Method for separating and preparing primary liver cells

Abstract

The invention discloses a method for separating and preparing primary liver cells. In the method for separating and preparing the primary liver cells, composite enzymes are used for liver perfusion isolation to obtain the primary liver cells. The method belongs to the technical field of medical biology, the method for separating and preparing the primary liver cells can be used for effective separation of the primary liver cells, by interaction of the composite enzymes, different surface growth matrix of each cell can be fully hydrolyzed so as to obtain a single cell suspension. A suitable environment is provided for action of the composite enzymes by matching of a buffer system, so that enzymolysis is more complete, and the yield of the isolated primary liver cells is improved. A necessary environment is provided for cell life by catalytic properties of the composite enzymes and the buffer system, and the isolated primary liver cells have a higher survival rate.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a method for separating and preparing liver primary cells. Background technique [0002] As an important organ of the body, the liver plays a very important role in metabolism, digestion, detoxification, blood coagulation, immunity, and regulation. The liver is composed of hepatocytes, which perform the main functions of the liver, such as removing toxins, participating in biosynthesis and biotransformation, metabolizing various nutrients, storing glucose, and secreting active substances that promote the growth of liver cells. Isolation of hepatocytes from the body is helpful for in vitro research in pharmacology, toxicology, immunology, cell biology, etc., and with the application of bioartificial liver in the field of liver failure, a large number of highly active primary hepatocytes are increasingly needed as biological material. Therefore, how to isolate and prepare pri...

AI Technical Summary

Problems solved by technology

The complexity of the liver is that non-parenchymal cells include: stellate cells, sinus endothelium, bile duct epithelium and immune cells (lymphocytes and granulocytes), while the entire liver group is composed of 60% parenchymal cells and 40% non-parenchymal cells A large network structure, a single enzymatic hydrolysis can not achieve good results, therefore, it is necessary to improve the method for the separation and preparation of primary liver cells in the prior art