaccharides are as follows:
[0021] 1) Add 1 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 2 mol / L, so that the quality of citrus pectin is 1% of the volume of the trifluoroacetic acid aqueous solution, mix and react in a shaking table under 85 ° C water bath conditions 2.5 hours;
[0022] 2) cooling the reaction solution, centrifuging, and taking the supernatant;
[0023] 3) Add 8 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate, and dry it at 40°C to obtain the final product.
Embodiment 2
[0025] The preparation process steps of pectin oligosaccharides are as follows:
[0026] 1) Add 2 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 1 mol / L, so that the quality of citrus pectin is 2% of the volume of the trifluoroacetic acid aqueous solution, mix and react in a shaking table under 80° C. water bath condition 3h;
[0027] 2) cooling the reaction solution, centrifuging, and taking the supernatant;
[0028] 3) Add 5 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate, and dry it at 40°C to obtain the final product.
Embodiment 3
[0030] The preparation process steps of pectin oligosaccharides are as follows:
[0031] 1) Add 0.5 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 3 mol / L, so that the quality of citrus pectin is 0.5% of the volume of the trifluoroacetic acid aqueous solution, and shake the bed under 90° C. water bath condition after mixing Reaction 2h;
[0032] 2) cooling the reaction solution, centrifuging, and taking the supernatant;
[0033] 3) Add 10 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate and dry it at 50°C to obtain the final product.
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