Method for preparing pectin oligosaccharide having molecular weight of 2000-3000 Da through acid hydrolysis

A polysaccharide acid and molecular weight technology, applied in the field of food chemistry, can solve the problems of uncontrollable hydrolysis process, high cost, and difficulty in obtaining active oligosaccharides, and achieve the effect of short production cycle, low cost, and good antibacterial activity

Inactive Publication Date: 2017-06-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a pectin oligosaccharide with a molecular weight of 2000 to 3000 Da in view of the shortcomings of the existing pectin oligosaccharide preparation process, such as high cost, uncontrollable hydrolysis process, and difficulty in obtaining the active oligosaccharide of the required degree of polymerization and structure type. Gum oligosaccharide chemical acid hydrolysis preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation process steps of pectin oligosaccharides are as follows:

[0021] 1) Add 1 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 2 mol / L, so that the quality of citrus pectin is 1% of the volume of the trifluoroacetic acid aqueous solution, mix and react in a shaking table under 85 ° C water bath conditions 2.5 hours;

[0022] 2) cooling the reaction solution, centrifuging, and taking the supernatant;

[0023] 3) Add 8 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate, and dry it at 40°C to obtain the final product.

Embodiment 2

[0025] The preparation process steps of pectin oligosaccharides are as follows:

[0026] 1) Add 2 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 1 mol / L, so that the quality of citrus pectin is 2% of the volume of the trifluoroacetic acid aqueous solution, mix and react in a shaking table under 80° C. water bath condition 3h;

[0027] 2) cooling the reaction solution, centrifuging, and taking the supernatant;

[0028] 3) Add 5 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate, and dry it at 40°C to obtain the final product.

Embodiment 3

[0030] The preparation process steps of pectin oligosaccharides are as follows:

[0031] 1) Add 0.5 g of citrus pectin to 100 ml of trifluoroacetic acid aqueous solution with a concentration of 3 mol / L, so that the quality of citrus pectin is 0.5% of the volume of the trifluoroacetic acid aqueous solution, and shake the bed under 90° C. water bath condition after mixing Reaction 2h;

[0032] 2) cooling the reaction solution, centrifuging, and taking the supernatant;

[0033] 3) Add 10 times the volume of ethanol to the supernatant, let it stand overnight to precipitate, centrifuge to collect the precipitate and dry it at 50°C to obtain the final product.

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PUM

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Abstract

The invention discloses a method for preparing a pectin oligosaccharide having a molecular weight of 2000-3000 Da through acid hydrolysis. The method comprises the following steps: reacting citrus pectin and trifluoroacetic acid, adding ethanol into the supernate after the reaction to precipitate, and drying the precipitate. According to the invention, the controllable degradation of pectin is realized, and the pectin oligosaccharide having a weight average molecular weight of 2000-3000 Da is prepared, thereby improving the product quality; and tests prove that the pectin oligosaccharide has a better antibacterial activity than a product prepared through a common chemical method.

Description

technical field [0001] The invention belongs to the field of food chemistry and relates to a preparation method of pectin oligosaccharides, in particular to a method for preparing pectin oligosaccharides with a molecular weight of 2000-3000 Da by acid hydrolysis. [0002] technical background [0003] Pectin oligosaccharides are low-molecular-weight oligosaccharide products (molecular weight between 400-4000Da) after depolymerization of pectin, consisting of 2-20 α-1,4-D-galacturonic acid residues , is the first plant oligosaccharide discovered. Pectin oligosaccharides can be obtained by degrading pectin by biological enzymatic or chemical methods, and have many biologically active functions. As a new type of functional oligosaccharides, they can be used as plant regulators, prebiotics, antibacterial agents, etc. Broad application prospects. [0004] At present, pectin oligosaccharides are mainly extracted from natural plants, chemically, and enzymatically synthesized. Base...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
CPCC08B37/0045
Inventor 潘思轶胡海娟张珊珊范传会王鲁峰王可兴
Owner HUAZHONG AGRI UNIV
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