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A kind of go/nha/collagen composite bone repair material and preparation method thereof

A technology for bone repair and collagen, which is applied in the field of biomedical composite materials, can solve problems such as cell membrane damage, and achieve the effects of simple operation, dense and orderly arrangement of collagen fibers, and excellent cross-linking effect

Active Publication Date: 2020-06-12
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, graphene oxide has certain biological toxicity, and

Method used

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  • A kind of go/nha/collagen composite bone repair material and preparation method thereof
  • A kind of go/nha/collagen composite bone repair material and preparation method thereof
  • A kind of go/nha/collagen composite bone repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] (1) Weigh 0.4 g of freeze-dried bovine tendon collagen and dissolve it in 99.6 mL of malonic acid with a volume concentration of 0.3% to obtain a collagen solution with a mass concentration of 0.4%, and continue stirring for 4 hours;

[0038] (2) Gradually add 0.08 g of nano-hydroxyapatite powder to the obtained collagen solution, magnetically stir for 8 hours, freeze-dry for 48 hours, and cross-link in 1000 mL of cross-linking solution;

[0039] (3) The composite membrane obtained after cross-linking is repeatedly washed with deionized water, and then freeze-dried again to obtain a nano-hydroxyapatite / collagen composite membrane with a GO content of 0 wt%.

Example Embodiment

[0040] Example 2

[0041] (1) Add 0.005 g of graphene oxide powder to water and ultrasonically disperse it for 2 hours to obtain a 2 mg / mL graphene oxide dispersion;

[0042] (2) Weigh 0.4 g of freeze-dried bovine tendon collagen and dissolve it in 99.6 mL of malonic acid with a volume concentration of 0.3% to obtain a collagen solution with a mass concentration of 0.4%;

[0043] (3) Mix the obtained graphene oxide dispersion with the collagen solution and stir for 4 hours to obtain a uniform graphene oxide-collagen mixture;

[0044] (4) Gradually add 0.095 g of nano-hydroxyapatite powder to the obtained graphene oxide-collagen mixture, magnetically stir for 8 h, freeze-dry for 48 h, and cross-link in 1000 mL of cross-linking solution;

[0045] (5) The composite film obtained after cross-linking is repeatedly washed with deionized water, and then freeze-dried again to obtain a graphene oxide / nano-hydroxyapatite / collagen composite film with a GO content of 1 wt%.

Example Embodiment

[0046] Example 3

[0047] (1) Add 0.01 g of graphene oxide powder to water and ultrasonically disperse it for 2 hours to obtain a 2 mg / mL graphene oxide dispersion;

[0048] (2) Weigh 0.4 g of freeze-dried bovine tendon collagen and dissolve it in 99.6 mL of malonic acid with a volume concentration of 0.3% to obtain a collagen solution with a mass concentration of 0.4%;

[0049] (3) Mix the obtained graphene oxide dispersion with the collagen solution and stir for 4 hours to obtain a uniform graphene oxide-collagen mixture;

[0050] (4) Gradually add 0.09 g of nano-hydroxyapatite powder to the obtained graphene oxide-collagen mixture, magnetically stir for 8 hours, freeze-dry for 48 hours, and cross-link in 1000 mL of cross-linking solution;

[0051] (5) The composite film obtained after cross-linking is repeatedly washed with deionized water, and then freeze-dried again to obtain a graphene oxide / nano-hydroxyapatite / collagen composite film with a GO content of 2 wt%.

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Abstract

The invention discloses a graphene oxide/nano-hydroxyapatite/collagen composite bone repair material and a preparing method thereof. Graphene oxide, nano-hydroxyapatite and collagen are used as raw materials, and mixing, stirring, freeze-drying, crosslinking and other steps are conducted to obtain the composite material. The mass ratio of graphene oxide to nano-hydroxyapatite to collagen is (2.5-5):(20-25):100. The composite material is of a loose and porous three-dimensional structure, the porosity reaches 90% or more, pores are interconnected inside, and in-vitro bioactivity and biocompatibility are high. Detection proves that the hydrophily, mechanical property and heat stability of the composite material are improved remarkably along with the increase of the GO content within a certain range. The composite material is a good medical material suitable for bone tissue defect repair.

Description

technical field [0001] The invention belongs to the field of biomedical composite materials, in particular to a graphene oxide / nano hydroxyapatite / collagen composite bone repair material and a preparation method thereof. Background technique [0002] A small area of ​​human bone tissue defect can be regenerated through self-healing ability, but this self-healing ability is limited by age, disease and other factors, so artificially synthesized bone repair and bone substitute materials have become the focus of attention since the 1960s . At present, constructing bone repair materials in vitro through tissue engineering to fill the defect site to treat bone tissue defects is becoming an ideal treatment method for repairing bone tissue defects. [0003] Collagen is the most abundant protein in mammals. It is the main component of extracellular matrix (ECM), the main component of skin, blood vessels, bones, tendons, teeth and cartilage. It is a structural protein and has the fun...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/44A61L27/50
CPCA61L27/443A61L27/46A61L27/50A61L2430/02C08L89/00
Inventor 王建华张其清孙晓敏杨秋张志华
Owner FUZHOU UNIVERSITY
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